Marker Substances in the Aroma of Truffles

The aim of this study was to identify specific truffle marker substances within the truffle aroma. The aroma profile of different truffle species was analyzed using static headspace sampling with gas chromatography mass spectrometry analysis (SHS/GC-MS). Possible marker substances were identified, t...

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Main Authors: Ruben Epping, Lilly Bliesener, Tilman Weiss, Matthias Koch
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/27/16/5169
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author Ruben Epping
Lilly Bliesener
Tilman Weiss
Matthias Koch
author_facet Ruben Epping
Lilly Bliesener
Tilman Weiss
Matthias Koch
author_sort Ruben Epping
collection DOAJ
description The aim of this study was to identify specific truffle marker substances within the truffle aroma. The aroma profile of different truffle species was analyzed using static headspace sampling with gas chromatography mass spectrometry analysis (SHS/GC-MS). Possible marker substances were identified, taking the additional literature into account. The selected marker substances were tested in an experiment with 19 truffle dogs. The hypothesis “If trained truffle dogs recognize the substances as supposed truffles in the context of an experiment, they can be regarded as specific” was made. As it would be nearly impossible to investigate every other possible emitter of the same compounds to determine their specificity, this hypothesis was a reasonable approximation. We were interested in the question of what it is the dogs actually search for on a chemical level and whether we can link their ability to find truffles to one or more specific marker substances. The results of the dog experiment are not as unambiguous as could have been expected based on the SHS/GC-MS measurements. Presumably, the truffle aroma is mainly characterized and perceived by dogs by dimethyl sulfide and dimethyl disulfide. However, as dogs are living beings and not analytical instruments, it seems unavoidable that one must live with some degree of uncertainty regarding these results.
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spelling doaj.art-b5d61f5a1a4e4aef9ebb531484840cb62023-12-02T00:04:23ZengMDPI AGMolecules1420-30492022-08-012716516910.3390/molecules27165169Marker Substances in the Aroma of TrufflesRuben Epping0Lilly Bliesener1Tilman Weiss2Matthias Koch3Division of Organic Trace Analysis and Food Analysis, Bundesanstalt für Materialforschung und -Prüfung, 12489 Berlin, GermanyDivision of Organic Trace Analysis and Food Analysis, Bundesanstalt für Materialforschung und -Prüfung, 12489 Berlin, GermanySglux SolGel Technologies GmbH, 12489 Berlin, GermanyDivision of Organic Trace Analysis and Food Analysis, Bundesanstalt für Materialforschung und -Prüfung, 12489 Berlin, GermanyThe aim of this study was to identify specific truffle marker substances within the truffle aroma. The aroma profile of different truffle species was analyzed using static headspace sampling with gas chromatography mass spectrometry analysis (SHS/GC-MS). Possible marker substances were identified, taking the additional literature into account. The selected marker substances were tested in an experiment with 19 truffle dogs. The hypothesis “If trained truffle dogs recognize the substances as supposed truffles in the context of an experiment, they can be regarded as specific” was made. As it would be nearly impossible to investigate every other possible emitter of the same compounds to determine their specificity, this hypothesis was a reasonable approximation. We were interested in the question of what it is the dogs actually search for on a chemical level and whether we can link their ability to find truffles to one or more specific marker substances. The results of the dog experiment are not as unambiguous as could have been expected based on the SHS/GC-MS measurements. Presumably, the truffle aroma is mainly characterized and perceived by dogs by dimethyl sulfide and dimethyl disulfide. However, as dogs are living beings and not analytical instruments, it seems unavoidable that one must live with some degree of uncertainty regarding these results.https://www.mdpi.com/1420-3049/27/16/5169trufflevolatile organic compoundsgas chromatographymass spectrometrycanine olfactometry
spellingShingle Ruben Epping
Lilly Bliesener
Tilman Weiss
Matthias Koch
Marker Substances in the Aroma of Truffles
Molecules
truffle
volatile organic compounds
gas chromatography
mass spectrometry
canine olfactometry
title Marker Substances in the Aroma of Truffles
title_full Marker Substances in the Aroma of Truffles
title_fullStr Marker Substances in the Aroma of Truffles
title_full_unstemmed Marker Substances in the Aroma of Truffles
title_short Marker Substances in the Aroma of Truffles
title_sort marker substances in the aroma of truffles
topic truffle
volatile organic compounds
gas chromatography
mass spectrometry
canine olfactometry
url https://www.mdpi.com/1420-3049/27/16/5169
work_keys_str_mv AT rubenepping markersubstancesinthearomaoftruffles
AT lillybliesener markersubstancesinthearomaoftruffles
AT tilmanweiss markersubstancesinthearomaoftruffles
AT matthiaskoch markersubstancesinthearomaoftruffles