The effect of adoptive transferring myeloid-derived suppressor cells in ventilator-induced lung injury mice
The effects of adoptive transferring myeloid-derived suppressor cells (MDSCs) to mice with ventilator-induced lung injury (VILI) are unclear. Our objective was to investigate the effects of adoptively transferring MDSCs in VILI. The mouse model was created by introducing mechanical ventilation throu...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024016268 |
| _version_ | 1797304430033371136 |
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| author | Fangzhen Shan Fenglian Tang Yuan Liu Xiao Han Wei Wu Yanhua Tang Qingyuan Zhan Nannan Zhang |
| author_facet | Fangzhen Shan Fenglian Tang Yuan Liu Xiao Han Wei Wu Yanhua Tang Qingyuan Zhan Nannan Zhang |
| author_sort | Fangzhen Shan |
| collection | DOAJ |
| description | The effects of adoptive transferring myeloid-derived suppressor cells (MDSCs) to mice with ventilator-induced lung injury (VILI) are unclear. Our objective was to investigate the effects of adoptively transferring MDSCs in VILI. The mouse model was created by introducing mechanical ventilation through a high tidal volume of 20 ml/kg for 4 h. Inflammation-induced MDSCs (iMDSCs) were collected from the bone marrow of mice with cecal ligation and puncture. iMDSCs were administrated through retrobulbar angular vein 1 h before the mechanical ventilation. The control group was anesthetized and maintained spontaneous respiration. After the termination of mechanical ventilation, bronchoalveolar lavage fluid (BALF) and lung samples 6 h were collected. The concentrations of BALF protein, levels of inflammatory mediators, and white blood cells were all significantly decreased in mice treated with iMDSCs. Histological examinations indicated reduced lung damage after iMDSCs treatment. Moreover, adoptive transfer of iMDSCs could reduce CD4+ T-cell counts and inhibit its inflammatory cytokine secretion. iMDSCs treatment was found to had no immunostimulatory effects or cause secondary infections in mice. In conclusion, MDSCs might be a potential targeted therapy for alleviating the inflammatory response of VILI mice in a T-cell dependent manner. |
| first_indexed | 2024-03-08T00:10:25Z |
| format | Article |
| id | doaj.art-b5c589590f974032858b557c287a2946 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T00:10:25Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-b5c589590f974032858b557c287a29462024-02-17T06:41:30ZengElsevierHeliyon2405-84402024-02-01103e25595The effect of adoptive transferring myeloid-derived suppressor cells in ventilator-induced lung injury miceFangzhen Shan0Fenglian Tang1Yuan Liu2Xiao Han3Wei Wu4Yanhua Tang5Qingyuan Zhan6Nannan Zhang7Department of Pulmonary and Critical Care Medicine, Affiliated Hospital of Jining Medical University, Shandong, China; Medical Research Center, Affiliated Hospital of Jining Medical University, Shandong, ChinaDepartment of Pulmonary and Critical Care Medicine, Affiliated Hospital of Jining Medical University, Shandong, ChinaDepartment of Intensive care unit III, Affiliated Hospital of Jining Medical University, Shandong, ChinaDepartment of Pulmonary and Critical Care Medicine, Affiliated Hospital of Jining Medical University, Shandong, ChinaDepartment of Pulmonary and Critical Care Medicine, Affiliated Hospital of Jining Medical University, Shandong, ChinaDepartment of Pulmonary and Critical Care Medicine, Affiliated Hospital of Jining Medical University, Shandong, ChinaDepartment of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, ChinaDepartment of Pulmonary and Critical Care Medicine, Affiliated Hospital of Jining Medical University, Shandong, China; Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, China; Corresponding author. Department of Pulmonary and Critical Care Medicine, Affiliated Hospital of Jining Medical University, 272029, Shandong, China; Department of Pulmonary and Critical Care Medicine, China-Japan Friendship Hospital, Beijing, China.The effects of adoptive transferring myeloid-derived suppressor cells (MDSCs) to mice with ventilator-induced lung injury (VILI) are unclear. Our objective was to investigate the effects of adoptively transferring MDSCs in VILI. The mouse model was created by introducing mechanical ventilation through a high tidal volume of 20 ml/kg for 4 h. Inflammation-induced MDSCs (iMDSCs) were collected from the bone marrow of mice with cecal ligation and puncture. iMDSCs were administrated through retrobulbar angular vein 1 h before the mechanical ventilation. The control group was anesthetized and maintained spontaneous respiration. After the termination of mechanical ventilation, bronchoalveolar lavage fluid (BALF) and lung samples 6 h were collected. The concentrations of BALF protein, levels of inflammatory mediators, and white blood cells were all significantly decreased in mice treated with iMDSCs. Histological examinations indicated reduced lung damage after iMDSCs treatment. Moreover, adoptive transfer of iMDSCs could reduce CD4+ T-cell counts and inhibit its inflammatory cytokine secretion. iMDSCs treatment was found to had no immunostimulatory effects or cause secondary infections in mice. In conclusion, MDSCs might be a potential targeted therapy for alleviating the inflammatory response of VILI mice in a T-cell dependent manner.http://www.sciencedirect.com/science/article/pii/S2405844024016268Ventilator-induced lung injuryMyeloid-derived suppressor cellsInflammationT cells |
| spellingShingle | Fangzhen Shan Fenglian Tang Yuan Liu Xiao Han Wei Wu Yanhua Tang Qingyuan Zhan Nannan Zhang The effect of adoptive transferring myeloid-derived suppressor cells in ventilator-induced lung injury mice Heliyon Ventilator-induced lung injury Myeloid-derived suppressor cells Inflammation T cells |
| title | The effect of adoptive transferring myeloid-derived suppressor cells in ventilator-induced lung injury mice |
| title_full | The effect of adoptive transferring myeloid-derived suppressor cells in ventilator-induced lung injury mice |
| title_fullStr | The effect of adoptive transferring myeloid-derived suppressor cells in ventilator-induced lung injury mice |
| title_full_unstemmed | The effect of adoptive transferring myeloid-derived suppressor cells in ventilator-induced lung injury mice |
| title_short | The effect of adoptive transferring myeloid-derived suppressor cells in ventilator-induced lung injury mice |
| title_sort | effect of adoptive transferring myeloid derived suppressor cells in ventilator induced lung injury mice |
| topic | Ventilator-induced lung injury Myeloid-derived suppressor cells Inflammation T cells |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024016268 |
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