Genetic Contribution to Variation in Blood Calcium, Phosphorus, and Alkaline Phosphatase Activity in Pigs
Blood values of calcium (Ca), inorganic phosphorus (IP), and alkaline phosphatase activity (ALP) are valuable indicators for mineral status and bone mineralization. The mineral homeostasis is maintained by absorption, retention, and excretion processes employing a number of known and unknown sensing...
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Frontiers Media S.A.
2019-06-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2019.00590/full |
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| author | Henry Reyer Michael Oster Dörte Wittenburg Eduard Murani Siriluck Ponsuksili Klaus Wimmers Klaus Wimmers |
| author_facet | Henry Reyer Michael Oster Dörte Wittenburg Eduard Murani Siriluck Ponsuksili Klaus Wimmers Klaus Wimmers |
| author_sort | Henry Reyer |
| collection | DOAJ |
| description | Blood values of calcium (Ca), inorganic phosphorus (IP), and alkaline phosphatase activity (ALP) are valuable indicators for mineral status and bone mineralization. The mineral homeostasis is maintained by absorption, retention, and excretion processes employing a number of known and unknown sensing and regulating factors with implications on immunity. Due to the high inter-individual variation of Ca and P levels in the blood of pigs and to clarify molecular contributions to this variation, the genetics of hematological traits related to the Ca and P balance were investigated in a German Landrace population, integrating both single-locus and multi-locus genome-wide association study (GWAS) approaches. Genomic heritability estimates suggest a moderate genetic contribution to the variation of hematological Ca (N = 456), IP (N = 1049), ALP (N = 439), and the Ca/P ratio (N = 455), with values ranging from 0.27 to 0.54. The genome-wide analysis of markers adds a number of genomic regions to the list of quantitative trait loci, some of which overlap with previous results. Despite the gaps in knowledge of genes involved in Ca and P metabolism, genes like THBS2, SHH, PTPRT, PTGS1, and FRAS1 with reported connections to bone metabolism were derived from the significantly associated genomic regions. Additionally, genomic regions included TRAFD1 and genes coding for phosphate transporters (SLC17A1–SLC17A4), which are linked to Ca and P homeostasis. The study calls for improved functional annotation of the proposed candidate genes to derive features involved in maintaining Ca and P balance. This gene information can be exploited to diagnose and predict characteristics of micronutrient utilization, bone development, and a well-functioning musculoskeletal system in pig husbandry and breeding. |
| first_indexed | 2024-04-13T02:17:17Z |
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| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-13T02:17:17Z |
| publishDate | 2019-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-b5b07886ba0a48e9ad25e4c016d1ca562022-12-22T03:07:07ZengFrontiers Media S.A.Frontiers in Genetics1664-80212019-06-011010.3389/fgene.2019.00590437186Genetic Contribution to Variation in Blood Calcium, Phosphorus, and Alkaline Phosphatase Activity in PigsHenry Reyer0Michael Oster1Dörte Wittenburg2Eduard Murani3Siriluck Ponsuksili4Klaus Wimmers5Klaus Wimmers6Genomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, Dummerstorf, GermanyGenomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, Dummerstorf, GermanyBiomathematics and Bioinformatics Unit, Institute of Genetics and Biometry, Leibniz Institute for Farm Animal Biology, Dummerstorf, GermanyGenomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, Dummerstorf, GermanyFunctional Genome Analysis Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, Dummerstorf, GermanyGenomics Unit, Institute for Genome Biology, Leibniz Institute for Farm Animal Biology, Dummerstorf, GermanyDepartment of Animal Breeding and Genetics, Faculty of Agricultural and Environmental Sciences, University of Rostock, Rostock, GermanyBlood values of calcium (Ca), inorganic phosphorus (IP), and alkaline phosphatase activity (ALP) are valuable indicators for mineral status and bone mineralization. The mineral homeostasis is maintained by absorption, retention, and excretion processes employing a number of known and unknown sensing and regulating factors with implications on immunity. Due to the high inter-individual variation of Ca and P levels in the blood of pigs and to clarify molecular contributions to this variation, the genetics of hematological traits related to the Ca and P balance were investigated in a German Landrace population, integrating both single-locus and multi-locus genome-wide association study (GWAS) approaches. Genomic heritability estimates suggest a moderate genetic contribution to the variation of hematological Ca (N = 456), IP (N = 1049), ALP (N = 439), and the Ca/P ratio (N = 455), with values ranging from 0.27 to 0.54. The genome-wide analysis of markers adds a number of genomic regions to the list of quantitative trait loci, some of which overlap with previous results. Despite the gaps in knowledge of genes involved in Ca and P metabolism, genes like THBS2, SHH, PTPRT, PTGS1, and FRAS1 with reported connections to bone metabolism were derived from the significantly associated genomic regions. Additionally, genomic regions included TRAFD1 and genes coding for phosphate transporters (SLC17A1–SLC17A4), which are linked to Ca and P homeostasis. The study calls for improved functional annotation of the proposed candidate genes to derive features involved in maintaining Ca and P balance. This gene information can be exploited to diagnose and predict characteristics of micronutrient utilization, bone development, and a well-functioning musculoskeletal system in pig husbandry and breeding.https://www.frontiersin.org/article/10.3389/fgene.2019.00590/fullmineralsgeneticspigsgenome-wide associationgenomic heritabilityhematological traits |
| spellingShingle | Henry Reyer Michael Oster Dörte Wittenburg Eduard Murani Siriluck Ponsuksili Klaus Wimmers Klaus Wimmers Genetic Contribution to Variation in Blood Calcium, Phosphorus, and Alkaline Phosphatase Activity in Pigs Frontiers in Genetics minerals genetics pigs genome-wide association genomic heritability hematological traits |
| title | Genetic Contribution to Variation in Blood Calcium, Phosphorus, and Alkaline Phosphatase Activity in Pigs |
| title_full | Genetic Contribution to Variation in Blood Calcium, Phosphorus, and Alkaline Phosphatase Activity in Pigs |
| title_fullStr | Genetic Contribution to Variation in Blood Calcium, Phosphorus, and Alkaline Phosphatase Activity in Pigs |
| title_full_unstemmed | Genetic Contribution to Variation in Blood Calcium, Phosphorus, and Alkaline Phosphatase Activity in Pigs |
| title_short | Genetic Contribution to Variation in Blood Calcium, Phosphorus, and Alkaline Phosphatase Activity in Pigs |
| title_sort | genetic contribution to variation in blood calcium phosphorus and alkaline phosphatase activity in pigs |
| topic | minerals genetics pigs genome-wide association genomic heritability hematological traits |
| url | https://www.frontiersin.org/article/10.3389/fgene.2019.00590/full |
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