The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells
Resveratrol has anti-inflammatory, anti-cancer, and anti-aging pharmacological activities. There is currently a gap in academic research regarding the uptake, transport, and reduction of H<sub>2</sub>O<sub>2</sub>-induced oxidative damage of resveratrol in the Caco-2 cell mod...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/28/12/4569 |
| _version_ | 1797593332909604864 |
|---|---|
| author | Zhen-Dong Zhang Qi Tao Li-Xia Bai Zhe Qin Xi-Wang Liu Shi-Hong Li Ya-Jun Yang Wen-Bo Ge Jian-Yong Li |
| author_facet | Zhen-Dong Zhang Qi Tao Li-Xia Bai Zhe Qin Xi-Wang Liu Shi-Hong Li Ya-Jun Yang Wen-Bo Ge Jian-Yong Li |
| author_sort | Zhen-Dong Zhang |
| collection | DOAJ |
| description | Resveratrol has anti-inflammatory, anti-cancer, and anti-aging pharmacological activities. There is currently a gap in academic research regarding the uptake, transport, and reduction of H<sub>2</sub>O<sub>2</sub>-induced oxidative damage of resveratrol in the Caco-2 cell model. This study investigated the role of resveratrol in the uptake, transport, and alleviation of H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in Caco-2 cells. In the Caco-2 cell transport model, it was observed that the uptake and transport of resveratrol (10, 20, 40, and 80 μM) were time dependent and concentration dependent. Different temperatures (37 °C vs. 4 °C) could significantly affect the uptake and transportation of resveratrol. The apical to basolateral transport of resveratrol was markedly reduced by STF-31, a GLUT1 inhibitor, and siRNA intervention. Furthermore, resveratrol pretreatment (80 μM) improves the viability of Caco-2 cells induced by H<sub>2</sub>O<sub>2</sub>. In a cellular metabolite analysis combined with ultra-high performance liquid chromatography-tandem mass spectrometry, 21 metabolites were identified as differentials. These differential metabolites belong to the urea cycle, arginine and proline metabolism, glycine and serine metabolism, ammonia recycling, aspartate metabolism, glutathione metabolism, and other metabolic pathways. The transport, uptake, and metabolism of resveratrol suggest that oral resveratrol could prevent intestinal diseases caused by oxidative stress. |
| first_indexed | 2024-03-11T02:07:39Z |
| format | Article |
| id | doaj.art-b5a9a9379d6d4d84888a0a4b214c3c45 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T02:07:39Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-b5a9a9379d6d4d84888a0a4b214c3c452023-11-18T11:47:24ZengMDPI AGMolecules1420-30492023-06-012812456910.3390/molecules28124569The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 CellsZhen-Dong Zhang0Qi Tao1Li-Xia Bai2Zhe Qin3Xi-Wang Liu4Shi-Hong Li5Ya-Jun Yang6Wen-Bo Ge7Jian-Yong Li8Key Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, ChinaKey Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, ChinaKey Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, ChinaKey Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, ChinaKey Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, ChinaKey Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, ChinaKey Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, ChinaKey Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, ChinaKey Lab of New Animal Drug Project of Gansu Province, Key Lab of Veterinary Pharmaceutical Development of Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, ChinaResveratrol has anti-inflammatory, anti-cancer, and anti-aging pharmacological activities. There is currently a gap in academic research regarding the uptake, transport, and reduction of H<sub>2</sub>O<sub>2</sub>-induced oxidative damage of resveratrol in the Caco-2 cell model. This study investigated the role of resveratrol in the uptake, transport, and alleviation of H<sub>2</sub>O<sub>2</sub>-induced oxidative damage in Caco-2 cells. In the Caco-2 cell transport model, it was observed that the uptake and transport of resveratrol (10, 20, 40, and 80 μM) were time dependent and concentration dependent. Different temperatures (37 °C vs. 4 °C) could significantly affect the uptake and transportation of resveratrol. The apical to basolateral transport of resveratrol was markedly reduced by STF-31, a GLUT1 inhibitor, and siRNA intervention. Furthermore, resveratrol pretreatment (80 μM) improves the viability of Caco-2 cells induced by H<sub>2</sub>O<sub>2</sub>. In a cellular metabolite analysis combined with ultra-high performance liquid chromatography-tandem mass spectrometry, 21 metabolites were identified as differentials. These differential metabolites belong to the urea cycle, arginine and proline metabolism, glycine and serine metabolism, ammonia recycling, aspartate metabolism, glutathione metabolism, and other metabolic pathways. The transport, uptake, and metabolism of resveratrol suggest that oral resveratrol could prevent intestinal diseases caused by oxidative stress.https://www.mdpi.com/1420-3049/28/12/4569resveratrolCaco-2 celluptaketransportmetabolomics |
| spellingShingle | Zhen-Dong Zhang Qi Tao Li-Xia Bai Zhe Qin Xi-Wang Liu Shi-Hong Li Ya-Jun Yang Wen-Bo Ge Jian-Yong Li The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells Molecules resveratrol Caco-2 cell uptake transport metabolomics |
| title | The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells |
| title_full | The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells |
| title_fullStr | The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells |
| title_full_unstemmed | The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells |
| title_short | The Transport and Uptake of Resveratrol Mediated via Glucose Transporter 1 and Its Antioxidant Effect in Caco-2 Cells |
| title_sort | transport and uptake of resveratrol mediated via glucose transporter 1 and its antioxidant effect in caco 2 cells |
| topic | resveratrol Caco-2 cell uptake transport metabolomics |
| url | https://www.mdpi.com/1420-3049/28/12/4569 |
| work_keys_str_mv | AT zhendongzhang thetransportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT qitao thetransportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT lixiabai thetransportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT zheqin thetransportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT xiwangliu thetransportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT shihongli thetransportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT yajunyang thetransportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT wenboge thetransportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT jianyongli thetransportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT zhendongzhang transportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT qitao transportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT lixiabai transportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT zheqin transportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT xiwangliu transportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT shihongli transportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT yajunyang transportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT wenboge transportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells AT jianyongli transportanduptakeofresveratrolmediatedviaglucosetransporter1anditsantioxidanteffectincaco2cells |