The Isolation and Identification of <i>Pseudoalteromonas</i> sp. H27, a Bacterial Strain Pathogenic to <i>Crassostrea gigas</i>
Bacterial infection is frequently observed in disease outbreaks of aquatic animals, making it of significance to isolate and identify the bacterial pathogens. In this study, diseased individuals of <i>Crassostrea gigas</i> were sampled from the nearshore area in Zhanjiang, Guangdong in M...
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MDPI AG
2025-01-01
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| Serija: | Microorganisms |
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| Online pristup: | https://www.mdpi.com/2076-2607/13/2/296 |
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| author | Heyang Qin Junyi Jiang Zhikai Jing Jiayu Wang Shuang Xu Rongwei Chen Bo Wang Zhongming Huo Lei Fang |
| author_facet | Heyang Qin Junyi Jiang Zhikai Jing Jiayu Wang Shuang Xu Rongwei Chen Bo Wang Zhongming Huo Lei Fang |
| author_sort | Heyang Qin |
| collection | DOAJ |
| description | Bacterial infection is frequently observed in disease outbreaks of aquatic animals, making it of significance to isolate and identify the bacterial pathogens. In this study, diseased individuals of <i>Crassostrea gigas</i> were sampled from the nearshore area in Zhanjiang, Guangdong in May 2023. Culturable bacteria were isolated from the diseased tissue and a pathogenic strain labeled as H27 was screened through a hemolysis test and bacterial challenge experiments. Morphological characterization, 16S rRNA gene sequence-based molecular identification and biochemical tests showed that strain H27 belonged to the genus of <i>Pseudoalteromonas</i>, a dominant genus in the diseased tissue of <i>C. gigas</i> revealed by bacterial community structure analysis. The clinical signs originally observed in naturally diseased <i>C. gigas</i> were reproduced in strain H27-challenged adults, both with the red mantle and adductor. Histopathological analysis was further performed on the diseased tissues of the latter, which showed a significantly increased accumulation of pigment granules in the cytoplasm of the diseased mantle as well as enlarged muscle fiber distances in the diseased adductor. In addition, strain H27 was re-isolated from tissues of the moribund <i>C. gigas</i> after bacterial challenge, indicating the fulfillment of Koch’s postulate. Our results help to enrich the knowledge of <i>C. gigas</i> diseases, possibly contributing to disease prevention and control. |
| first_indexed | 2025-03-14T14:57:19Z |
| format | Article |
| id | doaj.art-b5a1f1f7d24b4bf2a48c75816a1567c0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2025-03-14T14:57:19Z |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b5a1f1f7d24b4bf2a48c75816a1567c02025-02-25T13:39:34ZengMDPI AGMicroorganisms2076-26072025-01-0113229610.3390/microorganisms13020296The Isolation and Identification of <i>Pseudoalteromonas</i> sp. H27, a Bacterial Strain Pathogenic to <i>Crassostrea gigas</i>Heyang Qin0Junyi Jiang1Zhikai Jing2Jiayu Wang3Shuang Xu4Rongwei Chen5Bo Wang6Zhongming Huo7Lei Fang8College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian 116023, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian 116023, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian 116023, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian 116023, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian 116023, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian 116023, ChinaCollege of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, ChinaCollege of Marine Science and Environment, Dalian Ocean University, Dalian 116023, ChinaBacterial infection is frequently observed in disease outbreaks of aquatic animals, making it of significance to isolate and identify the bacterial pathogens. In this study, diseased individuals of <i>Crassostrea gigas</i> were sampled from the nearshore area in Zhanjiang, Guangdong in May 2023. Culturable bacteria were isolated from the diseased tissue and a pathogenic strain labeled as H27 was screened through a hemolysis test and bacterial challenge experiments. Morphological characterization, 16S rRNA gene sequence-based molecular identification and biochemical tests showed that strain H27 belonged to the genus of <i>Pseudoalteromonas</i>, a dominant genus in the diseased tissue of <i>C. gigas</i> revealed by bacterial community structure analysis. The clinical signs originally observed in naturally diseased <i>C. gigas</i> were reproduced in strain H27-challenged adults, both with the red mantle and adductor. Histopathological analysis was further performed on the diseased tissues of the latter, which showed a significantly increased accumulation of pigment granules in the cytoplasm of the diseased mantle as well as enlarged muscle fiber distances in the diseased adductor. In addition, strain H27 was re-isolated from tissues of the moribund <i>C. gigas</i> after bacterial challenge, indicating the fulfillment of Koch’s postulate. Our results help to enrich the knowledge of <i>C. gigas</i> diseases, possibly contributing to disease prevention and control.https://www.mdpi.com/2076-2607/13/2/296<i>C. gigas</i>bacteriapathogenichistopathological analysisbacterial challenge experiment<i>Pseudoalteromonas</i> |
| spellingShingle | Heyang Qin Junyi Jiang Zhikai Jing Jiayu Wang Shuang Xu Rongwei Chen Bo Wang Zhongming Huo Lei Fang The Isolation and Identification of <i>Pseudoalteromonas</i> sp. H27, a Bacterial Strain Pathogenic to <i>Crassostrea gigas</i> Microorganisms <i>C. gigas</i> bacteria pathogenic histopathological analysis bacterial challenge experiment <i>Pseudoalteromonas</i> |
| title | The Isolation and Identification of <i>Pseudoalteromonas</i> sp. H27, a Bacterial Strain Pathogenic to <i>Crassostrea gigas</i> |
| title_full | The Isolation and Identification of <i>Pseudoalteromonas</i> sp. H27, a Bacterial Strain Pathogenic to <i>Crassostrea gigas</i> |
| title_fullStr | The Isolation and Identification of <i>Pseudoalteromonas</i> sp. H27, a Bacterial Strain Pathogenic to <i>Crassostrea gigas</i> |
| title_full_unstemmed | The Isolation and Identification of <i>Pseudoalteromonas</i> sp. H27, a Bacterial Strain Pathogenic to <i>Crassostrea gigas</i> |
| title_short | The Isolation and Identification of <i>Pseudoalteromonas</i> sp. H27, a Bacterial Strain Pathogenic to <i>Crassostrea gigas</i> |
| title_sort | isolation and identification of i pseudoalteromonas i sp h27 a bacterial strain pathogenic to i crassostrea gigas i |
| topic | <i>C. gigas</i> bacteria pathogenic histopathological analysis bacterial challenge experiment <i>Pseudoalteromonas</i> |
| url | https://www.mdpi.com/2076-2607/13/2/296 |
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