Probiotic <i>Bacillus</i> Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model
Emerging evidence suggests a key role of gut microbiota in maintaining liver functions through modulating the gut–liver axis. In this study, we investigated whether microbiota alteration mediated by probiotic <i>Bacillus</i> was involved in alleviating oxidative stress- induced liver inj...
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MDPI AG
2022-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/2/291 |
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| author | Yanping Wu Baikui Wang Li Tang Yuanhao Zhou Qi Wang Li Gong Jiajia Ni Weifen Li |
| author_facet | Yanping Wu Baikui Wang Li Tang Yuanhao Zhou Qi Wang Li Gong Jiajia Ni Weifen Li |
| author_sort | Yanping Wu |
| collection | DOAJ |
| description | Emerging evidence suggests a key role of gut microbiota in maintaining liver functions through modulating the gut–liver axis. In this study, we investigated whether microbiota alteration mediated by probiotic <i>Bacillus</i> was involved in alleviating oxidative stress- induced liver injury. Sprague–Dawley rats were orally administered <i>Bacillus</i> SC06 or SC08 for a 24-day period and thereafter intraperitoneally injected diquat (DQ) to induce oxidative stress. Results showed that <i>Bacillus</i>, particularly SC06 significantly inhibited hepatic injuries, as evidenced by the alleviated damaged liver structure, the decreased levels of ALT, AST, ALP and LDH, and the suppressed mitochondrial dysfunction. SC06 pretreatment markedly enhanced the liver antioxidant capacity by decreasing MDA and p47, and increasing T-AOC, SOD and HO-1.16S rRNA sequencing analysis revealed that DQ significantly changed the diversities and composition of gut microbiota, whereas <i>Bacillus</i> pretreatments could attenuate gut dysbiosis. Pearson’s correlation analysis showed that AST and MDA exerted a positive correlation with the opportunistic pathogenic genera and species (<i>Escherichia</i> and <i>Shigella</i>), and negatively correlated with the potential probiotics (<i>Lactobacillus</i>), while SOD exerted a reverse trend. The microbial metagenomic analysis demonstrated that <i>Bacillus</i>, particularly SC06 markedly suppress the metabolic pathways such as carbohydrate metabolism, lipid metabolism, amino acid metabolism and metabolism of cofactors and vitamins. Furthermore, SC06 decreased the gene abundance of the pathways mediating bacterial replication, secretion and pathogenicity. Taken together, <i>Bacillus</i> SC06 alleviates oxidative stress-induced liver injuries via optimizing the composition, metabolic pathways and pathogenic replication and secretion of gut microbiota. These findings elucidate the mechanisms of probiotics in alleviating oxidative stress and provide a promising strategy for preventing liver diseases by targeting gut microbiota. |
| first_indexed | 2024-03-09T22:44:49Z |
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| last_indexed | 2024-03-09T22:44:49Z |
| publishDate | 2022-01-01 |
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| series | Antioxidants |
| spelling | doaj.art-b5bd7181770d44a0943e0d3a1061228f2023-11-23T18:31:26ZengMDPI AGAntioxidants2076-39212022-01-0111229110.3390/antiox11020291Probiotic <i>Bacillus</i> Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat ModelYanping Wu0Baikui Wang1Li Tang2Yuanhao Zhou3Qi Wang4Li Gong5Jiajia Ni6Weifen Li7Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaResearch and Development Center, Guangdong Meilikang Bio-Science Ltd., Dongguan 523808, ChinaKey Laboratory of Molecular Animal Nutrition of the Ministry of Education, College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaEmerging evidence suggests a key role of gut microbiota in maintaining liver functions through modulating the gut–liver axis. In this study, we investigated whether microbiota alteration mediated by probiotic <i>Bacillus</i> was involved in alleviating oxidative stress- induced liver injury. Sprague–Dawley rats were orally administered <i>Bacillus</i> SC06 or SC08 for a 24-day period and thereafter intraperitoneally injected diquat (DQ) to induce oxidative stress. Results showed that <i>Bacillus</i>, particularly SC06 significantly inhibited hepatic injuries, as evidenced by the alleviated damaged liver structure, the decreased levels of ALT, AST, ALP and LDH, and the suppressed mitochondrial dysfunction. SC06 pretreatment markedly enhanced the liver antioxidant capacity by decreasing MDA and p47, and increasing T-AOC, SOD and HO-1.16S rRNA sequencing analysis revealed that DQ significantly changed the diversities and composition of gut microbiota, whereas <i>Bacillus</i> pretreatments could attenuate gut dysbiosis. Pearson’s correlation analysis showed that AST and MDA exerted a positive correlation with the opportunistic pathogenic genera and species (<i>Escherichia</i> and <i>Shigella</i>), and negatively correlated with the potential probiotics (<i>Lactobacillus</i>), while SOD exerted a reverse trend. The microbial metagenomic analysis demonstrated that <i>Bacillus</i>, particularly SC06 markedly suppress the metabolic pathways such as carbohydrate metabolism, lipid metabolism, amino acid metabolism and metabolism of cofactors and vitamins. Furthermore, SC06 decreased the gene abundance of the pathways mediating bacterial replication, secretion and pathogenicity. Taken together, <i>Bacillus</i> SC06 alleviates oxidative stress-induced liver injuries via optimizing the composition, metabolic pathways and pathogenic replication and secretion of gut microbiota. These findings elucidate the mechanisms of probiotics in alleviating oxidative stress and provide a promising strategy for preventing liver diseases by targeting gut microbiota.https://www.mdpi.com/2076-3921/11/2/291<i>Bacillus</i>ratsoxidative stressliver injurygut microbiota |
| spellingShingle | Yanping Wu Baikui Wang Li Tang Yuanhao Zhou Qi Wang Li Gong Jiajia Ni Weifen Li Probiotic <i>Bacillus</i> Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model Antioxidants <i>Bacillus</i> rats oxidative stress liver injury gut microbiota |
| title | Probiotic <i>Bacillus</i> Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model |
| title_full | Probiotic <i>Bacillus</i> Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model |
| title_fullStr | Probiotic <i>Bacillus</i> Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model |
| title_full_unstemmed | Probiotic <i>Bacillus</i> Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model |
| title_short | Probiotic <i>Bacillus</i> Alleviates Oxidative Stress-Induced Liver Injury by Modulating Gut-Liver Axis in a Rat Model |
| title_sort | probiotic i bacillus i alleviates oxidative stress induced liver injury by modulating gut liver axis in a rat model |
| topic | <i>Bacillus</i> rats oxidative stress liver injury gut microbiota |
| url | https://www.mdpi.com/2076-3921/11/2/291 |
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