Rapid identification of Psathyrostachys huashanica Keng chromosomes in wheat background based on ND-FISH and SNP array methods
Psathyrostachys huashanica Keng (2n=2x=14, NsNs) is regarded as a valuable wild relative species for common wheat cultivar improvement because of its abundant beneficial agronomic traits. However, although the development of many wheat–P. huashanica-derived lines provides a germplasm base for the tr...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311923000138 |
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| author | Jia-chuang LI Jiao-jiao LI Li ZHAO Ji-xin ZHAO Jun WU Xin-hong CHEN Li-yu ZHANG Pu-hui DONG Li-ming WANG De-hui ZHAO Chun-ping WANG Yu-hui PANG |
| author_facet | Jia-chuang LI Jiao-jiao LI Li ZHAO Ji-xin ZHAO Jun WU Xin-hong CHEN Li-yu ZHANG Pu-hui DONG Li-ming WANG De-hui ZHAO Chun-ping WANG Yu-hui PANG |
| author_sort | Jia-chuang LI |
| collection | DOAJ |
| description | Psathyrostachys huashanica Keng (2n=2x=14, NsNs) is regarded as a valuable wild relative species for common wheat cultivar improvement because of its abundant beneficial agronomic traits. However, although the development of many wheat–P. huashanica-derived lines provides a germplasm base for the transfer of excellent traits, the lag in the identification of P. huashanica chromosomes in the wheat background has limited the study of these lines. In this study, three novel nondenaturing fluorescence in situ hybridization (ND-FISH)-positive oligo probes were developed. Among them, HS-TZ3 and HS-TZ4 could specifically hybridize with P. huashanica chromosomes, mainly in the telomere area, and HS-CHTZ5 could hybridize with the chromosomal centromere area. We sequentially constructed a P. huashanica FISH karyotype and idiogram that helped identify the homologous groups of introduced P. huashanica chromosomes. In detail, 1Ns and 2Ns had opposite signals on the short and long arms, 3Ns, 4Ns, and 7Ns had superposed two-color signals, 5Ns and 6Ns had fluorescent signals only on their short arms, and 7Ns had signals on the intercalary of the long arm. In addition, we evaluated different ways to identify alien introgression lines by using low-density single nucleotide polymorphism (SNP) arrays and recommended the SNP homozygosity rate in each chromosome as a statistical pattern. The 15K SNP array is widely applicable for addition, substitution, and translocation lines, and the 40K SNP array is the most accurate for recognizing transposed intervals between wheat and alien chromosomes. Our research provided convenient methods to distinguish the homologous group of P. huashanica chromosomes in a common wheat background based on ND-FISH and SNP arrays, which is of great significance for efficiently identifying wheat–P. huashanica-derived lines and the further application of Ns chromosomes. |
| first_indexed | 2024-03-11T18:29:13Z |
| format | Article |
| id | doaj.art-b5bbd6d960e643e7aa0a0e624decd05f |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-03-11T18:29:13Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-b5bbd6d960e643e7aa0a0e624decd05f2023-10-13T13:53:11ZengElsevierJournal of Integrative Agriculture2095-31192023-10-01221029342948Rapid identification of Psathyrostachys huashanica Keng chromosomes in wheat background based on ND-FISH and SNP array methodsJia-chuang LI0Jiao-jiao LI1Li ZHAO2Ji-xin ZHAO3Jun WU4Xin-hong CHEN5Li-yu ZHANG6Pu-hui DONG7Li-ming WANG8De-hui ZHAO9Chun-ping WANG10Yu-hui PANG11College of Agronomy, Henan University of Science and Technology, Luoyang 471023, P.R.China; The Shennong Laboratory, Henan Provience, Zhengzhou 450002, P.R.ChinaCollege of Agronomy, Henan University of Science and Technology, Luoyang 471023, P.R.ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Agronomy, Northwest A&F University, Yangling 712100, P.R.ChinaCollege of Agronomy, Henan University of Science and Technology, Luoyang 471023, P.R.ChinaCollege of Agronomy, Henan University of Science and Technology, Luoyang 471023, P.R.ChinaCollege of Agronomy, Henan University of Science and Technology, Luoyang 471023, P.R.ChinaCollege of Agronomy, Henan University of Science and Technology, Luoyang 471023, P.R.China; The Shennong Laboratory, Henan Provience, Zhengzhou 450002, P.R.ChinaCollege of Agronomy, Henan University of Science and Technology, Luoyang 471023, P.R.China; The Shennong Laboratory, Henan Provience, Zhengzhou 450002, P.R.China; Correspondence WANG Chun-pingCollege of Agronomy, Henan University of Science and Technology, Luoyang 471023, P.R.China; The Shennong Laboratory, Henan Provience, Zhengzhou 450002, P.R.China; Correspondence PANG Yu-huiPsathyrostachys huashanica Keng (2n=2x=14, NsNs) is regarded as a valuable wild relative species for common wheat cultivar improvement because of its abundant beneficial agronomic traits. However, although the development of many wheat–P. huashanica-derived lines provides a germplasm base for the transfer of excellent traits, the lag in the identification of P. huashanica chromosomes in the wheat background has limited the study of these lines. In this study, three novel nondenaturing fluorescence in situ hybridization (ND-FISH)-positive oligo probes were developed. Among them, HS-TZ3 and HS-TZ4 could specifically hybridize with P. huashanica chromosomes, mainly in the telomere area, and HS-CHTZ5 could hybridize with the chromosomal centromere area. We sequentially constructed a P. huashanica FISH karyotype and idiogram that helped identify the homologous groups of introduced P. huashanica chromosomes. In detail, 1Ns and 2Ns had opposite signals on the short and long arms, 3Ns, 4Ns, and 7Ns had superposed two-color signals, 5Ns and 6Ns had fluorescent signals only on their short arms, and 7Ns had signals on the intercalary of the long arm. In addition, we evaluated different ways to identify alien introgression lines by using low-density single nucleotide polymorphism (SNP) arrays and recommended the SNP homozygosity rate in each chromosome as a statistical pattern. The 15K SNP array is widely applicable for addition, substitution, and translocation lines, and the 40K SNP array is the most accurate for recognizing transposed intervals between wheat and alien chromosomes. Our research provided convenient methods to distinguish the homologous group of P. huashanica chromosomes in a common wheat background based on ND-FISH and SNP arrays, which is of great significance for efficiently identifying wheat–P. huashanica-derived lines and the further application of Ns chromosomes.http://www.sciencedirect.com/science/article/pii/S2095311923000138Psathyrostachys huashanicaNs chromosomesND-FISHSNP arraycommon wheat |
| spellingShingle | Jia-chuang LI Jiao-jiao LI Li ZHAO Ji-xin ZHAO Jun WU Xin-hong CHEN Li-yu ZHANG Pu-hui DONG Li-ming WANG De-hui ZHAO Chun-ping WANG Yu-hui PANG Rapid identification of Psathyrostachys huashanica Keng chromosomes in wheat background based on ND-FISH and SNP array methods Journal of Integrative Agriculture Psathyrostachys huashanica Ns chromosomes ND-FISH SNP array common wheat |
| title | Rapid identification of Psathyrostachys huashanica Keng chromosomes in wheat background based on ND-FISH and SNP array methods |
| title_full | Rapid identification of Psathyrostachys huashanica Keng chromosomes in wheat background based on ND-FISH and SNP array methods |
| title_fullStr | Rapid identification of Psathyrostachys huashanica Keng chromosomes in wheat background based on ND-FISH and SNP array methods |
| title_full_unstemmed | Rapid identification of Psathyrostachys huashanica Keng chromosomes in wheat background based on ND-FISH and SNP array methods |
| title_short | Rapid identification of Psathyrostachys huashanica Keng chromosomes in wheat background based on ND-FISH and SNP array methods |
| title_sort | rapid identification of psathyrostachys huashanica keng chromosomes in wheat background based on nd fish and snp array methods |
| topic | Psathyrostachys huashanica Ns chromosomes ND-FISH SNP array common wheat |
| url | http://www.sciencedirect.com/science/article/pii/S2095311923000138 |
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