The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway
Abstract Background Acute lung injury is an important factor that leads to the death of patients with pneumonia. Previous studies have shown that nicotinamide (NAM) plays a role in reducing cell damage, so this study explored the mechanism by which NAM functions in acute lung injury. Methods We expl...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2021-09-01
|
| Series: | Molecular Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s10020-021-00376-2 |
| _version_ | 1818882175869648896 |
|---|---|
| author | Qun Zhang Junyao Li Haixia Zhong Yanling Xu |
| author_facet | Qun Zhang Junyao Li Haixia Zhong Yanling Xu |
| author_sort | Qun Zhang |
| collection | DOAJ |
| description | Abstract Background Acute lung injury is an important factor that leads to the death of patients with pneumonia. Previous studies have shown that nicotinamide (NAM) plays a role in reducing cell damage, so this study explored the mechanism by which NAM functions in acute lung injury. Methods We explored the mechanism by which NAM affects acute lung injury in vivo and in vitro by qRT-PCR, western blotting and ELISA. Results The results showed that NAM could significantly reduce lung injury and proinflammatory mediator accumulation. Further mechanistic studies showed that NAM could significantly inhibit the MAPK and AKT/NF-κB signaling pathways. Conclusion These results suggested that NAM may reduce the release of proinflammatory mediators by inhibiting the MAPK and AKT/NF-κB signaling pathways and ultimately alleviate lung injury. |
| first_indexed | 2024-12-19T15:13:35Z |
| format | Article |
| id | doaj.art-b5be22cc82fb4b85b5097743167dc745 |
| institution | Directory Open Access Journal |
| issn | 1076-1551 1528-3658 |
| language | English |
| last_indexed | 2024-12-19T15:13:35Z |
| publishDate | 2021-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Medicine |
| spelling | doaj.art-b5be22cc82fb4b85b5097743167dc7452022-12-21T20:16:13ZengBMCMolecular Medicine1076-15511528-36582021-09-0127111110.1186/s10020-021-00376-2The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathwayQun Zhang0Junyao Li1Haixia Zhong2Yanling Xu3Department of Respiratory and Critical Care Medicine, The Second Hospital of Jilin UniversityDepartment of Respiratory and Critical Care Medicine, The Second Hospital of Jilin UniversityDepartment of Respiration, General Hospital of Jilin Tonghua Mining Co., LtdDepartment of Respiratory and Critical Care Medicine, The Second Hospital of Jilin UniversityAbstract Background Acute lung injury is an important factor that leads to the death of patients with pneumonia. Previous studies have shown that nicotinamide (NAM) plays a role in reducing cell damage, so this study explored the mechanism by which NAM functions in acute lung injury. Methods We explored the mechanism by which NAM affects acute lung injury in vivo and in vitro by qRT-PCR, western blotting and ELISA. Results The results showed that NAM could significantly reduce lung injury and proinflammatory mediator accumulation. Further mechanistic studies showed that NAM could significantly inhibit the MAPK and AKT/NF-κB signaling pathways. Conclusion These results suggested that NAM may reduce the release of proinflammatory mediators by inhibiting the MAPK and AKT/NF-κB signaling pathways and ultimately alleviate lung injury.https://doi.org/10.1186/s10020-021-00376-2Acute lung injuryNicotinamideMAPKAKT/NF-κB |
| spellingShingle | Qun Zhang Junyao Li Haixia Zhong Yanling Xu The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway Molecular Medicine Acute lung injury Nicotinamide MAPK AKT/NF-κB |
| title | The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway |
| title_full | The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway |
| title_fullStr | The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway |
| title_full_unstemmed | The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway |
| title_short | The mechanism of nicotinamide on reducing acute lung injury by inhibiting MAPK and NF-κB signal pathway |
| title_sort | mechanism of nicotinamide on reducing acute lung injury by inhibiting mapk and nf κb signal pathway |
| topic | Acute lung injury Nicotinamide MAPK AKT/NF-κB |
| url | https://doi.org/10.1186/s10020-021-00376-2 |
| work_keys_str_mv | AT qunzhang themechanismofnicotinamideonreducingacutelunginjurybyinhibitingmapkandnfkbsignalpathway AT junyaoli themechanismofnicotinamideonreducingacutelunginjurybyinhibitingmapkandnfkbsignalpathway AT haixiazhong themechanismofnicotinamideonreducingacutelunginjurybyinhibitingmapkandnfkbsignalpathway AT yanlingxu themechanismofnicotinamideonreducingacutelunginjurybyinhibitingmapkandnfkbsignalpathway AT qunzhang mechanismofnicotinamideonreducingacutelunginjurybyinhibitingmapkandnfkbsignalpathway AT junyaoli mechanismofnicotinamideonreducingacutelunginjurybyinhibitingmapkandnfkbsignalpathway AT haixiazhong mechanismofnicotinamideonreducingacutelunginjurybyinhibitingmapkandnfkbsignalpathway AT yanlingxu mechanismofnicotinamideonreducingacutelunginjurybyinhibitingmapkandnfkbsignalpathway |