The association of common glutathione S-transferases polymorphisms with inflammatory and multiorgan impairment biomarkers in COVID-19
Introduction: Due to the established role of oxidative stress in the pathophysiology of COVID-19, it has been proposed that inter-individual differences in patients' clinical manifestations might be affected by variations in genes encoding antioxidant enzymes, such as glutathione S-transferases...
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| Format: | Article |
| Language: | English |
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University of Belgrade, Medical Faculty
2023-01-01
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| Series: | Medicinski Podmladak |
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| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2023/0369-15272301051K.pdf |
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| author | Krunić Ana Jerotić Đurđa Matić Marija |
| author_facet | Krunić Ana Jerotić Đurđa Matić Marija |
| author_sort | Krunić Ana |
| collection | DOAJ |
| description | Introduction: Due to the established role of oxidative stress in the pathophysiology of COVID-19, it has been proposed that inter-individual differences in patients' clinical manifestations might be affected by variations in genes encoding antioxidant enzymes, such as glutathione S-transferases (GSTs). Aim: The aim of this study was to assess the association of polymorphisms in cytosolic GSTs (GSTA1 rs3957357, GSTM3 rs1332018 and GSTP1 rs1695) with inflammatory parameters (leukocytes, lymphocytes, monocytes, C-reactive protein (CRP), fibrinogen, ferritin) and multiorgan impairment biomarkers (urea, creatinine, AST, ALT, LDH) in COVID-19 patients at two-time points. Material and methods: GSTM3, GSTA1 and GSTP1 genotypes were determined in 150 COVID-19 patients by appropriate polymerase chain reaction (PCR) methods. Results: Inflammatory biomarkers (leukocytes, lymphocytes, monocytes) increased 7 days upon admission to the hospital (p < 0.001), while CRP and fibrinogen decreased (p < 0.001). Out of five analyzed multiorgan impairment biomarkers, only urea increased significantly 7 days upon admission (p < 0.007), while AST showed a statistically significant drop (p < 0.001). COVID-19 patients homozygous for variant GSTM3*C/C genotype had increased levels of inflammatory biomarkers such as CRP, fibrinogen and ferritin, but the borderline significance was observed only for fibrinogen (p = 0.057). COVID-19 patients homozygous for variant GSTM3*C allele had the highest levels of ALT (p = 0.021) and LDH (p = 0.045) upon admission. Conclusion: Our results on the association between GSTM3 variant genotype with parameters of systemic inflammation and liver damage in COVID-19 patients can contribute to further understanding of pathophysiological mechanisms underpinning this disease, as well as early recognition of COVID-19 patients prone to worse course of the disease. |
| first_indexed | 2024-03-13T05:55:08Z |
| format | Article |
| id | doaj.art-b5adb86c7a60471595248e8c1f029b6a |
| institution | Directory Open Access Journal |
| issn | 0369-1527 2466-5525 |
| language | English |
| last_indexed | 2024-03-13T05:55:08Z |
| publishDate | 2023-01-01 |
| publisher | University of Belgrade, Medical Faculty |
| record_format | Article |
| series | Medicinski Podmladak |
| spelling | doaj.art-b5adb86c7a60471595248e8c1f029b6a2023-06-13T06:57:43ZengUniversity of Belgrade, Medical FacultyMedicinski Podmladak0369-15272466-55252023-01-0174151580369-15272301051KThe association of common glutathione S-transferases polymorphisms with inflammatory and multiorgan impairment biomarkers in COVID-19Krunić Ana0Jerotić Đurđa1https://orcid.org/0000-0002-1664-6557Matić Marija2https://orcid.org/0000-0002-1488-3647Univerzitet u Beogradu, Medicinski fakultet, Beograd, SerbiaUniverzitet u Beogradu, Medicinski fakultet, Beograd, SerbiaUniverzitet u Beogradu, Medicinski fakultet, Beograd, SerbiaIntroduction: Due to the established role of oxidative stress in the pathophysiology of COVID-19, it has been proposed that inter-individual differences in patients' clinical manifestations might be affected by variations in genes encoding antioxidant enzymes, such as glutathione S-transferases (GSTs). Aim: The aim of this study was to assess the association of polymorphisms in cytosolic GSTs (GSTA1 rs3957357, GSTM3 rs1332018 and GSTP1 rs1695) with inflammatory parameters (leukocytes, lymphocytes, monocytes, C-reactive protein (CRP), fibrinogen, ferritin) and multiorgan impairment biomarkers (urea, creatinine, AST, ALT, LDH) in COVID-19 patients at two-time points. Material and methods: GSTM3, GSTA1 and GSTP1 genotypes were determined in 150 COVID-19 patients by appropriate polymerase chain reaction (PCR) methods. Results: Inflammatory biomarkers (leukocytes, lymphocytes, monocytes) increased 7 days upon admission to the hospital (p < 0.001), while CRP and fibrinogen decreased (p < 0.001). Out of five analyzed multiorgan impairment biomarkers, only urea increased significantly 7 days upon admission (p < 0.007), while AST showed a statistically significant drop (p < 0.001). COVID-19 patients homozygous for variant GSTM3*C/C genotype had increased levels of inflammatory biomarkers such as CRP, fibrinogen and ferritin, but the borderline significance was observed only for fibrinogen (p = 0.057). COVID-19 patients homozygous for variant GSTM3*C allele had the highest levels of ALT (p = 0.021) and LDH (p = 0.045) upon admission. Conclusion: Our results on the association between GSTM3 variant genotype with parameters of systemic inflammation and liver damage in COVID-19 patients can contribute to further understanding of pathophysiological mechanisms underpinning this disease, as well as early recognition of COVID-19 patients prone to worse course of the disease.https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2023/0369-15272301051K.pdfcovid-19oxidative stressglutathione s-transferasespolymorphism |
| spellingShingle | Krunić Ana Jerotić Đurđa Matić Marija The association of common glutathione S-transferases polymorphisms with inflammatory and multiorgan impairment biomarkers in COVID-19 Medicinski Podmladak covid-19 oxidative stress glutathione s-transferases polymorphism |
| title | The association of common glutathione S-transferases polymorphisms with inflammatory and multiorgan impairment biomarkers in COVID-19 |
| title_full | The association of common glutathione S-transferases polymorphisms with inflammatory and multiorgan impairment biomarkers in COVID-19 |
| title_fullStr | The association of common glutathione S-transferases polymorphisms with inflammatory and multiorgan impairment biomarkers in COVID-19 |
| title_full_unstemmed | The association of common glutathione S-transferases polymorphisms with inflammatory and multiorgan impairment biomarkers in COVID-19 |
| title_short | The association of common glutathione S-transferases polymorphisms with inflammatory and multiorgan impairment biomarkers in COVID-19 |
| title_sort | association of common glutathione s transferases polymorphisms with inflammatory and multiorgan impairment biomarkers in covid 19 |
| topic | covid-19 oxidative stress glutathione s-transferases polymorphism |
| url | https://scindeks-clanci.ceon.rs/data/pdf/0369-1527/2023/0369-15272301051K.pdf |
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