Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategy
Escopoletin, a phenolic compound belonging to anthocyanin family shows promising antioxidant activities. In the present study, anti-cancer effects of escopoletin treatment in DU145 cells were investigated. The sulphorhodamine-B staining and annexin V and propidium iodide were respectively used for t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2015-07-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/23095 |
| _version_ | 1811317646523629568 |
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| author | Da Chen Xiao-Yi Zhang Fa-Zhu Zheng Hai-Tao Wang Jian-Liang Cai Ming Xia |
| author_facet | Da Chen Xiao-Yi Zhang Fa-Zhu Zheng Hai-Tao Wang Jian-Liang Cai Ming Xia |
| author_sort | Da Chen |
| collection | DOAJ |
| description | Escopoletin, a phenolic compound belonging to anthocyanin family shows promising antioxidant activities. In the present study, anti-cancer effects of escopoletin treatment in DU145 cells were investigated. The sulphorhodamine-B staining and annexin V and propidium iodide were respectively used for the analysis of cell viability and death. The results revealed a significantly higher cytotoxicity by escopoletin that caused cell death in DU145 cells. Escopoletin treatment in DU145 cells markedly inhibited cell growth through non-apoptotic cell death and induced significant reactive oxygen species (ROS) production. It also induced G1 cell cycle arrest and cyclin D1 accumulation through the enhanced expression of p21. However, the effect of escopoletin on DU145 cells was reversed by pretreatment with glutathione antioxidant. This suggests that escopoletin induced generation of ROS is responsible for the increased cytotoxicity in DU145 cells. Thus, escopoletin exhibits potential therapeutic efficacy for the treatment of prostate cancer. |
| first_indexed | 2024-04-13T12:11:53Z |
| format | Article |
| id | doaj.art-b5bb7a6e140b48f1ae73e4a1f0f2b951 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-04-13T12:11:53Z |
| publishDate | 2015-07-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-b5bb7a6e140b48f1ae73e4a1f0f2b9512022-12-22T02:47:27ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882015-07-01103Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategyDa Chen0Xiao-Yi Zhang1Fa-Zhu Zheng2Hai-Tao Wang3Jian-Liang Cai4Ming Xia5Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038Department of Urology, The Second Artillery General Hospital PLA, Beijing 100088Department of Urology Handan Peoples? Hospital, Handan 056001Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038Department of Urology, Beijing Shijitan Hospital, Capital Medical University. Beijing 100038Escopoletin, a phenolic compound belonging to anthocyanin family shows promising antioxidant activities. In the present study, anti-cancer effects of escopoletin treatment in DU145 cells were investigated. The sulphorhodamine-B staining and annexin V and propidium iodide were respectively used for the analysis of cell viability and death. The results revealed a significantly higher cytotoxicity by escopoletin that caused cell death in DU145 cells. Escopoletin treatment in DU145 cells markedly inhibited cell growth through non-apoptotic cell death and induced significant reactive oxygen species (ROS) production. It also induced G1 cell cycle arrest and cyclin D1 accumulation through the enhanced expression of p21. However, the effect of escopoletin on DU145 cells was reversed by pretreatment with glutathione antioxidant. This suggests that escopoletin induced generation of ROS is responsible for the increased cytotoxicity in DU145 cells. Thus, escopoletin exhibits potential therapeutic efficacy for the treatment of prostate cancer.https://www.banglajol.info/index.php/BJP/article/view/23095AntioxidantCancerCell viabilityEscopoletinProstate |
| spellingShingle | Da Chen Xiao-Yi Zhang Fa-Zhu Zheng Hai-Tao Wang Jian-Liang Cai Ming Xia Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategy Bangladesh Journal of Pharmacology Antioxidant Cancer Cell viability Escopoletin Prostate |
| title | Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategy |
| title_full | Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategy |
| title_fullStr | Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategy |
| title_full_unstemmed | Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategy |
| title_short | Induction of cell death in prostate cancer cells by escopoletin, a promising treatment strategy |
| title_sort | induction of cell death in prostate cancer cells by escopoletin a promising treatment strategy |
| topic | Antioxidant Cancer Cell viability Escopoletin Prostate |
| url | https://www.banglajol.info/index.php/BJP/article/view/23095 |
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