Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat
Abstract Introduction: This study was designed to investigate the effects of granulocyte colony-stimulating factor (G-CSF) administration in rats for 6 weeks after traumatic brain injury (TBI). Methods: Adult male Wistar rats (n = 30) were injured with controlled cortical impact device and divid...
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2010-01-01
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| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.tums.ac.ir/browse.php?a_code=A-10-1-25&slc_lang=en&sid=1 |
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| author | Mohsen Marzban Mehrdad Bakhtiary Mehdi Mehdizadeh Mohamad Taghi Joghataei Samideh Khoei Vahid Pirhajati Mahabadi Asghar Ebrahimi |
| author_facet | Mohsen Marzban Mehrdad Bakhtiary Mehdi Mehdizadeh Mohamad Taghi Joghataei Samideh Khoei Vahid Pirhajati Mahabadi Asghar Ebrahimi |
| author_sort | Mohsen Marzban |
| collection | DOAJ |
| description | Abstract Introduction: This study was designed to investigate the effects of granulocyte colony-stimulating factor (G-CSF) administration in rats for 6 weeks after traumatic brain injury (TBI). Methods: Adult male Wistar rats (n = 30) were injured with controlled cortical impact device and divided into four groups. The treatment groups (n = 10 each) were injected subcutaneously with recombinant human G-CSF. Vehicle group (n=10) received phosphate buffered saline (PBS) and only Brdu intraperitoneally. Bromodeoxyuridine (BrdU) was used for mitotic labeling. Experimental rats were injected intraperitoneally with BrdU. Rats were killed at 6th week after traumatic brain injury. Neurological functional evaluation of animals was performed before and after injury using neurological severity scores (NSS). Animals were sacrificed 42 days after TBI and brain sections were stained using Brdu immunohistochemistry. Results: Statistically significant improvement in functional outcome was observed in treatment groups when compared with control (p<0.01). This benefit was visible 7 days after TBI and persisted until 42 days (end of trial). Histological analysis showed that Brdu cell positive was more in the lesion boundary zone at treatment animal group than all injected animals. Discussion: We believe that G-CSF therapeutic protocol reported here represents an attractive strategy for the development of a clinically significant noninvasive traumatic brain injury therapy. |
| first_indexed | 2024-03-07T17:15:08Z |
| format | Article |
| id | doaj.art-b5aedc0340a04a19ad5b3f3ab22c2374 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T17:15:08Z |
| publishDate | 2010-01-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-b5aedc0340a04a19ad5b3f3ab22c23742024-03-02T23:28:20ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422010-01-01123742Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in ratMohsen MarzbanMehrdad BakhtiaryMehdi MehdizadehMohamad Taghi JoghataeiSamideh KhoeiVahid Pirhajati MahabadiAsghar EbrahimiAbstract Introduction: This study was designed to investigate the effects of granulocyte colony-stimulating factor (G-CSF) administration in rats for 6 weeks after traumatic brain injury (TBI). Methods: Adult male Wistar rats (n = 30) were injured with controlled cortical impact device and divided into four groups. The treatment groups (n = 10 each) were injected subcutaneously with recombinant human G-CSF. Vehicle group (n=10) received phosphate buffered saline (PBS) and only Brdu intraperitoneally. Bromodeoxyuridine (BrdU) was used for mitotic labeling. Experimental rats were injected intraperitoneally with BrdU. Rats were killed at 6th week after traumatic brain injury. Neurological functional evaluation of animals was performed before and after injury using neurological severity scores (NSS). Animals were sacrificed 42 days after TBI and brain sections were stained using Brdu immunohistochemistry. Results: Statistically significant improvement in functional outcome was observed in treatment groups when compared with control (p<0.01). This benefit was visible 7 days after TBI and persisted until 42 days (end of trial). Histological analysis showed that Brdu cell positive was more in the lesion boundary zone at treatment animal group than all injected animals. Discussion: We believe that G-CSF therapeutic protocol reported here represents an attractive strategy for the development of a clinically significant noninvasive traumatic brain injury therapy.http://bcn.tums.ac.ir/browse.php?a_code=A-10-1-25&slc_lang=en&sid=1Granulocyte colony-stimulating factorBromodeoxyuridineRat |
| spellingShingle | Mohsen Marzban Mehrdad Bakhtiary Mehdi Mehdizadeh Mohamad Taghi Joghataei Samideh Khoei Vahid Pirhajati Mahabadi Asghar Ebrahimi Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat Basic and Clinical Neuroscience Granulocyte colony-stimulating factor Bromodeoxyuridine Rat |
| title | Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat |
| title_full | Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat |
| title_fullStr | Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat |
| title_full_unstemmed | Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat |
| title_short | Mobilization of stem cell with granulocyte-colony stimulating factor promotes recovery after traumatic brain injury in rat |
| title_sort | mobilization of stem cell with granulocyte colony stimulating factor promotes recovery after traumatic brain injury in rat |
| topic | Granulocyte colony-stimulating factor Bromodeoxyuridine Rat |
| url | http://bcn.tums.ac.ir/browse.php?a_code=A-10-1-25&slc_lang=en&sid=1 |
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