Carcinogenicity and testicular toxicity of 2-bromopropane in a 26-week inhalation study using the rasH2 mouse model
Abstract 2-Bromopropane (2-BP) is a colorless liquid at room temperature and is used in closed systems in factories, mainly as an intermediate for medicines, pesticides, and other chemicals. However, the carcinogenicity of 2-BP is still unknown. The CByB6F1-Tg(HRAS)2Jic (rasH2) transgenic mouse mode...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-28825-4 |
| _version_ | 1811171717098242048 |
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| author | Yuko Goto Arata Saito Kenji Takanobu Hideki Senoh Misae Saito Yumi Umeda Shotaro Yamano |
| author_facet | Yuko Goto Arata Saito Kenji Takanobu Hideki Senoh Misae Saito Yumi Umeda Shotaro Yamano |
| author_sort | Yuko Goto |
| collection | DOAJ |
| description | Abstract 2-Bromopropane (2-BP) is a colorless liquid at room temperature and is used in closed systems in factories, mainly as an intermediate for medicines, pesticides, and other chemicals. However, the carcinogenicity of 2-BP is still unknown. The CByB6F1-Tg(HRAS)2Jic (rasH2) transgenic mouse model has been established as an alternative to long-term studies (1.5 years—lifetime) to detect carcinogenicity in as short a time as six months. We performed a 26-week inhalation exposure study of 2-BP using the rasH2 mouse model. Male and female rasH2 mice were exposed to 0, 67, 200, or 600 ppm of 2-BP for 6 h/day, 5 days/week for 26 weeks. All tissues and blood were collected and subjected to biological and histopathological analyses. The results showed a concentration-dependent increase in lung tumor development in male and female rasH2 mice exposed by inhalation to 2-BP, which was significant by Peto’s and Poly-3 trend tests. Furthermore, in male rasH2 mice, 2-BP was found to be a testicular toxin. This study is the first to demonstrate that 2-BP is carcinogenic in male and female mice and a testicular toxin in male mice using the rasH2 mouse model. |
| first_indexed | 2024-04-10T17:19:44Z |
| format | Article |
| id | doaj.art-b5c9e7de3ff0491495302d56f9aa4575 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T17:19:44Z |
| publishDate | 2023-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj.art-b5c9e7de3ff0491495302d56f9aa45752023-02-05T12:12:18ZengNature PortfolioScientific Reports2045-23222023-01-0113111410.1038/s41598-023-28825-4Carcinogenicity and testicular toxicity of 2-bromopropane in a 26-week inhalation study using the rasH2 mouse modelYuko Goto0Arata Saito1Kenji Takanobu2Hideki Senoh3Misae Saito4Yumi Umeda5Shotaro Yamano6Japan Bioassay Research Center, Japan Organization of Occupational Health and SafetyJapan Bioassay Research Center, Japan Organization of Occupational Health and SafetyJapan Bioassay Research Center, Japan Organization of Occupational Health and SafetyJapan Bioassay Research Center, Japan Organization of Occupational Health and SafetyJapan Bioassay Research Center, Japan Organization of Occupational Health and SafetyJapan Bioassay Research Center, Japan Organization of Occupational Health and SafetyJapan Bioassay Research Center, Japan Organization of Occupational Health and SafetyAbstract 2-Bromopropane (2-BP) is a colorless liquid at room temperature and is used in closed systems in factories, mainly as an intermediate for medicines, pesticides, and other chemicals. However, the carcinogenicity of 2-BP is still unknown. The CByB6F1-Tg(HRAS)2Jic (rasH2) transgenic mouse model has been established as an alternative to long-term studies (1.5 years—lifetime) to detect carcinogenicity in as short a time as six months. We performed a 26-week inhalation exposure study of 2-BP using the rasH2 mouse model. Male and female rasH2 mice were exposed to 0, 67, 200, or 600 ppm of 2-BP for 6 h/day, 5 days/week for 26 weeks. All tissues and blood were collected and subjected to biological and histopathological analyses. The results showed a concentration-dependent increase in lung tumor development in male and female rasH2 mice exposed by inhalation to 2-BP, which was significant by Peto’s and Poly-3 trend tests. Furthermore, in male rasH2 mice, 2-BP was found to be a testicular toxin. This study is the first to demonstrate that 2-BP is carcinogenic in male and female mice and a testicular toxin in male mice using the rasH2 mouse model.https://doi.org/10.1038/s41598-023-28825-4 |
| spellingShingle | Yuko Goto Arata Saito Kenji Takanobu Hideki Senoh Misae Saito Yumi Umeda Shotaro Yamano Carcinogenicity and testicular toxicity of 2-bromopropane in a 26-week inhalation study using the rasH2 mouse model Scientific Reports |
| title | Carcinogenicity and testicular toxicity of 2-bromopropane in a 26-week inhalation study using the rasH2 mouse model |
| title_full | Carcinogenicity and testicular toxicity of 2-bromopropane in a 26-week inhalation study using the rasH2 mouse model |
| title_fullStr | Carcinogenicity and testicular toxicity of 2-bromopropane in a 26-week inhalation study using the rasH2 mouse model |
| title_full_unstemmed | Carcinogenicity and testicular toxicity of 2-bromopropane in a 26-week inhalation study using the rasH2 mouse model |
| title_short | Carcinogenicity and testicular toxicity of 2-bromopropane in a 26-week inhalation study using the rasH2 mouse model |
| title_sort | carcinogenicity and testicular toxicity of 2 bromopropane in a 26 week inhalation study using the rash2 mouse model |
| url | https://doi.org/10.1038/s41598-023-28825-4 |
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