Subclinical endometritis increases oxidative stress and modulates polymorphonuclear leukocyte functions in crossbred cows
Subclinical endometritis (SCE) adversely affects fertility and is a diagnostic challenge in bovine practice. In the present study, it was hypothesized that SCE in cows influence the plasma levels of nitric oxide (NO), lipid peroxide (LPO) and polymorphonuclear (PMN) cell functions. Cows with SCE (n...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2014-11-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/44736 |
| _version_ | 1797659598568554496 |
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| author | BINSILA B KRISHNAN HARENDRA KUMAR SANJEEV MEHROTRA SANJAY K SINGH TAPAS KUMAR GOSWAMI KRISHNASWAMY NARAYANAN |
| author_facet | BINSILA B KRISHNAN HARENDRA KUMAR SANJEEV MEHROTRA SANJAY K SINGH TAPAS KUMAR GOSWAMI KRISHNASWAMY NARAYANAN |
| author_sort | BINSILA B KRISHNAN |
| collection | DOAJ |
| description |
Subclinical endometritis (SCE) adversely affects fertility and is a diagnostic challenge in bovine practice. In the present study, it was hypothesized that SCE in cows influence the plasma levels of nitric oxide (NO), lipid peroxide (LPO) and polymorphonuclear (PMN) cell functions. Cows with SCE (n,12) were selected on the basis of positive colour reaction of cervico-vaginal mucus (CVM) to Whiteside test, alkaline pH of CVM and presence of > 5% PMN cells in uterine cytology smears. Cows without endometritis served as negative control (n,12). Functions of PMN cells were assessed by estimating superoxide (O2¯) and hydrogen peroxide (H2O2) production ability. Further, to assess the inflammatory status and oxidative stress, plasma levels of NO and LPO were measured. The results revealed that cows with SCE had significantly higher H2O2 (19.70±6.43 vs 2.52±0.71 nmol/2 × 106 cells/30 min incubation) in isolated blood PMN cells as compared to non endometritic cows (P<0.05). Similarly, an increased plasma concentrations of NO (81.34±1.70 vs 57.50±1.36 µmol/L) and LPO (712.00±50.39 vs 402.78±21.61 nmol MDA/L) were observed in cows with SCE. The results suggested that SCE increases oxidative stress and PMN cell functions despite being a local inflammation and may have potential in the diagnosis of SCE or monitoring the efficacy of treatment.
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| first_indexed | 2024-03-11T18:17:28Z |
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| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-03-11T18:17:28Z |
| publishDate | 2014-11-01 |
| publisher | Indian Council of Agricultural Research |
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| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-b5a5cddd744044ce88ef745728a1f70d2023-10-16T05:57:10ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272014-11-01841110.56093/ijans.v84i11.44736Subclinical endometritis increases oxidative stress and modulates polymorphonuclear leukocyte functions in crossbred cowsBINSILA B KRISHNAN0HARENDRA KUMAR1SANJEEV MEHROTRA2SANJAY K SINGH3TAPAS KUMAR GOSWAMI4KRISHNASWAMY NARAYANAN5Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaIndian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India Subclinical endometritis (SCE) adversely affects fertility and is a diagnostic challenge in bovine practice. In the present study, it was hypothesized that SCE in cows influence the plasma levels of nitric oxide (NO), lipid peroxide (LPO) and polymorphonuclear (PMN) cell functions. Cows with SCE (n,12) were selected on the basis of positive colour reaction of cervico-vaginal mucus (CVM) to Whiteside test, alkaline pH of CVM and presence of > 5% PMN cells in uterine cytology smears. Cows without endometritis served as negative control (n,12). Functions of PMN cells were assessed by estimating superoxide (O2¯) and hydrogen peroxide (H2O2) production ability. Further, to assess the inflammatory status and oxidative stress, plasma levels of NO and LPO were measured. The results revealed that cows with SCE had significantly higher H2O2 (19.70±6.43 vs 2.52±0.71 nmol/2 × 106 cells/30 min incubation) in isolated blood PMN cells as compared to non endometritic cows (P<0.05). Similarly, an increased plasma concentrations of NO (81.34±1.70 vs 57.50±1.36 µmol/L) and LPO (712.00±50.39 vs 402.78±21.61 nmol MDA/L) were observed in cows with SCE. The results suggested that SCE increases oxidative stress and PMN cell functions despite being a local inflammation and may have potential in the diagnosis of SCE or monitoring the efficacy of treatment. https://epubs.icar.org.in/index.php/IJAnS/article/view/44736CowsLipid peroxideNitric oxidePMN cell functionSubclinical endometritis |
| spellingShingle | BINSILA B KRISHNAN HARENDRA KUMAR SANJEEV MEHROTRA SANJAY K SINGH TAPAS KUMAR GOSWAMI KRISHNASWAMY NARAYANAN Subclinical endometritis increases oxidative stress and modulates polymorphonuclear leukocyte functions in crossbred cows Indian Journal of Animal Sciences Cows Lipid peroxide Nitric oxide PMN cell function Subclinical endometritis |
| title | Subclinical endometritis increases oxidative stress and modulates polymorphonuclear leukocyte functions in crossbred cows |
| title_full | Subclinical endometritis increases oxidative stress and modulates polymorphonuclear leukocyte functions in crossbred cows |
| title_fullStr | Subclinical endometritis increases oxidative stress and modulates polymorphonuclear leukocyte functions in crossbred cows |
| title_full_unstemmed | Subclinical endometritis increases oxidative stress and modulates polymorphonuclear leukocyte functions in crossbred cows |
| title_short | Subclinical endometritis increases oxidative stress and modulates polymorphonuclear leukocyte functions in crossbred cows |
| title_sort | subclinical endometritis increases oxidative stress and modulates polymorphonuclear leukocyte functions in crossbred cows |
| topic | Cows Lipid peroxide Nitric oxide PMN cell function Subclinical endometritis |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/44736 |
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