Protocol to evaluate hyperlipidemia in zebrafish larvae
Summary: In this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an in vivo system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722006992 |
| _version_ | 1797980835224223744 |
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| author | Keerthana Balamurugan Raghavender Medishetti Pallavi Rao Rahul Varma K Kiranam Chatti Kishore V.L. Parsa |
| author_facet | Keerthana Balamurugan Raghavender Medishetti Pallavi Rao Rahul Varma K Kiranam Chatti Kishore V.L. Parsa |
| author_sort | Keerthana Balamurugan |
| collection | DOAJ |
| description | Summary: In this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an in vivo system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, and utilization of ImageJ to evaluate larval dimensions and to explore the model in the context of hyperlipidemia. Researchers should be aware of context specificity of the proposed protocols and interpret results accordingly.For complete details on the use and execution of this protocol, please refer to Balamurugan et al., (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T06:00:07Z |
| format | Article |
| id | doaj.art-b5a51f5e18544b4a9546639a20023c5f |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T06:00:07Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-b5a51f5e18544b4a9546639a20023c5f2022-12-22T04:41:43ZengElsevierSTAR Protocols2666-16672022-12-0134101819Protocol to evaluate hyperlipidemia in zebrafish larvaeKeerthana Balamurugan0Raghavender Medishetti1Pallavi Rao2Rahul Varma K3Kiranam Chatti4Kishore V.L. Parsa5Center for Innovation in Molecular and Pharmaceutical Sciences, Dr. Reddy’s Institute of Life Sciences, Hyderabad, Telangana, India; Doctoral Student Registered with Manipal Academy of Higher Education, Manipal, Karnataka, IndiaCenter for Innovation in Molecular and Pharmaceutical Sciences, Dr. Reddy’s Institute of Life Sciences, Hyderabad, Telangana, India; Doctoral Student Registered with Manipal Academy of Higher Education, Manipal, Karnataka, IndiaCenter for Process Research and Innovation, Dr. Reddy’s Institute of Life Sciences, Hyderabad, Telangana, IndiaCenter for Process Research and Innovation, Dr. Reddy’s Institute of Life Sciences, Hyderabad, Telangana, IndiaCenter for Innovation in Molecular and Pharmaceutical Sciences, Dr. Reddy’s Institute of Life Sciences, Hyderabad, Telangana, India; Corresponding authorCenter for Innovation in Molecular and Pharmaceutical Sciences, Dr. Reddy’s Institute of Life Sciences, Hyderabad, Telangana, India; Corresponding authorSummary: In this report, we describe an approach to generate a zebrafish larval model of lipid accumulation that can be used as an in vivo system to study hyperlipidemic conditions such as atherosclerosis. Furthermore, we detail steps on staining techniques, lipid estimation assays, RNA isolation, and utilization of ImageJ to evaluate larval dimensions and to explore the model in the context of hyperlipidemia. Researchers should be aware of context specificity of the proposed protocols and interpret results accordingly.For complete details on the use and execution of this protocol, please refer to Balamurugan et al., (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722006992MetabolismModel organisms |
| spellingShingle | Keerthana Balamurugan Raghavender Medishetti Pallavi Rao Rahul Varma K Kiranam Chatti Kishore V.L. Parsa Protocol to evaluate hyperlipidemia in zebrafish larvae STAR Protocols Metabolism Model organisms |
| title | Protocol to evaluate hyperlipidemia in zebrafish larvae |
| title_full | Protocol to evaluate hyperlipidemia in zebrafish larvae |
| title_fullStr | Protocol to evaluate hyperlipidemia in zebrafish larvae |
| title_full_unstemmed | Protocol to evaluate hyperlipidemia in zebrafish larvae |
| title_short | Protocol to evaluate hyperlipidemia in zebrafish larvae |
| title_sort | protocol to evaluate hyperlipidemia in zebrafish larvae |
| topic | Metabolism Model organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722006992 |
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