Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition
PAPP-A substrate selectivity underlies the tight regulation of IGF signaling. Here, the authors report cryo-EM structures of dimeric PAPP-A in its substrate-free form and in complex with a peptide substrate, which combined with biochemical assays provide a mechanism for PAPP-A substrate binding and...
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-09-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-33175-2 |
| _version_ | 1828374197262352384 |
|---|---|
| author | Russell A. Judge Janani Sridar Kathryn Tunyasunvunakool Rinku Jain John C. K. Wang Christna Ouch Jun Xu Amirhossein Mafi Aaron H. Nile Clint Remarcik Corey L. Smith Crystal Ghosh Chen Xu Vincent Stoll John Jumper Amoolya H. Singh Dan Eaton Qi Hao |
| author_facet | Russell A. Judge Janani Sridar Kathryn Tunyasunvunakool Rinku Jain John C. K. Wang Christna Ouch Jun Xu Amirhossein Mafi Aaron H. Nile Clint Remarcik Corey L. Smith Crystal Ghosh Chen Xu Vincent Stoll John Jumper Amoolya H. Singh Dan Eaton Qi Hao |
| author_sort | Russell A. Judge |
| collection | DOAJ |
| description | PAPP-A substrate selectivity underlies the tight regulation of IGF signaling. Here, the authors report cryo-EM structures of dimeric PAPP-A in its substrate-free form and in complex with a peptide substrate, which combined with biochemical assays provide a mechanism for PAPP-A substrate binding and selectivity. |
| first_indexed | 2024-04-14T07:29:26Z |
| format | Article |
| id | doaj.art-b5c3045813c44acc833427452b1f8434 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-14T07:29:26Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-b5c3045813c44acc833427452b1f84342022-12-22T02:05:54ZengNature PortfolioNature Communications2041-17232022-09-0113111310.1038/s41467-022-33175-2Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognitionRussell A. Judge0Janani Sridar1Kathryn Tunyasunvunakool2Rinku Jain3John C. K. Wang4Christna Ouch5Jun Xu6Amirhossein Mafi7Aaron H. Nile8Clint Remarcik9Corey L. Smith10Crystal Ghosh11Chen Xu12Vincent Stoll13John Jumper14Amoolya H. Singh15Dan Eaton16Qi Hao17AbbVieCalico Life Sciences LLCDeepMindAbbVieCalico Life Sciences LLCDepartment of Biochemistry & Molecular Biotechnology, University of Massachusetts Chan Medical SchoolCalico Life Sciences LLCCalico Life Sciences LLCCalico Life Sciences LLCCalico Life Sciences LLCAbbVie Bioresearch CenterCalico Life Sciences LLCDepartment of Biochemistry & Molecular Biotechnology, University of Massachusetts Chan Medical SchoolAbbVieDeepMindCalico Life Sciences LLCCalico Life Sciences LLCCalico Life Sciences LLCPAPP-A substrate selectivity underlies the tight regulation of IGF signaling. Here, the authors report cryo-EM structures of dimeric PAPP-A in its substrate-free form and in complex with a peptide substrate, which combined with biochemical assays provide a mechanism for PAPP-A substrate binding and selectivity.https://doi.org/10.1038/s41467-022-33175-2 |
| spellingShingle | Russell A. Judge Janani Sridar Kathryn Tunyasunvunakool Rinku Jain John C. K. Wang Christna Ouch Jun Xu Amirhossein Mafi Aaron H. Nile Clint Remarcik Corey L. Smith Crystal Ghosh Chen Xu Vincent Stoll John Jumper Amoolya H. Singh Dan Eaton Qi Hao Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition Nature Communications |
| title | Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition |
| title_full | Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition |
| title_fullStr | Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition |
| title_full_unstemmed | Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition |
| title_short | Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition |
| title_sort | structure of the papp abp5 complex reveals mechanism of substrate recognition |
| url | https://doi.org/10.1038/s41467-022-33175-2 |
| work_keys_str_mv | AT russellajudge structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT jananisridar structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT kathryntunyasunvunakool structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT rinkujain structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT johnckwang structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT christnaouch structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT junxu structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT amirhosseinmafi structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT aaronhnile structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT clintremarcik structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT coreylsmith structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT crystalghosh structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT chenxu structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT vincentstoll structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT johnjumper structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT amoolyahsingh structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT daneaton structureofthepappabp5complexrevealsmechanismofsubstraterecognition AT qihao structureofthepappabp5complexrevealsmechanismofsubstraterecognition |