Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma
Carotid artery atherosclerosis is a main cause of stroke. Understanding atherosclerosis biology is critical in the development of targeted prevention and treatment strategies. Consequently, there is demand for advanced tools investigating atheroma pathology. We consider hybrid optoacoustic and multi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-09-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597916300222 |
| _version_ | 1818955874169782272 |
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| author | Markus Seeger Angelos Karlas Dominik Soliman Jaroslav Pelisek Vasilis Ntziachristos |
| author_facet | Markus Seeger Angelos Karlas Dominik Soliman Jaroslav Pelisek Vasilis Ntziachristos |
| author_sort | Markus Seeger |
| collection | DOAJ |
| description | Carotid artery atherosclerosis is a main cause of stroke. Understanding atherosclerosis biology is critical in the development of targeted prevention and treatment strategies. Consequently, there is demand for advanced tools investigating atheroma pathology. We consider hybrid optoacoustic and multiphoton microscopy for the integrated and complementary interrogation of plaque tissue constituents and their mutual interactions. Herein, we visualize human carotid plaque using a hybrid multimodal imaging system that combines optical resolution optoacoustic (photoacoustic) microscopy, second and third harmonic generation microscopy, and two-photon excitation fluorescence microscopy. Our data suggest more comprehensive insights in the pathophysiology of atheroma formation and destabilization, by enabling congruent visualization of structural and biological features critical for the atherosclerotic process and its acute complications, such as red blood cells and collagen. |
| first_indexed | 2024-12-20T10:44:59Z |
| format | Article |
| id | doaj.art-b5c3f90661d14984bf7c9f01aa7c0dac |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-12-20T10:44:59Z |
| publishDate | 2016-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-b5c3f90661d14984bf7c9f01aa7c0dac2022-12-21T19:43:26ZengElsevierPhotoacoustics2213-59792016-09-014310211110.1016/j.pacs.2016.07.001Multimodal optoacoustic and multiphoton microscopy of human carotid atheromaMarkus Seeger0Angelos Karlas1Dominik Soliman2Jaroslav Pelisek3Vasilis Ntziachristos4Chair for Biological Imaging, Technische Universität München, Munich, GermanyChair for Biological Imaging, Technische Universität München, Munich, GermanyChair for Biological Imaging, Technische Universität München, Munich, GermanyDepartment of Vascular and Endovascular Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, GermanyChair for Biological Imaging, Technische Universität München, Munich, GermanyCarotid artery atherosclerosis is a main cause of stroke. Understanding atherosclerosis biology is critical in the development of targeted prevention and treatment strategies. Consequently, there is demand for advanced tools investigating atheroma pathology. We consider hybrid optoacoustic and multiphoton microscopy for the integrated and complementary interrogation of plaque tissue constituents and their mutual interactions. Herein, we visualize human carotid plaque using a hybrid multimodal imaging system that combines optical resolution optoacoustic (photoacoustic) microscopy, second and third harmonic generation microscopy, and two-photon excitation fluorescence microscopy. Our data suggest more comprehensive insights in the pathophysiology of atheroma formation and destabilization, by enabling congruent visualization of structural and biological features critical for the atherosclerotic process and its acute complications, such as red blood cells and collagen.http://www.sciencedirect.com/science/article/pii/S2213597916300222Human carotid atheromaCollagenRed blood cellsMultimodal microscopyOptoacoustic microscopyPhotoacoustic microscopyNon-linear optical microscopy |
| spellingShingle | Markus Seeger Angelos Karlas Dominik Soliman Jaroslav Pelisek Vasilis Ntziachristos Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma Photoacoustics Human carotid atheroma Collagen Red blood cells Multimodal microscopy Optoacoustic microscopy Photoacoustic microscopy Non-linear optical microscopy |
| title | Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma |
| title_full | Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma |
| title_fullStr | Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma |
| title_full_unstemmed | Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma |
| title_short | Multimodal optoacoustic and multiphoton microscopy of human carotid atheroma |
| title_sort | multimodal optoacoustic and multiphoton microscopy of human carotid atheroma |
| topic | Human carotid atheroma Collagen Red blood cells Multimodal microscopy Optoacoustic microscopy Photoacoustic microscopy Non-linear optical microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2213597916300222 |
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