The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats
Diazinon is an organophosphate pesticide often used by farmer. The residue of diazinon may contaminate agricultural products and if accidentally consumed in a long term, may potentially led to health problems such as kidney disorders. Shallot (Allium cepa) peel was recently proposed to help recover...
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| Format: | Article |
| Language: | English |
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University of Jember, Faculty of Medicine
2022-06-01
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| Series: | Journal of Agromedicine and Medical Sciences (AMS) |
| Online Access: | https://jurnal.unej.ac.id/index.php/JAMS/article/view/27424 |
| _version_ | 1811173113125142528 |
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| author | Mukhammad Arif Hadi Khoiruddin Dina Helianti Muhammad Hasan Rosita Dewi Sheilla Rachmania |
| author_facet | Mukhammad Arif Hadi Khoiruddin Dina Helianti Muhammad Hasan Rosita Dewi Sheilla Rachmania |
| author_sort | Mukhammad Arif Hadi Khoiruddin |
| collection | DOAJ |
| description | Diazinon is an organophosphate pesticide often used by farmer. The residue of diazinon may contaminate agricultural products and if accidentally consumed in a long term, may potentially led to health problems such as kidney disorders. Shallot (Allium cepa) peel was recently proposed to help recover the kidney damage due to its high flavonoid antioxidant content. This study aims to determine the antioxidant effect of shallot peel extract in reducing oxidative stress caused by diazinon with malondialdehyde (MDA) level indicator on the kidney of Wistar rats (Rattus norvegicus). This research was a true experimental with an in-vivo posttest-only control group design. The 28 rats were divided using a simple random sampling technique. The groups were the control group (K0), the diazinon group (K1), SPEE (Shallot Peel Ethanol Extract) groups which are P1 (300 mg/kg BW), P2 (600 mg/kg BW), P3 (900 mg/kg BW), P4 (1200 mg/kg BW) and P5 (2400 mg/kg BW). At the end of the study, the MDA kidney levels was analysed using the TBARS method. Effective doses were found in 600 mg/kg BW/day, 900 mg/kg BW/day, 1200 mg/kg BW/day, and 2400 mg/kg BW/day. The optimal dose of shallot peel extract in this study was 600 mg/kg BW/day. This study showed the effectiveness of shallot peel extract in reducing kidney MDA levels in diazinon-induced Wistar rats.
Keywords: shallot peel, diazinon, kidney MDA level |
| first_indexed | 2024-04-10T17:41:25Z |
| format | Article |
| id | doaj.art-b5ab5ff9dda5488295facbfa9f449105 |
| institution | Directory Open Access Journal |
| issn | 2460-9048 2714-5654 |
| language | English |
| last_indexed | 2024-04-10T17:41:25Z |
| publishDate | 2022-06-01 |
| publisher | University of Jember, Faculty of Medicine |
| record_format | Article |
| series | Journal of Agromedicine and Medical Sciences (AMS) |
| spelling | doaj.art-b5ab5ff9dda5488295facbfa9f4491052023-02-03T12:24:20ZengUniversity of Jember, Faculty of MedicineJournal of Agromedicine and Medical Sciences (AMS)2460-90482714-56542022-06-0182727710.19184/ams.v8i2.2742427424The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar RatsMukhammad Arif Hadi Khoiruddin0Dina Helianti1Muhammad Hasan2Rosita Dewi3Sheilla Rachmania4Faculty of Medicine, University of Jember, Jember, IndonesiaDepartment of Histology, Faculty of Medicine, University of Jember, Jember, IndonesiaDepartment of Anatomy, Faculty of Medicine, University of Jember, Jember, IndonesiaDepartment of Histology, Faculty of Medicine, University of Jember, Jember, IndonesiaDepartment of Histology, Faculty of Medicine, University of Jember, Jember, IndonesiaDiazinon is an organophosphate pesticide often used by farmer. The residue of diazinon may contaminate agricultural products and if accidentally consumed in a long term, may potentially led to health problems such as kidney disorders. Shallot (Allium cepa) peel was recently proposed to help recover the kidney damage due to its high flavonoid antioxidant content. This study aims to determine the antioxidant effect of shallot peel extract in reducing oxidative stress caused by diazinon with malondialdehyde (MDA) level indicator on the kidney of Wistar rats (Rattus norvegicus). This research was a true experimental with an in-vivo posttest-only control group design. The 28 rats were divided using a simple random sampling technique. The groups were the control group (K0), the diazinon group (K1), SPEE (Shallot Peel Ethanol Extract) groups which are P1 (300 mg/kg BW), P2 (600 mg/kg BW), P3 (900 mg/kg BW), P4 (1200 mg/kg BW) and P5 (2400 mg/kg BW). At the end of the study, the MDA kidney levels was analysed using the TBARS method. Effective doses were found in 600 mg/kg BW/day, 900 mg/kg BW/day, 1200 mg/kg BW/day, and 2400 mg/kg BW/day. The optimal dose of shallot peel extract in this study was 600 mg/kg BW/day. This study showed the effectiveness of shallot peel extract in reducing kidney MDA levels in diazinon-induced Wistar rats. Keywords: shallot peel, diazinon, kidney MDA levelhttps://jurnal.unej.ac.id/index.php/JAMS/article/view/27424 |
| spellingShingle | Mukhammad Arif Hadi Khoiruddin Dina Helianti Muhammad Hasan Rosita Dewi Sheilla Rachmania The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats Journal of Agromedicine and Medical Sciences (AMS) |
| title | The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats |
| title_full | The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats |
| title_fullStr | The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats |
| title_full_unstemmed | The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats |
| title_short | The Effect of Shallot Peel Ethanol Extract in Reducing Kidney MDA Levels in Diazinon-induced Wistar Rats |
| title_sort | effect of shallot peel ethanol extract in reducing kidney mda levels in diazinon induced wistar rats |
| url | https://jurnal.unej.ac.id/index.php/JAMS/article/view/27424 |
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