Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan.
Madurella mycetomatis is one of the main causative agents of mycetoma, a debilitating neglected tropical disease. Improved understanding of the genomic diversity of the fungal and bacterial causes of mycetoma is essential to advances in diagnosis and treatment. Here, we describe a high-quality genom...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-11-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0010787 |
| _version_ | 1797988512475119616 |
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| author | Anastasia P Litvintseva Sahar Bakhiet Lalitha Gade Darlene D Wagner Ujwal R Bagal Dhwani Batra Emily Norris Lavanya Rishishwar Karlyn D Beer Emmanuel Edwar Siddig Najwa Adam Mhmoud Nancy A Chow Ahmed Fahal |
| author_facet | Anastasia P Litvintseva Sahar Bakhiet Lalitha Gade Darlene D Wagner Ujwal R Bagal Dhwani Batra Emily Norris Lavanya Rishishwar Karlyn D Beer Emmanuel Edwar Siddig Najwa Adam Mhmoud Nancy A Chow Ahmed Fahal |
| author_sort | Anastasia P Litvintseva |
| collection | DOAJ |
| description | Madurella mycetomatis is one of the main causative agents of mycetoma, a debilitating neglected tropical disease. Improved understanding of the genomic diversity of the fungal and bacterial causes of mycetoma is essential to advances in diagnosis and treatment. Here, we describe a high-quality genome assembly of M. mycetomatis and results of the whole genome sequence analysis of 26 isolates from Sudan. We demonstrate evidence of at least seven genetically diverse lineages and extreme clonality among isolates within these lineages. We also performed shotgun metagenomic analysis of DNA extracted from mycetoma grains and showed that M. mycetomatis reads were detected in all sequenced samples with the average of 11,317 reads (s.d. +/- 21,269) per sample. In addition, 10 (12%) of the 81 tested grain samples contained bacterial reads including Streptococcus sp., Staphylococcus sp. and others. |
| first_indexed | 2024-04-11T08:05:04Z |
| format | Article |
| id | doaj.art-b5ba4c8fced94547831c827992b73506 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-11T08:05:04Z |
| publishDate | 2022-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-b5ba4c8fced94547831c827992b735062022-12-22T04:35:36ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352022-11-011611e001078710.1371/journal.pntd.0010787Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan.Anastasia P LitvintsevaSahar BakhietLalitha GadeDarlene D WagnerUjwal R BagalDhwani BatraEmily NorrisLavanya RishishwarKarlyn D BeerEmmanuel Edwar SiddigNajwa Adam MhmoudNancy A ChowAhmed FahalMadurella mycetomatis is one of the main causative agents of mycetoma, a debilitating neglected tropical disease. Improved understanding of the genomic diversity of the fungal and bacterial causes of mycetoma is essential to advances in diagnosis and treatment. Here, we describe a high-quality genome assembly of M. mycetomatis and results of the whole genome sequence analysis of 26 isolates from Sudan. We demonstrate evidence of at least seven genetically diverse lineages and extreme clonality among isolates within these lineages. We also performed shotgun metagenomic analysis of DNA extracted from mycetoma grains and showed that M. mycetomatis reads were detected in all sequenced samples with the average of 11,317 reads (s.d. +/- 21,269) per sample. In addition, 10 (12%) of the 81 tested grain samples contained bacterial reads including Streptococcus sp., Staphylococcus sp. and others.https://doi.org/10.1371/journal.pntd.0010787 |
| spellingShingle | Anastasia P Litvintseva Sahar Bakhiet Lalitha Gade Darlene D Wagner Ujwal R Bagal Dhwani Batra Emily Norris Lavanya Rishishwar Karlyn D Beer Emmanuel Edwar Siddig Najwa Adam Mhmoud Nancy A Chow Ahmed Fahal Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan. PLoS Neglected Tropical Diseases |
| title | Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan. |
| title_full | Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan. |
| title_fullStr | Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan. |
| title_full_unstemmed | Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan. |
| title_short | Genomics and metagenomics of Madurella mycetomatis, a causative agent of black grain mycetoma in Sudan. |
| title_sort | genomics and metagenomics of madurella mycetomatis a causative agent of black grain mycetoma in sudan |
| url | https://doi.org/10.1371/journal.pntd.0010787 |
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