Relevance of Additional Immunohistochemical Markers in the Differential Diagnosis of Small B-Cell Lymphomas: A Case-Control Study
Objective: Clinical and pathological differential diagnosis of small B-cell lymphomas (SBCLs) is still controversial and may be difficult due to their overlapping morphology, phenotype, and differentiation to plasma cells. We aimed to examine the expression of the immune receptor translocation-assoc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2022-08-01
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| Series: | Turkish Journal of Hematology |
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| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-05998 |
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| author | Hale Kıvrak Seher Yüksel Can Ateş Mustafa Merter Gülşah Kaygusuz Muhit Özcan Işınsu Kuzu |
| author_facet | Hale Kıvrak Seher Yüksel Can Ateş Mustafa Merter Gülşah Kaygusuz Muhit Özcan Işınsu Kuzu |
| author_sort | Hale Kıvrak |
| collection | DOAJ |
| description | Objective: Clinical and pathological differential diagnosis of small B-cell lymphomas (SBCLs) is still controversial and may be difficult due to their overlapping morphology, phenotype, and differentiation to plasma cells. We aimed to examine the expression of the immune receptor translocation-associated protein 1 (IRTA1), myeloid cell nuclear differentiation antigen (MNDA), lymphoid enhancer-binding factor-1 (LEF1), and stathmin 1 (STMN1) markers in SBCL cases involving different sites that may have plasma cell differentiation. Materials and Methods: We studied 154 tissue samples with lymphoma involvement from 116 patients and evaluated the staining distribution of the markers. Expressions were evaluated in 21 chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), 7 follicular lymphoma (FL), 14 nodal marginal zone lymphoma, 17 extranodal marginal zone lymphoma, 55 splenic marginal zone lymphoma, 22 marginal zone lymphoma-not otherwise specified, and 18 lymphoplasmacytic lymphoma/Waldenström macroglobulinemia cases by immunohistochemistry. Results: The results confirmed that LEF1 was the most sensitive and specific marker for CLL/SLL and STMN1 was the most sensitive and specific marker for FL (p<0.001). MNDA and IRTA1 were useful markers to distinguish marginal zone lymphomas. Conclusion: Our results suggest that LEF1 for CLL/SLL and STMN1 for FL are reliable markers. LEF1, MNDA, STMN1, and IRTA1 are helpful with other routinely used immunohistochemical markers in a diagnostic algorithm considering their limitations. |
| first_indexed | 2024-04-10T13:05:11Z |
| format | Article |
| id | doaj.art-b5dfec53013b43799cafb5fe4b3ca34b |
| institution | Directory Open Access Journal |
| issn | 1308-5263 |
| language | English |
| last_indexed | 2024-04-10T13:05:11Z |
| publishDate | 2022-08-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Turkish Journal of Hematology |
| spelling | doaj.art-b5dfec53013b43799cafb5fe4b3ca34b2023-02-15T16:12:58ZengGalenos Publishing HouseTurkish Journal of Hematology1308-52632022-08-0139317818710.4274/tjh.galenos.2021.2021.0349TJH-05998Relevance of Additional Immunohistochemical Markers in the Differential Diagnosis of Small B-Cell Lymphomas: A Case-Control StudyHale Kıvrak0Seher Yüksel1Can Ateş2Mustafa Merter3Gülşah Kaygusuz4Muhit Özcan5Işınsu Kuzu6Ankara University Faculty of Medicine, Department of Pathology, Ankara, TurkeyAnkara University Faculty of Medicine, Department of Pathology, Ankara, TurkeyAksaray University Faculty of Medicine, Department of Biostatistics and Medical Informatics, Aksaray, Turkey; Ankara University Faculty of Medicine, Department of Biostatistics, Ankara, TurkeyFırat University Faculty of Medicine, Department of Hematology, Elazığ, Turkey; Ankara University Faculty of Medicine, Department of Hematology, Ankara, TurkeyAnkara University Faculty of Medicine, Department of Pathology, Ankara, TurkeyAnkara University Faculty of Medicine, Department of Hematology, Ankara, TurkeyAnkara University Faculty of Medicine, Department of Pathology, Ankara, TurkeyObjective: Clinical and pathological differential diagnosis of small B-cell lymphomas (SBCLs) is still controversial and may be difficult due to their overlapping morphology, phenotype, and differentiation to plasma cells. We aimed to examine the expression of the immune receptor translocation-associated protein 1 (IRTA1), myeloid cell nuclear differentiation antigen (MNDA), lymphoid enhancer-binding factor-1 (LEF1), and stathmin 1 (STMN1) markers in SBCL cases involving different sites that may have plasma cell differentiation. Materials and Methods: We studied 154 tissue samples with lymphoma involvement from 116 patients and evaluated the staining distribution of the markers. Expressions were evaluated in 21 chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), 7 follicular lymphoma (FL), 14 nodal marginal zone lymphoma, 17 extranodal marginal zone lymphoma, 55 splenic marginal zone lymphoma, 22 marginal zone lymphoma-not otherwise specified, and 18 lymphoplasmacytic lymphoma/Waldenström macroglobulinemia cases by immunohistochemistry. Results: The results confirmed that LEF1 was the most sensitive and specific marker for CLL/SLL and STMN1 was the most sensitive and specific marker for FL (p<0.001). MNDA and IRTA1 were useful markers to distinguish marginal zone lymphomas. Conclusion: Our results suggest that LEF1 for CLL/SLL and STMN1 for FL are reliable markers. LEF1, MNDA, STMN1, and IRTA1 are helpful with other routinely used immunohistochemical markers in a diagnostic algorithm considering their limitations.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-05998small b-cell lymphomamndalef1stathmin 1irta1 |
| spellingShingle | Hale Kıvrak Seher Yüksel Can Ateş Mustafa Merter Gülşah Kaygusuz Muhit Özcan Işınsu Kuzu Relevance of Additional Immunohistochemical Markers in the Differential Diagnosis of Small B-Cell Lymphomas: A Case-Control Study Turkish Journal of Hematology small b-cell lymphoma mnda lef1 stathmin 1 irta1 |
| title | Relevance of Additional Immunohistochemical Markers in the Differential Diagnosis of Small B-Cell Lymphomas: A Case-Control Study |
| title_full | Relevance of Additional Immunohistochemical Markers in the Differential Diagnosis of Small B-Cell Lymphomas: A Case-Control Study |
| title_fullStr | Relevance of Additional Immunohistochemical Markers in the Differential Diagnosis of Small B-Cell Lymphomas: A Case-Control Study |
| title_full_unstemmed | Relevance of Additional Immunohistochemical Markers in the Differential Diagnosis of Small B-Cell Lymphomas: A Case-Control Study |
| title_short | Relevance of Additional Immunohistochemical Markers in the Differential Diagnosis of Small B-Cell Lymphomas: A Case-Control Study |
| title_sort | relevance of additional immunohistochemical markers in the differential diagnosis of small b cell lymphomas a case control study |
| topic | small b-cell lymphoma mnda lef1 stathmin 1 irta1 |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=tjh&un=TJH-05998 |
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