Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery
Novel alginate-hydroxyapatite hybrid microspheres were developed for simultaneous delivery of drugs and cells as a multifunctional bone substitute for osteoporotic bone tissue regeneration. The microspheres were used to enhance osteogenesis and to carry and deliver quercetin, a representative phytoe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.827626/full |
| _version_ | 1811309635935666176 |
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| author | Jueun Kim Jueun Kim Yeong-Jin Choi Honghyun Park Hui-suk Yun Hui-suk Yun |
| author_facet | Jueun Kim Jueun Kim Yeong-Jin Choi Honghyun Park Hui-suk Yun Hui-suk Yun |
| author_sort | Jueun Kim |
| collection | DOAJ |
| description | Novel alginate-hydroxyapatite hybrid microspheres were developed for simultaneous delivery of drugs and cells as a multifunctional bone substitute for osteoporotic bone tissue regeneration. The microspheres were used to enhance osteogenesis and to carry and deliver quercetin, a representative phytoestrogen that controls bone tissue regeneration metabolism in osteoporosis patients, through sustained release over a long period. To overcome quercetin’s hydrophobicity and low solubility in aqueous environments, we added it to the surface of hydroxyapatite (HAp) nanoparticles before mixing them with an alginate solution. The homogeneous distribution of the HAp nanoparticles in the alginate solution was essential for preventing nozzle clogging and achieving successfully fabricated hybrid microspheres. To this end, a 3D ultrasonic treatment was applied. Electrostatic microencapsulation was then used to fabricate hybrid alginate-HAp microspheres containing quercetin and cells. The microspheres were approximately 290.7 ± 42.5 μm (aspect ratio of 1). The sustained release of quercetin was confirmed during a test period of 20 weeks. The cells in the hybrid microspheres maintained good cell viability during the entire testing period, and their osteogenic differentiation behavior was boosted by the presence of HAp. Thus, osteogenic differentiation could be greatly improved by adding quercetin. These novel multi-biofunctional hybrid microspheres have great potential for the regeneration of osteoporotic bone tissue at indeterminate defect sites. |
| first_indexed | 2024-04-13T09:45:02Z |
| format | Article |
| id | doaj.art-b5d494e20a1f4f028cd3c07e8f180b9e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-13T09:45:02Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-b5d494e20a1f4f028cd3c07e8f180b9e2022-12-22T02:51:48ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-08-011010.3389/fbioe.2022.827626827626Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug deliveryJueun Kim0Jueun Kim1Yeong-Jin Choi2Honghyun Park3Hui-suk Yun4Hui-suk Yun5Department of Advanced Materials Engineering, University of Science & Technology (UST), Daejeon, South KoreaCeramic Materials Division, Department of Advanced Biomaterials Research, Korea Institute of Materials Science (KIMS), Changwon, South KoreaCeramic Materials Division, Department of Advanced Biomaterials Research, Korea Institute of Materials Science (KIMS), Changwon, South KoreaCeramic Materials Division, Department of Advanced Biomaterials Research, Korea Institute of Materials Science (KIMS), Changwon, South KoreaDepartment of Advanced Materials Engineering, University of Science & Technology (UST), Daejeon, South KoreaCeramic Materials Division, Department of Advanced Biomaterials Research, Korea Institute of Materials Science (KIMS), Changwon, South KoreaNovel alginate-hydroxyapatite hybrid microspheres were developed for simultaneous delivery of drugs and cells as a multifunctional bone substitute for osteoporotic bone tissue regeneration. The microspheres were used to enhance osteogenesis and to carry and deliver quercetin, a representative phytoestrogen that controls bone tissue regeneration metabolism in osteoporosis patients, through sustained release over a long period. To overcome quercetin’s hydrophobicity and low solubility in aqueous environments, we added it to the surface of hydroxyapatite (HAp) nanoparticles before mixing them with an alginate solution. The homogeneous distribution of the HAp nanoparticles in the alginate solution was essential for preventing nozzle clogging and achieving successfully fabricated hybrid microspheres. To this end, a 3D ultrasonic treatment was applied. Electrostatic microencapsulation was then used to fabricate hybrid alginate-HAp microspheres containing quercetin and cells. The microspheres were approximately 290.7 ± 42.5 μm (aspect ratio of 1). The sustained release of quercetin was confirmed during a test period of 20 weeks. The cells in the hybrid microspheres maintained good cell viability during the entire testing period, and their osteogenic differentiation behavior was boosted by the presence of HAp. Thus, osteogenic differentiation could be greatly improved by adding quercetin. These novel multi-biofunctional hybrid microspheres have great potential for the regeneration of osteoporotic bone tissue at indeterminate defect sites.https://www.frontiersin.org/articles/10.3389/fbioe.2022.827626/fullalginate-hydroxyapatitemicrospherecell and drug deliveryencapsulator3D ultrasonic |
| spellingShingle | Jueun Kim Jueun Kim Yeong-Jin Choi Honghyun Park Hui-suk Yun Hui-suk Yun Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery Frontiers in Bioengineering and Biotechnology alginate-hydroxyapatite microsphere cell and drug delivery encapsulator 3D ultrasonic |
| title | Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery |
| title_full | Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery |
| title_fullStr | Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery |
| title_full_unstemmed | Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery |
| title_short | Fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery |
| title_sort | fabrication of multifunctional alginate microspheres containing hydroxyapatite powder for simultaneous cell and drug delivery |
| topic | alginate-hydroxyapatite microsphere cell and drug delivery encapsulator 3D ultrasonic |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.827626/full |
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