Kuei-Lu-Er-Xian-Jiao extract enhances BMP-2 production in osteoblasts
Osteoporosis is a common skeletal disorder, resulting from an imbalance in bone resorption relative to formation. Bone morphogenetic protein (BMP) is a key regulator in bone formation and osteoblastic differentiation. Hence, compounds that promote BMP expression may be suitable candidates for osteop...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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China Medical University
2017-03-01
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| Series: | BioMedicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1051/bmdcn/2017070102 |
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| author | Wu Min-Huan Lee Ting-Hsuan Lee Hsiang-Ping Li Te-Mao Lee I-Tee Shieh Po-Chuen Tang Chih-Hsin |
| author_facet | Wu Min-Huan Lee Ting-Hsuan Lee Hsiang-Ping Li Te-Mao Lee I-Tee Shieh Po-Chuen Tang Chih-Hsin |
| author_sort | Wu Min-Huan |
| collection | DOAJ |
| description | Osteoporosis is a common skeletal disorder, resulting from an imbalance in bone resorption relative to formation. Bone morphogenetic protein (BMP) is a key regulator in bone formation and osteoblastic differentiation. Hence, compounds that promote BMP expression may be suitable candidates for osteoporosis treatment. This study examined the effects of the traditional Chinese medicinal agent, Kuei-Lu-Er-Xian-Jiao (KLEXJ), on BMP-2 production in osteoblasts. We found that KLEXJ extract promoted osteoblastic differentiation marker ALP activity and increased BMP-2 production; pretreatment with PI3K and Akt inhibitors, or small interfering RNA (siRNA), reduced these effects. KLEXJ also enhanced PI3K and Akt phosphorylation. Treatment of osteoblastic cells with NF-κB inhibitors (TPCK or PDTC) markedly inhibited KLEXJ-enhancement of ALP activity and BMP-2 production. KLEXJ also significantly promoted p65 phosphorylation, while treatment with PI3K and Akt inhibitors antagonized KLEXJ-enhanced p65 phosphorylation. Thus, KLEXJ enhances ALP activity and BMP-2 production of osteoblasts through the PI3K/Akt/ NF-κB signaling pathway and hence may be suitable in the treatment of osteoporosis. |
| first_indexed | 2024-12-21T22:41:29Z |
| format | Article |
| id | doaj.art-b5c21e4ab75744a6bcb105d7d49b400b |
| institution | Directory Open Access Journal |
| issn | 2211-8039 |
| language | English |
| last_indexed | 2024-12-21T22:41:29Z |
| publishDate | 2017-03-01 |
| publisher | China Medical University |
| record_format | Article |
| series | BioMedicine |
| spelling | doaj.art-b5c21e4ab75744a6bcb105d7d49b400b2022-12-21T18:47:49ZengChina Medical UniversityBioMedicine2211-80392017-03-0171210.1051/bmdcn/2017070102bmdcn2017070102Kuei-Lu-Er-Xian-Jiao extract enhances BMP-2 production in osteoblastsWu Min-HuanLee Ting-HsuanLee Hsiang-PingLi Te-MaoLee I-TeeShieh Po-ChuenTang Chih-HsinOsteoporosis is a common skeletal disorder, resulting from an imbalance in bone resorption relative to formation. Bone morphogenetic protein (BMP) is a key regulator in bone formation and osteoblastic differentiation. Hence, compounds that promote BMP expression may be suitable candidates for osteoporosis treatment. This study examined the effects of the traditional Chinese medicinal agent, Kuei-Lu-Er-Xian-Jiao (KLEXJ), on BMP-2 production in osteoblasts. We found that KLEXJ extract promoted osteoblastic differentiation marker ALP activity and increased BMP-2 production; pretreatment with PI3K and Akt inhibitors, or small interfering RNA (siRNA), reduced these effects. KLEXJ also enhanced PI3K and Akt phosphorylation. Treatment of osteoblastic cells with NF-κB inhibitors (TPCK or PDTC) markedly inhibited KLEXJ-enhancement of ALP activity and BMP-2 production. KLEXJ also significantly promoted p65 phosphorylation, while treatment with PI3K and Akt inhibitors antagonized KLEXJ-enhanced p65 phosphorylation. Thus, KLEXJ enhances ALP activity and BMP-2 production of osteoblasts through the PI3K/Akt/ NF-κB signaling pathway and hence may be suitable in the treatment of osteoporosis.https://doi.org/10.1051/bmdcn/2017070102Kuei-Lu-Er-Xian-JiaoOsteoblastsBMP-2ALP activity China Medical University |
| spellingShingle | Wu Min-Huan Lee Ting-Hsuan Lee Hsiang-Ping Li Te-Mao Lee I-Tee Shieh Po-Chuen Tang Chih-Hsin Kuei-Lu-Er-Xian-Jiao extract enhances BMP-2 production in osteoblasts BioMedicine Kuei-Lu-Er-Xian-Jiao Osteoblasts BMP-2 ALP activity China Medical University |
| title | Kuei-Lu-Er-Xian-Jiao extract enhances BMP-2 production in osteoblasts |
| title_full | Kuei-Lu-Er-Xian-Jiao extract enhances BMP-2 production in osteoblasts |
| title_fullStr | Kuei-Lu-Er-Xian-Jiao extract enhances BMP-2 production in osteoblasts |
| title_full_unstemmed | Kuei-Lu-Er-Xian-Jiao extract enhances BMP-2 production in osteoblasts |
| title_short | Kuei-Lu-Er-Xian-Jiao extract enhances BMP-2 production in osteoblasts |
| title_sort | kuei lu er xian jiao extract enhances bmp 2 production in osteoblasts |
| topic | Kuei-Lu-Er-Xian-Jiao Osteoblasts BMP-2 ALP activity China Medical University |
| url | https://doi.org/10.1051/bmdcn/2017070102 |
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