A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments
Abstract This study aimed to develop and validate a novel rabbit fixator made from a thermoplastic mask for awake imaging experiments. When heated in a hot-water bath at 65–70 °C for 2–5 min, the thermoplastic mask became soft and could be molded to fit over the entire body of an anesthetized rabbit...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-81358-6 |
| _version_ | 1819120164609720320 |
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| author | Rencai Lu Li Hou Siyu Wang Bo She Hong He Wentao Gao Sidang Wang Dongdong Xv Yunhai Ji Shasha Yang Zhaohui Yang Shaobo Wang |
| author_facet | Rencai Lu Li Hou Siyu Wang Bo She Hong He Wentao Gao Sidang Wang Dongdong Xv Yunhai Ji Shasha Yang Zhaohui Yang Shaobo Wang |
| author_sort | Rencai Lu |
| collection | DOAJ |
| description | Abstract This study aimed to develop and validate a novel rabbit fixator made from a thermoplastic mask for awake imaging experiments. When heated in a hot-water bath at 65–70 °C for 2–5 min, the thermoplastic mask became soft and could be molded to fit over the entire body of an anesthetized rabbit (4 ml of 3% pentobarbital sodium solution by intramuscular injection). Twenty rabbits were randomly divided into fixator (n = 10) and anesthesia (n = 10) groups. The animals’ vital signs, stress hormones (cortisol and adrenaline), and subjective image quality scores for the computed tomography (CT), positron emission tomography (PET), and magnetic resonance imaging (MRI) scanning were measured and compared. Phantom CT, MRI and PET studies were performed to assess the performance with and without the thermoplastic mask by using image agents at different concentrations or with different radioactivity. The respiration rate (RR), systolic blood pressure (SBP), diastolic blood pressure (DBP), peripheral capillary oxygen saturation (SpO2) and body temperature (T) decreased after anesthesia (all P < 0.05) but did not significantly decrease after fixation (all P > 0.05). The heart rate (HR), cortisol and adrenaline did not significantly decrease after either anesthesia or fixation (all P > 0.05). The subjective image quality scores for the CT and MRI images of the head, thorax, liver, kidney, intestines and pelvis and the subjective image quality scores for the PET images did not significantly differ between the two groups (all P > 0.05). For all examined organs except the muscle, 18F-FDG metabolism was lower after fixation than after anesthesia, and was almost identical of liver between two groups. The phantom study showed that the CT values, standard uptake values and MR T2 signal values did not differ significantly with or without the mask (all P > 0.05). A novel rabbit fixator created using a thermoplastic mask could be used to obtain high-quality images for different imaging modalities in an awake and near-physiological state. |
| first_indexed | 2024-12-22T06:16:19Z |
| format | Article |
| id | doaj.art-b5a1c68d76be4a47b4c4388511056e40 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-22T06:16:19Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-b5a1c68d76be4a47b4c4388511056e402022-12-21T18:36:04ZengNature PortfolioScientific Reports2045-23222021-01-0111111010.1038/s41598-021-81358-6A novel rabbit fixator made of a thermoplastic mask for awake imaging experimentsRencai Lu0Li Hou1Siyu Wang2Bo She3Hong He4Wentao Gao5Sidang Wang6Dongdong Xv7Yunhai Ji8Shasha Yang9Zhaohui Yang10Shaobo Wang11PET-CT Center, the First People’s Hospital of Yunnan ProvinceDepartment of Radiation, the First People’s Hospital of Yunnan ProvincePET-CT Center, the First People’s Hospital of Yunnan ProvincePET-CT Center, the First People’s Hospital of Yunnan ProvincePET-CT Center, the First People’s Hospital of Yunnan ProvincePET-CT Center, the First People’s Hospital of Yunnan ProvincePET-CT Center, the First People’s Hospital of Yunnan ProvincePET-CT Center, the First People’s Hospital of Yunnan ProvincePET-CT Center, the First People’s Hospital of Yunnan ProvincePET-CT Center, the First People’s Hospital of Yunnan ProvinceYunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and TechnologyPET-CT Center, the First People’s Hospital of Yunnan ProvinceAbstract This study aimed to develop and validate a novel rabbit fixator made from a thermoplastic mask for awake imaging experiments. When heated in a hot-water bath at 65–70 °C for 2–5 min, the thermoplastic mask became soft and could be molded to fit over the entire body of an anesthetized rabbit (4 ml of 3% pentobarbital sodium solution by intramuscular injection). Twenty rabbits were randomly divided into fixator (n = 10) and anesthesia (n = 10) groups. The animals’ vital signs, stress hormones (cortisol and adrenaline), and subjective image quality scores for the computed tomography (CT), positron emission tomography (PET), and magnetic resonance imaging (MRI) scanning were measured and compared. Phantom CT, MRI and PET studies were performed to assess the performance with and without the thermoplastic mask by using image agents at different concentrations or with different radioactivity. The respiration rate (RR), systolic blood pressure (SBP), diastolic blood pressure (DBP), peripheral capillary oxygen saturation (SpO2) and body temperature (T) decreased after anesthesia (all P < 0.05) but did not significantly decrease after fixation (all P > 0.05). The heart rate (HR), cortisol and adrenaline did not significantly decrease after either anesthesia or fixation (all P > 0.05). The subjective image quality scores for the CT and MRI images of the head, thorax, liver, kidney, intestines and pelvis and the subjective image quality scores for the PET images did not significantly differ between the two groups (all P > 0.05). For all examined organs except the muscle, 18F-FDG metabolism was lower after fixation than after anesthesia, and was almost identical of liver between two groups. The phantom study showed that the CT values, standard uptake values and MR T2 signal values did not differ significantly with or without the mask (all P > 0.05). A novel rabbit fixator created using a thermoplastic mask could be used to obtain high-quality images for different imaging modalities in an awake and near-physiological state.https://doi.org/10.1038/s41598-021-81358-6 |
| spellingShingle | Rencai Lu Li Hou Siyu Wang Bo She Hong He Wentao Gao Sidang Wang Dongdong Xv Yunhai Ji Shasha Yang Zhaohui Yang Shaobo Wang A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments Scientific Reports |
| title | A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments |
| title_full | A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments |
| title_fullStr | A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments |
| title_full_unstemmed | A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments |
| title_short | A novel rabbit fixator made of a thermoplastic mask for awake imaging experiments |
| title_sort | novel rabbit fixator made of a thermoplastic mask for awake imaging experiments |
| url | https://doi.org/10.1038/s41598-021-81358-6 |
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