The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded Mice
It is well known that physical inactivity during space flight or prolonged bed rest causes rapid bone loss. Soy isoflavones (ISOs) and resveratrol (RES) have been reported to be useful to maintain a positive balance for bone turnover. Therefore, we examined the combined effects of ISO and RES on bon...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-07-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/12/7/2043 |
| _version_ | 1797562951042859008 |
|---|---|
| author | Yuko Tousen Ryota Ichimaru Takashi Kondo Masaki Inada Chisato Miyaura Yoshiko Ishimi |
| author_facet | Yuko Tousen Ryota Ichimaru Takashi Kondo Masaki Inada Chisato Miyaura Yoshiko Ishimi |
| author_sort | Yuko Tousen |
| collection | DOAJ |
| description | It is well known that physical inactivity during space flight or prolonged bed rest causes rapid bone loss. Soy isoflavones (ISOs) and resveratrol (RES) have been reported to be useful to maintain a positive balance for bone turnover. Therefore, we examined the combined effects of ISO and RES on bone loss that was induced by hindlimb-unloading in mice. Female eight-week-old ddY mice were divided into the following six groups (<i>n</i> = 6–8 each): normally housed mice, loading mice, hindlimb-unloading (UL) mice fed a control diet, UL mice fed a 0.16% ISO conjugates, UL mice fed a 0.15% RES diet, and UL mice fed a 0.16% ISO and 0.15% RES diet. After three weeks, femoral bone mineral density was markedly decreased in unloading mice. The combination of ISO and RES prevented bone loss and especially maintained the trabecular bone mineral density more effectively compared with cortical bones. ISO and/or RES inhibited the increase in the RANKL/OPG expression ratio in bone marrow cells in UL mice. These results suggest that the combination of ISO and RES had a preventive effect against bone loss induced by hindlimb-unloading in mice. These osteoprotective effects of ISO and RES may result from the inhibition of bone resorption. |
| first_indexed | 2024-03-10T18:35:53Z |
| format | Article |
| id | doaj.art-b5a7d58992b549689f69e3307ca787bb |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T18:35:53Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-b5a7d58992b549689f69e3307ca787bb2023-11-20T06:17:50ZengMDPI AGNutrients2072-66432020-07-01127204310.3390/nu12072043The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded MiceYuko Tousen0Ryota Ichimaru1Takashi Kondo2Masaki Inada3Chisato Miyaura4Yoshiko Ishimi5Department of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, JapanDepartment of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, JapanDepartment of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, JapanCooperative Major in Advanced Health Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei, Tokyo 184-8588, Japan.Cooperative Major in Advanced Health Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei, Tokyo 184-8588, Japan.Department of Food Function and Labeling, National Institute of Health and Nutrition, National Institutes of Biomedical Innovation, Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636, JapanIt is well known that physical inactivity during space flight or prolonged bed rest causes rapid bone loss. Soy isoflavones (ISOs) and resveratrol (RES) have been reported to be useful to maintain a positive balance for bone turnover. Therefore, we examined the combined effects of ISO and RES on bone loss that was induced by hindlimb-unloading in mice. Female eight-week-old ddY mice were divided into the following six groups (<i>n</i> = 6–8 each): normally housed mice, loading mice, hindlimb-unloading (UL) mice fed a control diet, UL mice fed a 0.16% ISO conjugates, UL mice fed a 0.15% RES diet, and UL mice fed a 0.16% ISO and 0.15% RES diet. After three weeks, femoral bone mineral density was markedly decreased in unloading mice. The combination of ISO and RES prevented bone loss and especially maintained the trabecular bone mineral density more effectively compared with cortical bones. ISO and/or RES inhibited the increase in the RANKL/OPG expression ratio in bone marrow cells in UL mice. These results suggest that the combination of ISO and RES had a preventive effect against bone loss induced by hindlimb-unloading in mice. These osteoprotective effects of ISO and RES may result from the inhibition of bone resorption.https://www.mdpi.com/2072-6643/12/7/2043hindlimb-unloadingbone mineral densitysoy isoflavonesresveratrolbone strength |
| spellingShingle | Yuko Tousen Ryota Ichimaru Takashi Kondo Masaki Inada Chisato Miyaura Yoshiko Ishimi The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded Mice Nutrients hindlimb-unloading bone mineral density soy isoflavones resveratrol bone strength |
| title | The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded Mice |
| title_full | The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded Mice |
| title_fullStr | The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded Mice |
| title_full_unstemmed | The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded Mice |
| title_short | The Combination of Soy Isoflavones and Resveratrol Preserve Bone Mineral Density in Hindlimb-Unloaded Mice |
| title_sort | combination of soy isoflavones and resveratrol preserve bone mineral density in hindlimb unloaded mice |
| topic | hindlimb-unloading bone mineral density soy isoflavones resveratrol bone strength |
| url | https://www.mdpi.com/2072-6643/12/7/2043 |
| work_keys_str_mv | AT yukotousen thecombinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT ryotaichimaru thecombinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT takashikondo thecombinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT masakiinada thecombinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT chisatomiyaura thecombinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT yoshikoishimi thecombinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT yukotousen combinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT ryotaichimaru combinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT takashikondo combinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT masakiinada combinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT chisatomiyaura combinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice AT yoshikoishimi combinationofsoyisoflavonesandresveratrolpreservebonemineraldensityinhindlimbunloadedmice |