House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis Endotype
Atopic dermatitis (AD) endotyping might be important for developing personalized diagnostic and therapeutic strategies to the different phenotypes. The current study investigated the IgE molecular profile to <i>Dermatophagoides pteronyssinus</i> (<i>D. pteronyssinus</i>) in a...
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MDPI AG
2021-12-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/12/1418 |
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| author | Ruperto González-Pérez Paloma Poza-Guedes Fernando Pineda Miriam Castillo Inmaculada Sánchez-Machín |
| author_facet | Ruperto González-Pérez Paloma Poza-Guedes Fernando Pineda Miriam Castillo Inmaculada Sánchez-Machín |
| author_sort | Ruperto González-Pérez |
| collection | DOAJ |
| description | Atopic dermatitis (AD) endotyping might be important for developing personalized diagnostic and therapeutic strategies to the different phenotypes. The current study investigated the IgE molecular profile to <i>Dermatophagoides pteronyssinus</i> (<i>D. pteronyssinus</i>) in a subset of patients afflicted with varying severity stages of atopic dermatitis in a subtropical region subjected to a high perennial house dust mite (HDM) exposure. We selected patients showing a clinically relevant sensitization to HDM with mild-to-moderate and severe AD according to their basal Severity Scoring Atopic Dermatitis (SCORAD) index. Skin prick test (SPT) with standardized mite extracts, as well as a Precision Allergy Molecular Diagnosis (PAMD@) panel including nine different <i>D. </i><i>pteronyssinus</i> allergens and the related protein allergenic characterization, were assessed in all serum samples. A total of 80 European American AD patients with the marked T2 endotype confirmed their eligibility for the study. Major allergens (Der p 23, Der p 2, and Der p 1) were present in more than 86% of all subjects, with mid-tier allergens (Der p 5, Der p 7, and Der p 21) reaching up to 65%. A serodominant role for Der p 11 could not be quantitatively confirmed in the present cohort. The proposed component resolved diagnosis (CRD) panel appeared to be sufficient to obtain a precise <i>D. pteronyssinus</i> molecular diagnosis in AD patients subjected to a climate-dependent high-mite allergen exposure. The raised seroprevalence of IgE response to Der p 23 confirmed this constituent as a major <i>D. pteronyssinus</i> allergen in severe stages of atopic dermatitis. A clinically driven molecular approach appears to be essential to frame a more precise diagnosis and therapy of this heterogeneous allergic condition. |
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| id | doaj.art-b5cc9954604a41aa83865285a7965750 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T03:43:17Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-b5cc9954604a41aa83865285a79657502023-11-23T09:15:11ZengMDPI AGLife2075-17292021-12-011112141810.3390/life11121418House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis EndotypeRuperto González-Pérez0Paloma Poza-Guedes1Fernando Pineda2Miriam Castillo3Inmaculada Sánchez-Machín4Allergy Department, Hospital Universitario de Canarias, 38320 Tenerife, SpainAllergy Department, Hospital Universitario de Canarias, 38320 Tenerife, SpainDiater Laboratories, 28191 Madrid, SpainDiater Laboratories, 28191 Madrid, SpainAllergy Department, Hospital Universitario de Canarias, 38320 Tenerife, SpainAtopic dermatitis (AD) endotyping might be important for developing personalized diagnostic and therapeutic strategies to the different phenotypes. The current study investigated the IgE molecular profile to <i>Dermatophagoides pteronyssinus</i> (<i>D. pteronyssinus</i>) in a subset of patients afflicted with varying severity stages of atopic dermatitis in a subtropical region subjected to a high perennial house dust mite (HDM) exposure. We selected patients showing a clinically relevant sensitization to HDM with mild-to-moderate and severe AD according to their basal Severity Scoring Atopic Dermatitis (SCORAD) index. Skin prick test (SPT) with standardized mite extracts, as well as a Precision Allergy Molecular Diagnosis (PAMD@) panel including nine different <i>D. </i><i>pteronyssinus</i> allergens and the related protein allergenic characterization, were assessed in all serum samples. A total of 80 European American AD patients with the marked T2 endotype confirmed their eligibility for the study. Major allergens (Der p 23, Der p 2, and Der p 1) were present in more than 86% of all subjects, with mid-tier allergens (Der p 5, Der p 7, and Der p 21) reaching up to 65%. A serodominant role for Der p 11 could not be quantitatively confirmed in the present cohort. The proposed component resolved diagnosis (CRD) panel appeared to be sufficient to obtain a precise <i>D. pteronyssinus</i> molecular diagnosis in AD patients subjected to a climate-dependent high-mite allergen exposure. The raised seroprevalence of IgE response to Der p 23 confirmed this constituent as a major <i>D. pteronyssinus</i> allergen in severe stages of atopic dermatitis. A clinically driven molecular approach appears to be essential to frame a more precise diagnosis and therapy of this heterogeneous allergic condition.https://www.mdpi.com/2075-1729/11/12/1418allergyatopic dermatitisallergen<i>Dermatophagoides pteronyssinus</i>endotypeprecision allergy molecular diagnosis |
| spellingShingle | Ruperto González-Pérez Paloma Poza-Guedes Fernando Pineda Miriam Castillo Inmaculada Sánchez-Machín House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis Endotype Life allergy atopic dermatitis allergen <i>Dermatophagoides pteronyssinus</i> endotype precision allergy molecular diagnosis |
| title | House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis Endotype |
| title_full | House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis Endotype |
| title_fullStr | House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis Endotype |
| title_full_unstemmed | House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis Endotype |
| title_short | House Dust Mite Precision Allergy Molecular Diagnosis (PAMD@) in the Th2-prone Atopic Dermatitis Endotype |
| title_sort | house dust mite precision allergy molecular diagnosis pamd in the th2 prone atopic dermatitis endotype |
| topic | allergy atopic dermatitis allergen <i>Dermatophagoides pteronyssinus</i> endotype precision allergy molecular diagnosis |
| url | https://www.mdpi.com/2075-1729/11/12/1418 |
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