Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2)
The purpose of this study is to screen and to evaluate anticancer activity and apoptosis induction of Chromolaena odorata (C. odorata) leaves extract and its fractions on HepG2 cancer cell lines. The C. odorata leaves were extracted by maceration using ethanol 80% then fractionated by using n-hexane...
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| Format: | Article |
| Language: | English |
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Universitas Syiah Kuala, Faculty of Mathematics and Natural Science
2022-03-01
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| Series: | Jurnal Natural |
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| Online Access: | http://jurnal.unsyiah.ac.id/natural/article/view/22854 |
| _version_ | 1827295779690119168 |
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| author | HANIFAH YUSUF MARHAMI FAHRIANI |
| author_facet | HANIFAH YUSUF MARHAMI FAHRIANI |
| author_sort | HANIFAH YUSUF |
| collection | DOAJ |
| description | The purpose of this study is to screen and to evaluate anticancer activity and apoptosis induction of Chromolaena odorata (C. odorata) leaves extract and its fractions on HepG2 cancer cell lines. The C. odorata leaves were extracted by maceration using ethanol 80% then fractionated by using n-hexane, ethyl acetate and ethanol. MTT assay method was used to evaluate anticancer activity and flowcytometri method used to evaluate the induction of apoptosis. The phytochemical analysis and characterization of the major compound of the extract and the fractions were done by using Liquid Chromatography-Mass Spectroscopy (LCMS). The phytochemical analyis showed that the crude ethanolic extract and fractions of C. odorata leaves contains alkaloid, flavonoids, phenolic, steroids, tannin and saponin. The result of LCMS shown the major compounds of the extract and fractions are 5,7,8,3′,4′-Pentamethoxyflavonone, 1-Carboethoxy-β-carboline, 3-Methylcanthin-2, 6- dion, Canthin-6-one. In the n-hexane fraction obtained 5,7,8,3′,4′-Pentamethoxyflavonone, Methyl ophiopogonanone A and Pd-C-Ⅱ. Meanwhile, in the ethyl acetate fraction contained, 3-(3′,4′-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one, Methyl opiopogonanone A and Sinensetin. The ethanol fraction, contained the compound 3-(3′,4′-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one and Methyl opiopogonanone A. The crude ethanolic extract, n-hexane fraction, ethyl acetate and ethanol fraction extract have anticancer activities with IC50 value of 23.44 mg/mL, 84.52mg/mL; 88.51mg/mL; and 167.49 mg/mL respectively. This research suggests the crude ethanolic extract of Chromolaena odorata leaves has a potential candidate for hepatocarcinoma treatment. |
| first_indexed | 2024-04-24T14:27:06Z |
| format | Article |
| id | doaj.art-b5b6fb7a4943440ab8c5f8c0804fada8 |
| institution | Directory Open Access Journal |
| issn | 1411-8513 2541-4062 |
| language | English |
| last_indexed | 2024-04-24T14:27:06Z |
| publishDate | 2022-03-01 |
| publisher | Universitas Syiah Kuala, Faculty of Mathematics and Natural Science |
| record_format | Article |
| series | Jurnal Natural |
| spelling | doaj.art-b5b6fb7a4943440ab8c5f8c0804fada82024-04-03T04:42:14ZengUniversitas Syiah Kuala, Faculty of Mathematics and Natural ScienceJurnal Natural1411-85132541-40622022-03-01221576710.24815/jn.v22i1.2285414004Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2)HANIFAH YUSUF0MARHAMI FAHRIANI1Department of Pharmacology, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, IndonesiaMedical Research Unit, Faculty of Medicine, Universitas Syiah Kuala, Banda Aceh, IndonesiaThe purpose of this study is to screen and to evaluate anticancer activity and apoptosis induction of Chromolaena odorata (C. odorata) leaves extract and its fractions on HepG2 cancer cell lines. The C. odorata leaves were extracted by maceration using ethanol 80% then fractionated by using n-hexane, ethyl acetate and ethanol. MTT assay method was used to evaluate anticancer activity and flowcytometri method used to evaluate the induction of apoptosis. The phytochemical analysis and characterization of the major compound of the extract and the fractions were done by using Liquid Chromatography-Mass Spectroscopy (LCMS). The phytochemical analyis showed that the crude ethanolic extract and fractions of C. odorata leaves contains alkaloid, flavonoids, phenolic, steroids, tannin and saponin. The result of LCMS shown the major compounds of the extract and fractions are 5,7,8,3′,4′-Pentamethoxyflavonone, 1-Carboethoxy-β-carboline, 3-Methylcanthin-2, 6- dion, Canthin-6-one. In the n-hexane fraction obtained 5,7,8,3′,4′-Pentamethoxyflavonone, Methyl ophiopogonanone A and Pd-C-Ⅱ. Meanwhile, in the ethyl acetate fraction contained, 3-(3′,4′-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one, Methyl opiopogonanone A and Sinensetin. The ethanol fraction, contained the compound 3-(3′,4′-Dihydroxybenzyl)-7-hydroxychroman-4-one, 3-(4'-Hydroxy-benzyl)-5,7-dihydroxy-6-methyl-8- methoxy-chroman-4-one and Methyl opiopogonanone A. The crude ethanolic extract, n-hexane fraction, ethyl acetate and ethanol fraction extract have anticancer activities with IC50 value of 23.44 mg/mL, 84.52mg/mL; 88.51mg/mL; and 167.49 mg/mL respectively. This research suggests the crude ethanolic extract of Chromolaena odorata leaves has a potential candidate for hepatocarcinoma treatment.http://jurnal.unsyiah.ac.id/natural/article/view/22854anticancer activity, cancer cell lines, chromolaena odorata linn., hepatocarcinoma |
| spellingShingle | HANIFAH YUSUF MARHAMI FAHRIANI Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2) Jurnal Natural anticancer activity, cancer cell lines, chromolaena odorata linn., hepatocarcinoma |
| title | Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2) |
| title_full | Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2) |
| title_fullStr | Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2) |
| title_full_unstemmed | Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2) |
| title_short | Anticancer activity and apoptotic induction of Chromolaena odorata Linn leaves extract and fractions on hepatocellular carcinoma cell lines (HepG2) |
| title_sort | anticancer activity and apoptotic induction of chromolaena odorata linn leaves extract and fractions on hepatocellular carcinoma cell lines hepg2 |
| topic | anticancer activity, cancer cell lines, chromolaena odorata linn., hepatocarcinoma |
| url | http://jurnal.unsyiah.ac.id/natural/article/view/22854 |
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