Differential Expression of CD45RO and CD45RA in Bovine T Cells
Effective vaccination induces immune memory to protect animals upon pathogen re-encounter. Despite contradictory reports, bovine memory T cells are identified based on two isoforms of CD45, expression of CD45RO plus exclusion of CD45RA. In this report, we contrasted CD45RA/RO expression on circulato...
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MDPI AG
2022-06-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/11/1844 |
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| author | Anmol Kandel Lei Li Akanksha Hada Zhengguo Xiao |
| author_facet | Anmol Kandel Lei Li Akanksha Hada Zhengguo Xiao |
| author_sort | Anmol Kandel |
| collection | DOAJ |
| description | Effective vaccination induces immune memory to protect animals upon pathogen re-encounter. Despite contradictory reports, bovine memory T cells are identified based on two isoforms of CD45, expression of CD45RO plus exclusion of CD45RA. In this report, we contrasted CD45RA/RO expression on circulatory T cells with IFNγ and IL4 expression induced by a conventional method. To our surprise, 20% of cattle from an enclosed herd did not express CD45RO on T cells without any significant difference on CD45RA expression and IFNγ or IL4 induction. In CD45RO expressing cattle, CD45RA and CD45RO expressions excluded each other, with dominant CD45RO (>90%) expression on gamma delta (γδ) followed by CD4+ (60%) but significantly higher CD45RA expression on CD8+ T cells (about 80%). Importantly, more than 80% of CD45RO expressing CD4+ and CD8+ T cells failed to produce IFNγ and IL-4; however, within the cytokine inducing cells, CD4+ T cells highly expressed CD45RO but those within CD8+ T cells mostly expressed CD45RA. Hence, CD45RO is not ubiquitously expressed in cattle, and rather than with memory phenotype, CD45RA/RO expression are more associated with distinct T cell subtypes. |
| first_indexed | 2024-03-10T01:25:05Z |
| format | Article |
| id | doaj.art-b5a739e1e272483880500c7629456f68 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T01:25:05Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-b5a739e1e272483880500c7629456f682023-11-23T13:53:41ZengMDPI AGCells2073-44092022-06-011111184410.3390/cells11111844Differential Expression of CD45RO and CD45RA in Bovine T CellsAnmol Kandel0Lei Li1Akanksha Hada2Zhengguo Xiao3Department of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USADepartment of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USADepartment of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USADepartment of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USAEffective vaccination induces immune memory to protect animals upon pathogen re-encounter. Despite contradictory reports, bovine memory T cells are identified based on two isoforms of CD45, expression of CD45RO plus exclusion of CD45RA. In this report, we contrasted CD45RA/RO expression on circulatory T cells with IFNγ and IL4 expression induced by a conventional method. To our surprise, 20% of cattle from an enclosed herd did not express CD45RO on T cells without any significant difference on CD45RA expression and IFNγ or IL4 induction. In CD45RO expressing cattle, CD45RA and CD45RO expressions excluded each other, with dominant CD45RO (>90%) expression on gamma delta (γδ) followed by CD4+ (60%) but significantly higher CD45RA expression on CD8+ T cells (about 80%). Importantly, more than 80% of CD45RO expressing CD4+ and CD8+ T cells failed to produce IFNγ and IL-4; however, within the cytokine inducing cells, CD4+ T cells highly expressed CD45RO but those within CD8+ T cells mostly expressed CD45RA. Hence, CD45RO is not ubiquitously expressed in cattle, and rather than with memory phenotype, CD45RA/RO expression are more associated with distinct T cell subtypes.https://www.mdpi.com/2073-4409/11/11/1844cattlememoryCD4+ T cellsCD8+ T cellsγδ T cellsCD45RO |
| spellingShingle | Anmol Kandel Lei Li Akanksha Hada Zhengguo Xiao Differential Expression of CD45RO and CD45RA in Bovine T Cells Cells cattle memory CD4+ T cells CD8+ T cells γδ T cells CD45RO |
| title | Differential Expression of CD45RO and CD45RA in Bovine T Cells |
| title_full | Differential Expression of CD45RO and CD45RA in Bovine T Cells |
| title_fullStr | Differential Expression of CD45RO and CD45RA in Bovine T Cells |
| title_full_unstemmed | Differential Expression of CD45RO and CD45RA in Bovine T Cells |
| title_short | Differential Expression of CD45RO and CD45RA in Bovine T Cells |
| title_sort | differential expression of cd45ro and cd45ra in bovine t cells |
| topic | cattle memory CD4+ T cells CD8+ T cells γδ T cells CD45RO |
| url | https://www.mdpi.com/2073-4409/11/11/1844 |
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