Selected Tetraspanins Functionalized Niosomes as Potential Standards for Exosome Immunoassays
Quantitative detection of exosomes in bio-fluids is a challenging task in a dynamic research field. The absence of a well-established reference material (RM) for method development and inter-comparison studies could be potentially overcome with artificial exosomes: lab-produced biomimetic particles...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/10/5/971 |
| _version_ | 1797567642939162624 |
|---|---|
| author | Pablo García-Manrique Esther Serrano-Pertierra Estefanía Lozano-Andrés Soraya López-Martín María Matos Gemma Gutiérrez María Yáñez-Mó María Carmen Blanco-López |
| author_facet | Pablo García-Manrique Esther Serrano-Pertierra Estefanía Lozano-Andrés Soraya López-Martín María Matos Gemma Gutiérrez María Yáñez-Mó María Carmen Blanco-López |
| author_sort | Pablo García-Manrique |
| collection | DOAJ |
| description | Quantitative detection of exosomes in bio-fluids is a challenging task in a dynamic research field. The absence of a well-established reference material (RM) for method development and inter-comparison studies could be potentially overcome with artificial exosomes: lab-produced biomimetic particles with morphological and functional properties close to natural exosomes. This work presents the design, development and functional characteristics of fully artificial exosomes based on tetraspanin extracellular loops-coated niosomes, produced by bio-nanotechnology methods based on supra-molecular chemistry and recombinant protein technology. Mono- and double-functionalized particles with CD9/CD63 tetraspanins have been developed and characterized from a morphological and functional point of view. Produced bio-particles showed close similarities with natural entities in terms of physical properties. Their utility for bioanalysis is demonstrated by their detection and molecular-type discrimination by enzyme-linked immunosorbent assays (ELISAs), one of the most frequent bio-analytical method found in routine and research labs. The basic material based on streptavidin-coated niosomes allows the surface functionalization with any biotinylated protein or peptide, introducing versatility. Although promising results have been reported, further optimizations and deeper characterization will help this innovative biomaterial become a robust RM for validation and development of diagnostic tools for exosomes determination. |
| first_indexed | 2024-03-10T19:45:05Z |
| format | Article |
| id | doaj.art-b5d593aef92e418281277c1235940212 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-10T19:45:05Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-b5d593aef92e418281277c12359402122023-11-20T00:52:36ZengMDPI AGNanomaterials2079-49912020-05-0110597110.3390/nano10050971Selected Tetraspanins Functionalized Niosomes as Potential Standards for Exosome ImmunoassaysPablo García-Manrique0Esther Serrano-Pertierra1Estefanía Lozano-Andrés2Soraya López-Martín3María Matos4Gemma Gutiérrez5María Yáñez-Mó6María Carmen Blanco-López7Department of Physical and Analytical Chemistry, University of Oviedo, 33006 Asturias, SpainDepartment of Physical and Analytical Chemistry, University of Oviedo, 33006 Asturias, SpainDepartamento de Biología Molecular, Universidad Autónoma de Madrid (UAM), 28049 Madrid, SpainDepartamento de Biología Molecular, Universidad Autónoma de Madrid (UAM), 28049 Madrid, SpainDepartment of Chemical Engineering and Environmental Technology, University of Oviedo, 33006 Asturias, SpainDepartment of Chemical Engineering and Environmental Technology, University of Oviedo, 33006 Asturias, SpainDepartamento de Biología Molecular, Universidad Autónoma de Madrid (UAM), 28049 Madrid, SpainDepartment of Physical and Analytical Chemistry, University of Oviedo, 33006 Asturias, SpainQuantitative detection of exosomes in bio-fluids is a challenging task in a dynamic research field. The absence of a well-established reference material (RM) for method development and inter-comparison studies could be potentially overcome with artificial exosomes: lab-produced biomimetic particles with morphological and functional properties close to natural exosomes. This work presents the design, development and functional characteristics of fully artificial exosomes based on tetraspanin extracellular loops-coated niosomes, produced by bio-nanotechnology methods based on supra-molecular chemistry and recombinant protein technology. Mono- and double-functionalized particles with CD9/CD63 tetraspanins have been developed and characterized from a morphological and functional point of view. Produced bio-particles showed close similarities with natural entities in terms of physical properties. Their utility for bioanalysis is demonstrated by their detection and molecular-type discrimination by enzyme-linked immunosorbent assays (ELISAs), one of the most frequent bio-analytical method found in routine and research labs. The basic material based on streptavidin-coated niosomes allows the surface functionalization with any biotinylated protein or peptide, introducing versatility. Although promising results have been reported, further optimizations and deeper characterization will help this innovative biomaterial become a robust RM for validation and development of diagnostic tools for exosomes determination.https://www.mdpi.com/2079-4991/10/5/971Artificial Extracellular Vesicles (EVs)tetraspaninsanalytical standardsimmunoassays developmentbiomimetic materials |
| spellingShingle | Pablo García-Manrique Esther Serrano-Pertierra Estefanía Lozano-Andrés Soraya López-Martín María Matos Gemma Gutiérrez María Yáñez-Mó María Carmen Blanco-López Selected Tetraspanins Functionalized Niosomes as Potential Standards for Exosome Immunoassays Nanomaterials Artificial Extracellular Vesicles (EVs) tetraspanins analytical standards immunoassays development biomimetic materials |
| title | Selected Tetraspanins Functionalized Niosomes as Potential Standards for Exosome Immunoassays |
| title_full | Selected Tetraspanins Functionalized Niosomes as Potential Standards for Exosome Immunoassays |
| title_fullStr | Selected Tetraspanins Functionalized Niosomes as Potential Standards for Exosome Immunoassays |
| title_full_unstemmed | Selected Tetraspanins Functionalized Niosomes as Potential Standards for Exosome Immunoassays |
| title_short | Selected Tetraspanins Functionalized Niosomes as Potential Standards for Exosome Immunoassays |
| title_sort | selected tetraspanins functionalized niosomes as potential standards for exosome immunoassays |
| topic | Artificial Extracellular Vesicles (EVs) tetraspanins analytical standards immunoassays development biomimetic materials |
| url | https://www.mdpi.com/2079-4991/10/5/971 |
| work_keys_str_mv | AT pablogarciamanrique selectedtetraspaninsfunctionalizedniosomesaspotentialstandardsforexosomeimmunoassays AT estherserranopertierra selectedtetraspaninsfunctionalizedniosomesaspotentialstandardsforexosomeimmunoassays AT estefanialozanoandres selectedtetraspaninsfunctionalizedniosomesaspotentialstandardsforexosomeimmunoassays AT sorayalopezmartin selectedtetraspaninsfunctionalizedniosomesaspotentialstandardsforexosomeimmunoassays AT mariamatos selectedtetraspaninsfunctionalizedniosomesaspotentialstandardsforexosomeimmunoassays AT gemmagutierrez selectedtetraspaninsfunctionalizedniosomesaspotentialstandardsforexosomeimmunoassays AT mariayanezmo selectedtetraspaninsfunctionalizedniosomesaspotentialstandardsforexosomeimmunoassays AT mariacarmenblancolopez selectedtetraspaninsfunctionalizedniosomesaspotentialstandardsforexosomeimmunoassays |