A decreased abundance of clostridia characterizes the gut microbiota in eosinophilic esophagitis
Abstract Abnormalities in the gut microbiome are associated with suppressed Th2 response (Belizario et al., 2018 Mediators Inflamm. 2018:2037838) and predisposition to atopic disease such as asthma and eczema. We investigated if this applies to eosinophilic esophagitis (EoE). Stool bacterial DNA was...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.14261 |
| _version_ | 1828397170621939712 |
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| author | Purna C. Kashyap Stephen Johnson Debra M. Geno Heather R. Lekatz Crystal Lavey Jeffrey A. Alexander Jun Chen David A. Katzka |
| author_facet | Purna C. Kashyap Stephen Johnson Debra M. Geno Heather R. Lekatz Crystal Lavey Jeffrey A. Alexander Jun Chen David A. Katzka |
| author_sort | Purna C. Kashyap |
| collection | DOAJ |
| description | Abstract Abnormalities in the gut microbiome are associated with suppressed Th2 response (Belizario et al., 2018 Mediators Inflamm. 2018:2037838) and predisposition to atopic disease such as asthma and eczema. We investigated if this applies to eosinophilic esophagitis (EoE). Stool bacterial DNA was extracted and followed by 16S rRNA amplification from 12 patients with eosinophilic esophagitis and 12 controls. Alpha‐ and beta‐diversity were analyzed. Only two patients had asthma or atopy and one patient was on budesonide. No patients were on PPIs. Patients with EoE had lower gut microbiota alpha diversity (species richness, P = 0.09; Shannon index, P = 0.01). The microbial composition was distinct as evidenced by significantly different beta diversity (P = 0.03) when compared to healthy controls. There were also significant differences in relative abundance at multiple taxonomic levels when comparing the two communities; at the phylum level, we observed a marked decrease in Firmicutes and increase in Bacteroidetes and at the order and family level there were significant decreases in Clostridia and Clostridiales in patients with EoE (q ≤ 0.1). We conclude that there are significant differences in microbial community structure, microbial richness, and evenness and a significant decrease in taxa within the Clostridia in patients with EoE. Our data suggest that Clostridia based interventions could be tested as adjuncts to current therapeutic strategies in EoE. |
| first_indexed | 2024-12-10T08:41:58Z |
| format | Article |
| id | doaj.art-b5ca12b56bea457bbc37bed674779bd7 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-10T08:41:58Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-b5ca12b56bea457bbc37bed674779bd72022-12-22T01:55:49ZengWileyPhysiological Reports2051-817X2019-10-01720n/an/a10.14814/phy2.14261A decreased abundance of clostridia characterizes the gut microbiota in eosinophilic esophagitisPurna C. Kashyap0Stephen Johnson1Debra M. Geno2Heather R. Lekatz3Crystal Lavey4Jeffrey A. Alexander5Jun Chen6David A. Katzka7Gastroenterology and Hepatology Mayo Clinic Rochester MinnesotaBiomedical Statistics and Informatics Mayo Clinic Rochester MinnesotaGastroenterology and Hepatology Mayo Clinic Rochester MinnesotaGastroenterology and Hepatology Mayo Clinic Rochester MinnesotaGastroenterology and Hepatology Mayo Clinic Rochester MinnesotaGastroenterology and Hepatology Mayo Clinic Rochester MinnesotaPhysiology and Biomedical Engineering Mayo Clinic Rochester MinnesotaGastroenterology and Hepatology Mayo Clinic Rochester MinnesotaAbstract Abnormalities in the gut microbiome are associated with suppressed Th2 response (Belizario et al., 2018 Mediators Inflamm. 2018:2037838) and predisposition to atopic disease such as asthma and eczema. We investigated if this applies to eosinophilic esophagitis (EoE). Stool bacterial DNA was extracted and followed by 16S rRNA amplification from 12 patients with eosinophilic esophagitis and 12 controls. Alpha‐ and beta‐diversity were analyzed. Only two patients had asthma or atopy and one patient was on budesonide. No patients were on PPIs. Patients with EoE had lower gut microbiota alpha diversity (species richness, P = 0.09; Shannon index, P = 0.01). The microbial composition was distinct as evidenced by significantly different beta diversity (P = 0.03) when compared to healthy controls. There were also significant differences in relative abundance at multiple taxonomic levels when comparing the two communities; at the phylum level, we observed a marked decrease in Firmicutes and increase in Bacteroidetes and at the order and family level there were significant decreases in Clostridia and Clostridiales in patients with EoE (q ≤ 0.1). We conclude that there are significant differences in microbial community structure, microbial richness, and evenness and a significant decrease in taxa within the Clostridia in patients with EoE. Our data suggest that Clostridia based interventions could be tested as adjuncts to current therapeutic strategies in EoE.https://doi.org/10.14814/phy2.14261Microbiomeeosinophilic esophagitisallergy |
| spellingShingle | Purna C. Kashyap Stephen Johnson Debra M. Geno Heather R. Lekatz Crystal Lavey Jeffrey A. Alexander Jun Chen David A. Katzka A decreased abundance of clostridia characterizes the gut microbiota in eosinophilic esophagitis Physiological Reports Microbiome eosinophilic esophagitis allergy |
| title | A decreased abundance of clostridia characterizes the gut microbiota in eosinophilic esophagitis |
| title_full | A decreased abundance of clostridia characterizes the gut microbiota in eosinophilic esophagitis |
| title_fullStr | A decreased abundance of clostridia characterizes the gut microbiota in eosinophilic esophagitis |
| title_full_unstemmed | A decreased abundance of clostridia characterizes the gut microbiota in eosinophilic esophagitis |
| title_short | A decreased abundance of clostridia characterizes the gut microbiota in eosinophilic esophagitis |
| title_sort | decreased abundance of clostridia characterizes the gut microbiota in eosinophilic esophagitis |
| topic | Microbiome eosinophilic esophagitis allergy |
| url | https://doi.org/10.14814/phy2.14261 |
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