Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart
Aim: Sphingosine 1-phosphate (S1P), sphingolipid derivatives are known anti-inflammatory, anti-apoptotic, and anti-oxidant agent. S1P have been demonstrated to have a role in the cardiovascular system. The purpose of this study was to understand the precise expression and distribution of S1P recepto...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphar.2017.00312/full |
| _version_ | 1818650023220477952 |
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| author | Naseer Ahmed Naseer Ahmed Naseer Ahmed Daniele Linardi Ilaria Decimo Riffat Mehboob Mebratu A. Gebrie Giulio Innamorati Giovanni B. Luciani Giuseppe Faggian Alessio Rungatscher |
| author_facet | Naseer Ahmed Naseer Ahmed Naseer Ahmed Daniele Linardi Ilaria Decimo Riffat Mehboob Mebratu A. Gebrie Giulio Innamorati Giovanni B. Luciani Giuseppe Faggian Alessio Rungatscher |
| author_sort | Naseer Ahmed |
| collection | DOAJ |
| description | Aim: Sphingosine 1-phosphate (S1P), sphingolipid derivatives are known anti-inflammatory, anti-apoptotic, and anti-oxidant agent. S1P have been demonstrated to have a role in the cardiovascular system. The purpose of this study was to understand the precise expression and distribution of S1P receptors (S1PRs) in human and rat cardiovascular tissues to know the significance and possible implementation of our experimental studies in rat models.Methods and Results: In this study, we investigated the localization of S1PRs in human heart samples from cardiac surgery department, University of Verona Hospital and rat samples. Immunohistochemical investigation of paraffin-embedded sections illustrated diffused staining of the myocardial samples from human and rat. The signals of the human heart were similar to those of the rat heart in all chambers of the heart. The immunohistochemical expression levels correlated well with the results of RT-PCR-based analysis and western blotting. We confirmed by all techniques that S1PR1 expressed strongly as compared to S1PR3, and are uniformly distributed in all chambers of the heart with no significant difference in human and rat myocardial tissue. S1PR2 expression was significantly weak while S1PR4 and S1PR5 were not detectable in RT-PCR results in both human and rat heart.Conclusion: These results indicate that experimental studies using S1PR agonists on rat models are more likely to have a potential for translation into clinical studies, and second important information revealed by this study is, S1P receptor agonist can be used for cardioprotection in global ischemia-reperfusion injury. |
| first_indexed | 2024-12-17T01:43:37Z |
| format | Article |
| id | doaj.art-b5d61a0731f34b2680a9349c7e232512 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-17T01:43:37Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-b5d61a0731f34b2680a9349c7e2325122022-12-21T22:08:16ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122017-05-01810.3389/fphar.2017.00312267361Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat HeartNaseer Ahmed0Naseer Ahmed1Naseer Ahmed2Daniele Linardi3Ilaria Decimo4Riffat Mehboob5Mebratu A. Gebrie6Giulio Innamorati7Giovanni B. Luciani8Giuseppe Faggian9Alessio Rungatscher10Cardiac Surgery Division, University of Verona Medical SchoolVerona, ItalyTranslational Surgery Lab, University of Verona Medical SchoolVerona, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of VeronaVerona, ItalyCardiac Surgery Division, University of Verona Medical SchoolVerona, ItalySection of Pharmacology, Department of Diagnostics and Public Health, University of VeronaVerona, ItalyDepartment of Biomedical Sciences, King Edward Medical UniversityLahore, PakistanCardiac Surgery Division, University of Verona Medical SchoolVerona, ItalyTranslational Surgery Lab, University of Verona Medical SchoolVerona, ItalyCardiac Surgery Division, University of Verona Medical SchoolVerona, ItalyCardiac Surgery Division, University of Verona Medical SchoolVerona, ItalyCardiac Surgery Division, University of Verona Medical SchoolVerona, ItalyAim: Sphingosine 1-phosphate (S1P), sphingolipid derivatives are known anti-inflammatory, anti-apoptotic, and anti-oxidant agent. S1P have been demonstrated to have a role in the cardiovascular system. The purpose of this study was to understand the precise expression and distribution of S1P receptors (S1PRs) in human and rat cardiovascular tissues to know the significance and possible implementation of our experimental studies in rat models.Methods and Results: In this study, we investigated the localization of S1PRs in human heart samples from cardiac surgery department, University of Verona Hospital and rat samples. Immunohistochemical investigation of paraffin-embedded sections illustrated diffused staining of the myocardial samples from human and rat. The signals of the human heart were similar to those of the rat heart in all chambers of the heart. The immunohistochemical expression levels correlated well with the results of RT-PCR-based analysis and western blotting. We confirmed by all techniques that S1PR1 expressed strongly as compared to S1PR3, and are uniformly distributed in all chambers of the heart with no significant difference in human and rat myocardial tissue. S1PR2 expression was significantly weak while S1PR4 and S1PR5 were not detectable in RT-PCR results in both human and rat heart.Conclusion: These results indicate that experimental studies using S1PR agonists on rat models are more likely to have a potential for translation into clinical studies, and second important information revealed by this study is, S1P receptor agonist can be used for cardioprotection in global ischemia-reperfusion injury.http://journal.frontiersin.org/article/10.3389/fphar.2017.00312/fullsphingosine 1-phosphate receptorshearthumanratcardioprotectionischemia-reperfusion injury |
| spellingShingle | Naseer Ahmed Naseer Ahmed Naseer Ahmed Daniele Linardi Ilaria Decimo Riffat Mehboob Mebratu A. Gebrie Giulio Innamorati Giovanni B. Luciani Giuseppe Faggian Alessio Rungatscher Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart Frontiers in Pharmacology sphingosine 1-phosphate receptors heart human rat cardioprotection ischemia-reperfusion injury |
| title | Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart |
| title_full | Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart |
| title_fullStr | Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart |
| title_full_unstemmed | Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart |
| title_short | Characterization and Expression of Sphingosine 1-Phosphate Receptors in Human and Rat Heart |
| title_sort | characterization and expression of sphingosine 1 phosphate receptors in human and rat heart |
| topic | sphingosine 1-phosphate receptors heart human rat cardioprotection ischemia-reperfusion injury |
| url | http://journal.frontiersin.org/article/10.3389/fphar.2017.00312/full |
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