Wild <em>Vanilla planifolia</em> and its relatives in the Mexican Yucatan Peninsula: Systematic analyses with ISSR and ITS
Background: Vanilla planifolia, a threatened species, is distributed naturally in semi-deciduous and evergreen rain forests of southeastern Mexico and parts of Central America. In the wild, it is difficult to diagnose from another sympatric Vanilla species, and individuals with reproductive structu...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedad Botánica de México, A. C.
2017-07-01
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| Series: | Botanical Sciences |
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| Online Access: | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/668 |
| _version_ | 1797984155826388992 |
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| author | Sara Villanueva-Viramontes Mariana Hernández-Apolinar Germán Carnevali Fernández-Concha Alfredo Dorantes-Euán Gabriel R. Dzib Jaime Martínez Castillo |
| author_facet | Sara Villanueva-Viramontes Mariana Hernández-Apolinar Germán Carnevali Fernández-Concha Alfredo Dorantes-Euán Gabriel R. Dzib Jaime Martínez Castillo |
| author_sort | Sara Villanueva-Viramontes |
| collection | DOAJ |
| description |
Background: Vanilla planifolia, a threatened species, is distributed naturally in semi-deciduous and evergreen rain forests of southeastern Mexico and parts of Central America. In the wild, it is difficult to diagnose from another sympatric Vanilla species, and individuals with reproductive structures are usually required.
Questions: Can ISSR discriminate wild individuals of Vanilla planifolia from other sympatric species of the genus? Can phylogenetic analyses of ITS recover the monophyly of V. planifolia and help identify Vanilla species?
Studied species: The vanilla (V. planifolia), the second-most important economically spice in the world.
Study site and years of study: Plant material was collected during 2014 in the Mexican Yucatan Peninsula.
Methods: We analyzed 88 wild individuals of several Vanilla species. Individuals of five species of Vanilla reported for the region were used as reference. 84 ISSR loci were analyzed using three clustering methods. A phylogenetic inference using ITS was performed.
Results: ISSR markers clearly discriminate wild Vanilla planifolia, finding definite genetic structure within the species. The three clustering methods identified genetic relationships with congruent patterns. Five groups were found and they corresponded with the species studied. Phylogenetic inference of ITS sequences supported the monophyly of the Vanilla and the resulting cladograms were coherent with the clustering pattern observed in the ISSR studies.
Conclusions: Both ISSR and ITS analyses are able to identify V. planifolia. Molecular data suggest the presence of V. pompona and a new species of Vanilla in the Yucatan Peninsula.
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| first_indexed | 2024-04-11T06:57:06Z |
| format | Article |
| id | doaj.art-b5a7605c1a634a15828b75891b516c56 |
| institution | Directory Open Access Journal |
| issn | 2007-4298 2007-4476 |
| language | English |
| last_indexed | 2024-04-11T06:57:06Z |
| publishDate | 2017-07-01 |
| publisher | Sociedad Botánica de México, A. C. |
| record_format | Article |
| series | Botanical Sciences |
| spelling | doaj.art-b5a7605c1a634a15828b75891b516c562022-12-22T04:38:59ZengSociedad Botánica de México, A. C.Botanical Sciences2007-42982007-44762017-07-0195210.17129/botsci.668Wild <em>Vanilla planifolia</em> and its relatives in the Mexican Yucatan Peninsula: Systematic analyses with ISSR and ITSSara Villanueva-ViramontesMariana Hernández-ApolinarGermán Carnevali Fernández-ConchaAlfredo Dorantes-EuánGabriel R. DzibJaime Martínez Castillo Background: Vanilla planifolia, a threatened species, is distributed naturally in semi-deciduous and evergreen rain forests of southeastern Mexico and parts of Central America. In the wild, it is difficult to diagnose from another sympatric Vanilla species, and individuals with reproductive structures are usually required. Questions: Can ISSR discriminate wild individuals of Vanilla planifolia from other sympatric species of the genus? Can phylogenetic analyses of ITS recover the monophyly of V. planifolia and help identify Vanilla species? Studied species: The vanilla (V. planifolia), the second-most important economically spice in the world. Study site and years of study: Plant material was collected during 2014 in the Mexican Yucatan Peninsula. Methods: We analyzed 88 wild individuals of several Vanilla species. Individuals of five species of Vanilla reported for the region were used as reference. 84 ISSR loci were analyzed using three clustering methods. A phylogenetic inference using ITS was performed. Results: ISSR markers clearly discriminate wild Vanilla planifolia, finding definite genetic structure within the species. The three clustering methods identified genetic relationships with congruent patterns. Five groups were found and they corresponded with the species studied. Phylogenetic inference of ITS sequences supported the monophyly of the Vanilla and the resulting cladograms were coherent with the clustering pattern observed in the ISSR studies. Conclusions: Both ISSR and ITS analyses are able to identify V. planifolia. Molecular data suggest the presence of V. pompona and a new species of Vanilla in the Yucatan Peninsula. https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/668Genetic identificationmolecular markersphylogenetic analysisVanillawild populations |
| spellingShingle | Sara Villanueva-Viramontes Mariana Hernández-Apolinar Germán Carnevali Fernández-Concha Alfredo Dorantes-Euán Gabriel R. Dzib Jaime Martínez Castillo Wild <em>Vanilla planifolia</em> and its relatives in the Mexican Yucatan Peninsula: Systematic analyses with ISSR and ITS Botanical Sciences Genetic identification molecular markers phylogenetic analysis Vanilla wild populations |
| title | Wild <em>Vanilla planifolia</em> and its relatives in the Mexican Yucatan Peninsula: Systematic analyses with ISSR and ITS |
| title_full | Wild <em>Vanilla planifolia</em> and its relatives in the Mexican Yucatan Peninsula: Systematic analyses with ISSR and ITS |
| title_fullStr | Wild <em>Vanilla planifolia</em> and its relatives in the Mexican Yucatan Peninsula: Systematic analyses with ISSR and ITS |
| title_full_unstemmed | Wild <em>Vanilla planifolia</em> and its relatives in the Mexican Yucatan Peninsula: Systematic analyses with ISSR and ITS |
| title_short | Wild <em>Vanilla planifolia</em> and its relatives in the Mexican Yucatan Peninsula: Systematic analyses with ISSR and ITS |
| title_sort | wild em vanilla planifolia em and its relatives in the mexican yucatan peninsula systematic analyses with issr and its |
| topic | Genetic identification molecular markers phylogenetic analysis Vanilla wild populations |
| url | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/668 |
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