Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolation
<abstract language="eng">The aim of this study was to compare three different selective enrichment broths: Rappaport-Vassiliadis (RV), selenite cystine (SC) and Muller-Kauffmann tetrathionate (MKT) for Salmonella Dublin isolation from faecal samples of calf experimentally infected. T...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
2008-06-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300036 |
| _version_ | 1818254896436084736 |
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| author | D.G. Silva J.J. Fagliari T.B. Garcia |
| author_facet | D.G. Silva J.J. Fagliari T.B. Garcia |
| author_sort | D.G. Silva |
| collection | DOAJ |
| description | <abstract language="eng">The aim of this study was to compare three different selective enrichment broths: Rappaport-Vassiliadis (RV), selenite cystine (SC) and Muller-Kauffmann tetrathionate (MKT) for Salmonella Dublin isolation from faecal samples of calf experimentally infected. The bacteriological procedure involved pre-enrichment stages in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment, culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antiserum. The effects of enrichment temperatures using RV broth were also evaluated (37ºC and 42ºC). SC broth was significantly more efficient in the isolation of Salmonella Dublin (P<0,05), whereas RV broth incubated at 42ºC had a lower efficiency in the microbiological isolation. |
| first_indexed | 2024-12-12T17:03:15Z |
| format | Article |
| id | doaj.art-b5cf0a519e6442ec87083a06a2cfd70f |
| institution | Directory Open Access Journal |
| issn | 0102-0935 1678-4162 |
| language | English |
| last_indexed | 2024-12-12T17:03:15Z |
| publishDate | 2008-06-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-b5cf0a519e6442ec87083a06a2cfd70f2022-12-22T00:18:05ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia0102-09351678-41622008-06-0160376676810.1590/S0102-09352008000300036Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolationD.G. SilvaJ.J. FagliariT.B. Garcia<abstract language="eng">The aim of this study was to compare three different selective enrichment broths: Rappaport-Vassiliadis (RV), selenite cystine (SC) and Muller-Kauffmann tetrathionate (MKT) for Salmonella Dublin isolation from faecal samples of calf experimentally infected. The bacteriological procedure involved pre-enrichment stages in Hajna-GN broth (only for the samples inoculated in RV broth), selective enrichment, culture in modified brilliant green agar (BGA), presumptive biochemistry tests (using triple-sugar-iron agar and lysine-agar) and slide agglutination test with poli-O and poli-H Salmonella antiserum. The effects of enrichment temperatures using RV broth were also evaluated (37ºC and 42ºC). SC broth was significantly more efficient in the isolation of Salmonella Dublin (P<0,05), whereas RV broth incubated at 42ºC had a lower efficiency in the microbiological isolation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300036bezerroSalmonella Dublinmeio seletivofezesSalmonella Dublinselective enrichmentcalvesfeces |
| spellingShingle | D.G. Silva J.J. Fagliari T.B. Garcia Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolation Arquivo Brasileiro de Medicina Veterinária e Zootecnia bezerro Salmonella Dublin meio seletivo fezes Salmonella Dublin selective enrichment calves feces |
| title | Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolation |
| title_full | Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolation |
| title_fullStr | Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolation |
| title_full_unstemmed | Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolation |
| title_short | Comparação da eficiência dos caldos de enriquecimento seletivo no isolamento de Salmonella Dublin Comparison of the efficiency of selective enrichment broths for Salmonella Dublin isolation |
| title_sort | comparacao da eficiencia dos caldos de enriquecimento seletivo no isolamento de salmonella dublin comparison of the efficiency of selective enrichment broths for salmonella dublin isolation |
| topic | bezerro Salmonella Dublin meio seletivo fezes Salmonella Dublin selective enrichment calves feces |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300036 |
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