Glycoalkaloid Composition and Flavonoid Content as Driving Forces of Phytotoxicity in Diploid Potato
Despite their advantages, biotechnological and omic techniques have not been applied often to characterize phytotoxicity in depth. Here, we show the distribution of phytotoxicity and glycoalkaloid content in a diploid potato population and try to clarify the source of variability of phytotoxicity am...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/2/1657 |
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| author | Katarzyna Szajko Paulina Smyda-Dajmund Jarosław Ciekot Waldemar Marczewski Dorota Sołtys-Kalina |
| author_facet | Katarzyna Szajko Paulina Smyda-Dajmund Jarosław Ciekot Waldemar Marczewski Dorota Sołtys-Kalina |
| author_sort | Katarzyna Szajko |
| collection | DOAJ |
| description | Despite their advantages, biotechnological and omic techniques have not been applied often to characterize phytotoxicity in depth. Here, we show the distribution of phytotoxicity and glycoalkaloid content in a diploid potato population and try to clarify the source of variability of phytotoxicity among plants whose leaf extracts have a high glycoalkaloid content against the test plant species, mustard. Six glycoalkaloids were recognized in the potato leaf extracts: solasonine, solamargine, α-solanine, α-chaconine, leptinine I, and leptine II. The glycoalkaloid profiles of the progeny of the group with high phytotoxicity differed from those of the progeny of the group with low phytotoxicity, which stimulated mustard growth. RNA sequencing analysis revealed that the upregulated <i>flavonol synthase/flavonone 3-hydroxylase-like</i> gene was expressed in the progeny of the low phytotoxicity group, stimulating plant growth. We concluded that the metabolic shift among potato progeny may be a source of different physiological responses in mustard. The composition of glycoalkaloids, rather than the total glycoalkaloid content itself, in potato leaf extracts, may be a driving force of phytotoxicity. We suggest that, in addition to glycoalkaloids, other metabolites may shape phytotoxicity, and we assume that these metabolites may be flavonoids. |
| first_indexed | 2024-03-09T12:19:09Z |
| format | Article |
| id | doaj.art-b5da0d7886d34b7fb927e9f56af972a7 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:19:09Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-b5da0d7886d34b7fb927e9f56af972a72023-11-30T22:43:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242165710.3390/ijms24021657Glycoalkaloid Composition and Flavonoid Content as Driving Forces of Phytotoxicity in Diploid PotatoKatarzyna Szajko0Paulina Smyda-Dajmund1Jarosław Ciekot2Waldemar Marczewski3Dorota Sołtys-Kalina4Plant Breeding and Acclimatization Institute–National Research Institute, Platanowa 19, 05-831 Młochów, PolandPlant Breeding and Acclimatization Institute–National Research Institute, Platanowa 19, 05-831 Młochów, PolandLaboratory of Biomedical Chemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Rudolfa Weigla 12, 53-114 Wrocław, PolandPlant Breeding and Acclimatization Institute–National Research Institute, Platanowa 19, 05-831 Młochów, PolandPlant Breeding and Acclimatization Institute–National Research Institute, Platanowa 19, 05-831 Młochów, PolandDespite their advantages, biotechnological and omic techniques have not been applied often to characterize phytotoxicity in depth. Here, we show the distribution of phytotoxicity and glycoalkaloid content in a diploid potato population and try to clarify the source of variability of phytotoxicity among plants whose leaf extracts have a high glycoalkaloid content against the test plant species, mustard. Six glycoalkaloids were recognized in the potato leaf extracts: solasonine, solamargine, α-solanine, α-chaconine, leptinine I, and leptine II. The glycoalkaloid profiles of the progeny of the group with high phytotoxicity differed from those of the progeny of the group with low phytotoxicity, which stimulated mustard growth. RNA sequencing analysis revealed that the upregulated <i>flavonol synthase/flavonone 3-hydroxylase-like</i> gene was expressed in the progeny of the low phytotoxicity group, stimulating plant growth. We concluded that the metabolic shift among potato progeny may be a source of different physiological responses in mustard. The composition of glycoalkaloids, rather than the total glycoalkaloid content itself, in potato leaf extracts, may be a driving force of phytotoxicity. We suggest that, in addition to glycoalkaloids, other metabolites may shape phytotoxicity, and we assume that these metabolites may be flavonoids.https://www.mdpi.com/1422-0067/24/2/1657allelopathygene expressiongene ontologyleptinesolamarginesolasonine |
| spellingShingle | Katarzyna Szajko Paulina Smyda-Dajmund Jarosław Ciekot Waldemar Marczewski Dorota Sołtys-Kalina Glycoalkaloid Composition and Flavonoid Content as Driving Forces of Phytotoxicity in Diploid Potato International Journal of Molecular Sciences allelopathy gene expression gene ontology leptine solamargine solasonine |
| title | Glycoalkaloid Composition and Flavonoid Content as Driving Forces of Phytotoxicity in Diploid Potato |
| title_full | Glycoalkaloid Composition and Flavonoid Content as Driving Forces of Phytotoxicity in Diploid Potato |
| title_fullStr | Glycoalkaloid Composition and Flavonoid Content as Driving Forces of Phytotoxicity in Diploid Potato |
| title_full_unstemmed | Glycoalkaloid Composition and Flavonoid Content as Driving Forces of Phytotoxicity in Diploid Potato |
| title_short | Glycoalkaloid Composition and Flavonoid Content as Driving Forces of Phytotoxicity in Diploid Potato |
| title_sort | glycoalkaloid composition and flavonoid content as driving forces of phytotoxicity in diploid potato |
| topic | allelopathy gene expression gene ontology leptine solamargine solasonine |
| url | https://www.mdpi.com/1422-0067/24/2/1657 |
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