Genetic Screening and Expression Analysis of Psychrophilic <i>Bacillus</i> spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat
Abiotic stress in plants pose a major threat to cereal crop production worldwide and cold stress is also notorious for causing a decrease in plant growth and yield in wheat. The present study was designed to alleviate cold stress on plants by inoculating psychrophilic PGPR bacteria belonging to Baci...
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MDPI AG
2019-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/7/9/337 |
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| author | Muhammad Zubair Alvina Hanif Ayaz Farzand Taha Majid Mahmood Sheikh Abdur Rashid Khan Muhammad Suleman Muhammad Ayaz Xuewen Gao |
| author_facet | Muhammad Zubair Alvina Hanif Ayaz Farzand Taha Majid Mahmood Sheikh Abdur Rashid Khan Muhammad Suleman Muhammad Ayaz Xuewen Gao |
| author_sort | Muhammad Zubair |
| collection | DOAJ |
| description | Abiotic stress in plants pose a major threat to cereal crop production worldwide and cold stress is also notorious for causing a decrease in plant growth and yield in wheat. The present study was designed to alleviate cold stress on plants by inoculating psychrophilic PGPR bacteria belonging to Bacillus genera isolated from extreme rhizospheric environments of Qinghai-Tibetan plateau. The genetic screening of psychrophilic <i>Bacillus</i> spp. CJCL2, RJGP41 and temperate <i>B. velezensis</i> FZB42 revealed presence of genetic features corresponding to cold stress response, membrane transport, signal transduction and osmotic regulation. Subsequently, the time frame study for the expression of genes involved in these pathways was also significantly higher in psychrophilic strains as analyzed through qPCR analysis at 4 ℃. The inoculated cold tolerant Bacillus strains also aided in inducing stress response in wheat by regulating abscisic acid, lipid peroxidation and proline accumulation pathways in a beneficial manner. Moreover, during comparative analysis of growth promotion in wheat all three Bacillus strains showed significant results at 25 ℃. Whereas, psychrophilic Bacillus strains CJCL2 and RJGP41 were able to positively regulate the expression of phytohormones leading to significant improvement in plant growth under cold stress. |
| first_indexed | 2024-12-11T16:25:53Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-12-11T16:25:53Z |
| publishDate | 2019-09-01 |
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| series | Microorganisms |
| spelling | doaj.art-b5a0d91e591c4c5b9e4cac1d9c6b06e92022-12-22T00:58:44ZengMDPI AGMicroorganisms2076-26072019-09-017933710.3390/microorganisms7090337microorganisms7090337Genetic Screening and Expression Analysis of Psychrophilic <i>Bacillus</i> spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in WheatMuhammad Zubair0Alvina Hanif1Ayaz Farzand2Taha Majid Mahmood Sheikh3Abdur Rashid Khan4Muhammad Suleman5Muhammad Ayaz6Xuewen Gao7Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaInstitute of Microbiology, School of life sciences, Lanzhou University, Lanzhou 730000, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaDepartment of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, ChinaAbiotic stress in plants pose a major threat to cereal crop production worldwide and cold stress is also notorious for causing a decrease in plant growth and yield in wheat. The present study was designed to alleviate cold stress on plants by inoculating psychrophilic PGPR bacteria belonging to Bacillus genera isolated from extreme rhizospheric environments of Qinghai-Tibetan plateau. The genetic screening of psychrophilic <i>Bacillus</i> spp. CJCL2, RJGP41 and temperate <i>B. velezensis</i> FZB42 revealed presence of genetic features corresponding to cold stress response, membrane transport, signal transduction and osmotic regulation. Subsequently, the time frame study for the expression of genes involved in these pathways was also significantly higher in psychrophilic strains as analyzed through qPCR analysis at 4 ℃. The inoculated cold tolerant Bacillus strains also aided in inducing stress response in wheat by regulating abscisic acid, lipid peroxidation and proline accumulation pathways in a beneficial manner. Moreover, during comparative analysis of growth promotion in wheat all three Bacillus strains showed significant results at 25 ℃. Whereas, psychrophilic Bacillus strains CJCL2 and RJGP41 were able to positively regulate the expression of phytohormones leading to significant improvement in plant growth under cold stress.https://www.mdpi.com/2076-2607/7/9/337psychrophilicPGPRgenetic screeningsignal transductionosmotic regulationplant-stress response |
| spellingShingle | Muhammad Zubair Alvina Hanif Ayaz Farzand Taha Majid Mahmood Sheikh Abdur Rashid Khan Muhammad Suleman Muhammad Ayaz Xuewen Gao Genetic Screening and Expression Analysis of Psychrophilic <i>Bacillus</i> spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat Microorganisms psychrophilic PGPR genetic screening signal transduction osmotic regulation plant-stress response |
| title | Genetic Screening and Expression Analysis of Psychrophilic <i>Bacillus</i> spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat |
| title_full | Genetic Screening and Expression Analysis of Psychrophilic <i>Bacillus</i> spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat |
| title_fullStr | Genetic Screening and Expression Analysis of Psychrophilic <i>Bacillus</i> spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat |
| title_full_unstemmed | Genetic Screening and Expression Analysis of Psychrophilic <i>Bacillus</i> spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat |
| title_short | Genetic Screening and Expression Analysis of Psychrophilic <i>Bacillus</i> spp. Reveal Their Potential to Alleviate Cold Stress and Modulate Phytohormones in Wheat |
| title_sort | genetic screening and expression analysis of psychrophilic i bacillus i spp reveal their potential to alleviate cold stress and modulate phytohormones in wheat |
| topic | psychrophilic PGPR genetic screening signal transduction osmotic regulation plant-stress response |
| url | https://www.mdpi.com/2076-2607/7/9/337 |
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