Chronic Nicotine Exposure Increases Hematoma Expansion following Collagenase-Induced Intracerebral Hemorrhage in Rats
Spontaneous intracerebral hemorrhage (sICH) is a deadly stroke subtype, and tobacco use increases sICH risk. However epidemiological studies show that, there are no confirmatory studies showing the effect of tobacco use on sICH outcome. Therefore, we evaluated the effect of chronic nicotine exposure...
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MDPI AG
2022-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/5/621 |
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| author | Ashish K. Rehni Sunjoo Cho Zhexuan Zhang Weizhao Zhao Ami P. Raval Miguel A. Perez-Pinzon Kunjan R. Dave |
| author_facet | Ashish K. Rehni Sunjoo Cho Zhexuan Zhang Weizhao Zhao Ami P. Raval Miguel A. Perez-Pinzon Kunjan R. Dave |
| author_sort | Ashish K. Rehni |
| collection | DOAJ |
| description | Spontaneous intracerebral hemorrhage (sICH) is a deadly stroke subtype, and tobacco use increases sICH risk. However epidemiological studies show that, there are no confirmatory studies showing the effect of tobacco use on sICH outcome. Therefore, we evaluated the effect of chronic nicotine exposure (as a surrogate for tobacco use) on outcomes following sICH. Young male and female rats were randomly assigned to either nicotine (4.5 mg/kg b.w. per day) or vehicle (saline) treatment (2–3 weeks) groups. sICH was induced by injecting collagenase into the right striatum. Neurological score and hematoma volume were determined 24 h post-sICH. The hematoma volumes in nicotine-treated male and female rats were significantly higher by 42% and 48% when compared to vehicle-treated male and female rats, respectively. Neurological deficits measured in terms of neurological score for the nicotine-treated male and female groups were significantly higher when compared to the respective vehicle-treated male and female groups. Our results show that chronic nicotine exposure increases hematoma volume post-sICH in rats of both sexes. Identifying the mechanism of nicotine-dependent increase in hematoma growth post-sICH will be crucial to understanding the detrimental effect of tobacco use on the severity of bleeding following intracerebral hemorrhage. |
| first_indexed | 2024-03-10T03:16:48Z |
| format | Article |
| id | doaj.art-b5d282e95f4843328a861cf6c12c066d |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T03:16:48Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-b5d282e95f4843328a861cf6c12c066d2023-11-23T10:13:05ZengMDPI AGBiomolecules2218-273X2022-04-0112562110.3390/biom12050621Chronic Nicotine Exposure Increases Hematoma Expansion following Collagenase-Induced Intracerebral Hemorrhage in RatsAshish K. Rehni0Sunjoo Cho1Zhexuan Zhang2Weizhao Zhao3Ami P. Raval4Miguel A. Perez-Pinzon5Kunjan R. Dave6Peritz Scheinberg Cerebral Vascular Disease Research Laboratory, Department of Neurology, Leonard M. Miller School of Medicine, University of Miami, Neurology Research Building, 1420 NW 9th Avenue, Room # 203, Miami, FL 33136, USAPeritz Scheinberg Cerebral Vascular Disease Research Laboratory, Department of Neurology, Leonard M. Miller School of Medicine, University of Miami, Neurology Research Building, 1420 NW 9th Avenue, Room # 203, Miami, FL 33136, USADepartment of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, USADepartment of Biomedical Engineering, University of Miami, Coral Gables, FL 33146, USAPeritz Scheinberg Cerebral Vascular Disease Research Laboratory, Department of Neurology, Leonard M. Miller School of Medicine, University of Miami, Neurology Research Building, 1420 NW 9th Avenue, Room # 203, Miami, FL 33136, USAPeritz Scheinberg Cerebral Vascular Disease Research Laboratory, Department of Neurology, Leonard M. Miller School of Medicine, University of Miami, Neurology Research Building, 1420 NW 9th Avenue, Room # 203, Miami, FL 33136, USAPeritz Scheinberg Cerebral Vascular Disease Research Laboratory, Department of Neurology, Leonard M. Miller School of Medicine, University of Miami, Neurology Research Building, 1420 NW 9th Avenue, Room # 203, Miami, FL 33136, USASpontaneous intracerebral hemorrhage (sICH) is a deadly stroke subtype, and tobacco use increases sICH risk. However epidemiological studies show that, there are no confirmatory studies showing the effect of tobacco use on sICH outcome. Therefore, we evaluated the effect of chronic nicotine exposure (as a surrogate for tobacco use) on outcomes following sICH. Young male and female rats were randomly assigned to either nicotine (4.5 mg/kg b.w. per day) or vehicle (saline) treatment (2–3 weeks) groups. sICH was induced by injecting collagenase into the right striatum. Neurological score and hematoma volume were determined 24 h post-sICH. The hematoma volumes in nicotine-treated male and female rats were significantly higher by 42% and 48% when compared to vehicle-treated male and female rats, respectively. Neurological deficits measured in terms of neurological score for the nicotine-treated male and female groups were significantly higher when compared to the respective vehicle-treated male and female groups. Our results show that chronic nicotine exposure increases hematoma volume post-sICH in rats of both sexes. Identifying the mechanism of nicotine-dependent increase in hematoma growth post-sICH will be crucial to understanding the detrimental effect of tobacco use on the severity of bleeding following intracerebral hemorrhage.https://www.mdpi.com/2218-273X/12/5/621cigarettetobaccosmokingrisk factorstroke |
| spellingShingle | Ashish K. Rehni Sunjoo Cho Zhexuan Zhang Weizhao Zhao Ami P. Raval Miguel A. Perez-Pinzon Kunjan R. Dave Chronic Nicotine Exposure Increases Hematoma Expansion following Collagenase-Induced Intracerebral Hemorrhage in Rats Biomolecules cigarette tobacco smoking risk factor stroke |
| title | Chronic Nicotine Exposure Increases Hematoma Expansion following Collagenase-Induced Intracerebral Hemorrhage in Rats |
| title_full | Chronic Nicotine Exposure Increases Hematoma Expansion following Collagenase-Induced Intracerebral Hemorrhage in Rats |
| title_fullStr | Chronic Nicotine Exposure Increases Hematoma Expansion following Collagenase-Induced Intracerebral Hemorrhage in Rats |
| title_full_unstemmed | Chronic Nicotine Exposure Increases Hematoma Expansion following Collagenase-Induced Intracerebral Hemorrhage in Rats |
| title_short | Chronic Nicotine Exposure Increases Hematoma Expansion following Collagenase-Induced Intracerebral Hemorrhage in Rats |
| title_sort | chronic nicotine exposure increases hematoma expansion following collagenase induced intracerebral hemorrhage in rats |
| topic | cigarette tobacco smoking risk factor stroke |
| url | https://www.mdpi.com/2218-273X/12/5/621 |
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