IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis
The aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants. The children were submitted to clinical examinations to assess macular, neurological and hearing signals. The WB results obtained were compared to...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2010-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600005 |
| _version_ | 1797713506602057728 |
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| author | Anderson S Machado Gláucia MQ Andrade José N Januário Matheus D Fernandes Ana Carolina AV Carneiro Mariângela Carneiro Ericka VM Carellos Roberta MC Romanelli Daniel V Vasconcelos-Santos Ricardo WA Vitor |
| author_facet | Anderson S Machado Gláucia MQ Andrade José N Januário Matheus D Fernandes Ana Carolina AV Carneiro Mariângela Carneiro Ericka VM Carellos Roberta MC Romanelli Daniel V Vasconcelos-Santos Ricardo WA Vitor |
| author_sort | Anderson S Machado |
| collection | DOAJ |
| description | The aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants. The children were submitted to clinical examinations to assess macular, neurological and hearing signals. The WB results obtained were compared to the persistence of IgG antibodies at the end of 12 months, which is regarded as the "gold standard" diagnosis of congenital toxoplasmosis. Association between the WB results and the clinical signs presented by the infants was also assessed. Of the 215 children, 177 had a confirmed congenital toxoplasmosis diagnosis and 38 were uninfected. IgG-WB showed a sensitivity of 73.5% and a specificity of 97.4%. IgM-WB showed a sensitivity of 54.8% and a specificity of 94.7%. The IgG-WB and IgM-WB combination increased the sensitivity to 86.5%. The IgM-WB-positive children had a 1.4-fold greater risk of presenting active macular lesions than did those that were IgM-WB-negative. This study showed that the WB assay is a useful tool to confirm a diagnosis of congenital toxoplasmosis and that the IgM-WB-positive results can indicate active macular lesions in newborn infants. |
| first_indexed | 2024-03-12T07:37:37Z |
| format | Article |
| id | doaj.art-b5bb1ef6ee494e0e9dbd39fb281ccb8d |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T07:37:37Z |
| publishDate | 2010-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-b5bb1ef6ee494e0e9dbd39fb281ccb8d2023-09-02T21:28:09ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602010-09-01105675776110.1590/S0074-02762010000600005IgG and IgM western blot assay for diagnosis of congenital toxoplasmosisAnderson S MachadoGláucia MQ AndradeJosé N JanuárioMatheus D FernandesAna Carolina AV CarneiroMariângela CarneiroEricka VM CarellosRoberta MC RomanelliDaniel V Vasconcelos-SantosRicardo WA VitorThe aim of this study was to evaluate the utility of western blot (WB) analysis as a diagnostic tool for congenital toxoplasmosis in 215 newborn infants. The children were submitted to clinical examinations to assess macular, neurological and hearing signals. The WB results obtained were compared to the persistence of IgG antibodies at the end of 12 months, which is regarded as the "gold standard" diagnosis of congenital toxoplasmosis. Association between the WB results and the clinical signs presented by the infants was also assessed. Of the 215 children, 177 had a confirmed congenital toxoplasmosis diagnosis and 38 were uninfected. IgG-WB showed a sensitivity of 73.5% and a specificity of 97.4%. IgM-WB showed a sensitivity of 54.8% and a specificity of 94.7%. The IgG-WB and IgM-WB combination increased the sensitivity to 86.5%. The IgM-WB-positive children had a 1.4-fold greater risk of presenting active macular lesions than did those that were IgM-WB-negative. This study showed that the WB assay is a useful tool to confirm a diagnosis of congenital toxoplasmosis and that the IgM-WB-positive results can indicate active macular lesions in newborn infants.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600005congenital toxoplasmosiswestern blotmacular toxoplasmosis |
| spellingShingle | Anderson S Machado Gláucia MQ Andrade José N Januário Matheus D Fernandes Ana Carolina AV Carneiro Mariângela Carneiro Ericka VM Carellos Roberta MC Romanelli Daniel V Vasconcelos-Santos Ricardo WA Vitor IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis Memorias do Instituto Oswaldo Cruz congenital toxoplasmosis western blot macular toxoplasmosis |
| title | IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis |
| title_full | IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis |
| title_fullStr | IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis |
| title_full_unstemmed | IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis |
| title_short | IgG and IgM western blot assay for diagnosis of congenital toxoplasmosis |
| title_sort | igg and igm western blot assay for diagnosis of congenital toxoplasmosis |
| topic | congenital toxoplasmosis western blot macular toxoplasmosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762010000600005 |
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