Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (<i>Camellia sinensis</i> (L.) Kuntze)
<i>C. sinensis</i> is an economically important crop for tea production that experiences increasing demand and good export potential. Therefore, crops need to be expanded, and high-quality planting material is required. Vegetative propagation by cuttings is the prevalent method; therefor...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/6/1561 |
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| author | Yaozong Shen Hui Wang Xiao Han Kai Fan Jiazhi Shen He Li Shibo Ding Dapeng Song Yu Wang Zhaotang Ding |
| author_facet | Yaozong Shen Hui Wang Xiao Han Kai Fan Jiazhi Shen He Li Shibo Ding Dapeng Song Yu Wang Zhaotang Ding |
| author_sort | Yaozong Shen |
| collection | DOAJ |
| description | <i>C. sinensis</i> is an economically important crop for tea production that experiences increasing demand and good export potential. Therefore, crops need to be expanded, and high-quality planting material is required. Vegetative propagation by cuttings is the prevalent method; therefore, this paper explored its optimization potential modeled on cultivar ‘Jiukengzao’. This study wanted to deeply explore blue-light-induced adventitious root formation and development of tea cuttings, so we conducted short-term (0 h, 8 h, and 16 h) and long-term (30 d, 60 d, and 90 d) time-course analyses on tea cutting seedlings. Short-term, full-length transcriptome analysis showed that the expression of genes related to plant hormone signal transduction and auxin transport was highest at 16 h. Sixteen hours of light was considered as suitable for adventitious root growth and development of tea cuttings. Long-term phytohormone analysis showed that the trend of indole-3-carboxylic acid (ICA) change was: 60 d > 90 d > 30 d. Long-term, full-length transcriptome analysis showed that the gene expression trends in K2, K5, K6, and K8 clusters were: 90 d > 60 d > 30 d, and the opposite was observed in K1, K4, and K11 clusters. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most of the genes in these seven clusters are involved in “plant hormone signal transduction (ko04075)”. This includes auxin early responsive protein <i>AUX/IAA</i>, auxin response factor <i>ARF</i>, auxin-responsive protein <i>SAUR</i>, etc. In addition, genes related to auxin transport and synthesis were identified as <i>PIN1</i>, <i>3</i>, <i>4</i>, <i>PILS2</i>, <i>6</i>, <i>7</i>, flavin-containing monooxygenase <i>YUC9</i>, and <i>YUC10</i>, and the expression trend of these genes was mostly consistent with the change trend of ICA content. This study further explained the molecular mechanism of blue-light-induced adventitious root formation and development of tea cuttings. It is recommended that blue light can be used to promote the adventitious root growth and development of tea cuttings in practical production. |
| first_indexed | 2024-03-11T02:52:03Z |
| format | Article |
| id | doaj.art-b5c7122e3ec14fa3980a09cc4d2940ec |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T02:52:03Z |
| publishDate | 2023-06-01 |
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| series | Agronomy |
| spelling | doaj.art-b5c7122e3ec14fa3980a09cc4d2940ec2023-11-18T08:54:56ZengMDPI AGAgronomy2073-43952023-06-01136156110.3390/agronomy13061561Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (<i>Camellia sinensis</i> (L.) Kuntze)Yaozong Shen0Hui Wang1Xiao Han2Kai Fan3Jiazhi Shen4He Li5Shibo Ding6Dapeng Song7Yu Wang8Zhaotang Ding9Tea Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaTea Research Institute, Rizhao Academy of Agricultural Sciences, Rizhao 276800, ChinaTea Research Institute, Qingdao Agricultural University, Qingdao 266109, ChinaTea Research Institute, Qingdao Agricultural University, Qingdao 266109, ChinaTea Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaTea Research Institute, Qingdao Agricultural University, Qingdao 266109, ChinaTea Research Institute, Rizhao Academy of Agricultural Sciences, Rizhao 276800, ChinaTea Research Institute, Rizhao Academy of Agricultural Sciences, Rizhao 276800, ChinaTea Research Institute, Qingdao Agricultural University, Qingdao 266109, ChinaTea Research Institute, Shandong Academy of Agricultural Sciences, Jinan 250100, China<i>C. sinensis</i> is an economically important crop for tea production that experiences increasing demand and good export potential. Therefore, crops need to be expanded, and high-quality planting material is required. Vegetative propagation by cuttings is the prevalent method; therefore, this paper explored its optimization potential modeled on cultivar ‘Jiukengzao’. This study wanted to deeply explore blue-light-induced adventitious root formation and development of tea cuttings, so we conducted short-term (0 h, 8 h, and 16 h) and long-term (30 d, 60 d, and 90 d) time-course analyses on tea cutting seedlings. Short-term, full-length transcriptome analysis showed that the expression of genes related to plant hormone signal transduction and auxin transport was highest at 16 h. Sixteen hours of light was considered as suitable for adventitious root growth and development of tea cuttings. Long-term phytohormone analysis showed that the trend of indole-3-carboxylic acid (ICA) change was: 60 d > 90 d > 30 d. Long-term, full-length transcriptome analysis showed that the gene expression trends in K2, K5, K6, and K8 clusters were: 90 d > 60 d > 30 d, and the opposite was observed in K1, K4, and K11 clusters. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most of the genes in these seven clusters are involved in “plant hormone signal transduction (ko04075)”. This includes auxin early responsive protein <i>AUX/IAA</i>, auxin response factor <i>ARF</i>, auxin-responsive protein <i>SAUR</i>, etc. In addition, genes related to auxin transport and synthesis were identified as <i>PIN1</i>, <i>3</i>, <i>4</i>, <i>PILS2</i>, <i>6</i>, <i>7</i>, flavin-containing monooxygenase <i>YUC9</i>, and <i>YUC10</i>, and the expression trend of these genes was mostly consistent with the change trend of ICA content. This study further explained the molecular mechanism of blue-light-induced adventitious root formation and development of tea cuttings. It is recommended that blue light can be used to promote the adventitious root growth and development of tea cuttings in practical production.https://www.mdpi.com/2073-4395/13/6/1561tea cuttingblue lighttime-course analysisfull-length transcriptomek-meansphytohormone |
| spellingShingle | Yaozong Shen Hui Wang Xiao Han Kai Fan Jiazhi Shen He Li Shibo Ding Dapeng Song Yu Wang Zhaotang Ding Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (<i>Camellia sinensis</i> (L.) Kuntze) Agronomy tea cutting blue light time-course analysis full-length transcriptome k-means phytohormone |
| title | Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (<i>Camellia sinensis</i> (L.) Kuntze) |
| title_full | Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (<i>Camellia sinensis</i> (L.) Kuntze) |
| title_fullStr | Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (<i>Camellia sinensis</i> (L.) Kuntze) |
| title_full_unstemmed | Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (<i>Camellia sinensis</i> (L.) Kuntze) |
| title_short | Time-Course Transcriptome and Phytohormonal Analysis of Blue-Light-Induced Adventitious Root Development of Tea Cuttings (<i>Camellia sinensis</i> (L.) Kuntze) |
| title_sort | time course transcriptome and phytohormonal analysis of blue light induced adventitious root development of tea cuttings i camellia sinensis i l kuntze |
| topic | tea cutting blue light time-course analysis full-length transcriptome k-means phytohormone |
| url | https://www.mdpi.com/2073-4395/13/6/1561 |
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