<em>Globularia alypum</em> L. and Related Species: LC-MS Profiles and Antidiabetic, Antioxidant, Anti-Inflammatory, Antibacterial and Anticancer Potential
Species from the genus <i>Globularia</i> L. have been used as healing agents for various ailments, with utilization of <i>Globularia alypum</i> L. being most frequently reported. The aim of this study was to evaluate the antidiabetic, antioxidant, anti-inflammatory, antibacte...
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2022-04-01
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| author | Maja Friščić Roberta Petlevski Ivan Kosalec Josip Madunić Maja Matulić Franz Bucar Kroata Hazler Pilepić Željan Maleš |
| author_facet | Maja Friščić Roberta Petlevski Ivan Kosalec Josip Madunić Maja Matulić Franz Bucar Kroata Hazler Pilepić Željan Maleš |
| author_sort | Maja Friščić |
| collection | DOAJ |
| description | Species from the genus <i>Globularia</i> L. have been used as healing agents for various ailments, with utilization of <i>Globularia alypum</i> L. being most frequently reported. The aim of this study was to evaluate the antidiabetic, antioxidant, anti-inflammatory, antibacterial and anticancer potential of <i>G. alypum</i> and three related species, <i>G. punctata</i> Lapeyr., <i>G. cordifolia</i> L. and <i>G. meridionalis</i> (Podp.) O.Schwarz, in relation to their phytochemical compositions. Globularin and verbascoside were identified using LC-PDA-ESI-MS<sup>n</sup> as the major metabolites of <i>G. alypum</i> with known biological activities. <i>G. alypum</i> demonstrated the greatest α-glucosidase inhibitory activity and DPPH radical scavenging activity (IC<sub>50</sub> = 17.25 μg/mL), while its anti-inflammatory activity was not significantly different from those of related species. All investigated species showed considerable antibacterial activity against methicillin-resistant <i>Staphylococcus aureus</i> in the broth microdilution method (MIC = 1.42–3.79 mg/mL). <i>G. punctata</i> also showed antibacterial activities against <i>Escherichia coli</i> (MIC = 1.42 mg/mL), <i>Bacillus subtilis</i> (MIC = 1.89 mg/mL), <i>B. cereus</i> (MIC = 2.84 mg/mL) and <i>Enterococcus faecalis</i> (MBC = 5.68 mg/mL). <i>G. punctata</i>, <i>G. cordifolia</i> and <i>G. meridionalis</i> showed greater anticancer potential than <i>G. alypum</i>. Obtained results indicate investigated <i>Globularia</i> species could serve as sources of diverse bioactive molecules, with <i>G. punctata</i> having the greatest antibacterial potential. |
| first_indexed | 2024-03-10T03:10:44Z |
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| id | doaj.art-b5e0bb89ef4a44e08e777c195d12dd12 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T03:10:44Z |
| publishDate | 2022-04-01 |
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| spelling | doaj.art-b5e0bb89ef4a44e08e777c195d12dd122023-11-23T12:33:36ZengMDPI AGPharmaceuticals1424-82472022-04-0115550610.3390/ph15050506<em>Globularia alypum</em> L. and Related Species: LC-MS Profiles and Antidiabetic, Antioxidant, Anti-Inflammatory, Antibacterial and Anticancer PotentialMaja Friščić0Roberta Petlevski1Ivan Kosalec2Josip Madunić3Maja Matulić4Franz Bucar5Kroata Hazler Pilepić6Željan Maleš7Department of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry, University of Zagreb, Schrottova 39, HR-10000 Zagreb, CroatiaDepartment of Medical Biochemistry and Hematology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Domagojeva 2, HR-10000 Zagreb, CroatiaDepartment of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Schrottova 39, HR-10000 Zagreb, CroatiaBiochemistry and Organic Analytical Chemistry Unit, Institute for Medical Research and Occupational Health, Ksaverska cesta 2, HR-10001 Zagreb, CroatiaDepartment of Biology, Division of Molecular Biology, Faculty of Science, University of Zagreb, Horvatovac 102A, HR-10000 Zagreb, CroatiaDepartment of Pharmacognosy, Institute of Pharmaceutical Sciences, University of Graz, Beethovenstraße 8, A-8010 Graz, AustriaDepartment of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry, University of Zagreb, Schrottova 39, HR-10000 Zagreb, CroatiaDepartment of Pharmaceutical Botany, Faculty of Pharmacy and Biochemistry, University of Zagreb, Schrottova 39, HR-10000 Zagreb, CroatiaSpecies from the genus <i>Globularia</i> L. have been used as healing agents for various ailments, with utilization of <i>Globularia alypum</i> L. being most frequently reported. The aim of this study was to evaluate the antidiabetic, antioxidant, anti-inflammatory, antibacterial and anticancer potential of <i>G. alypum</i> and three related species, <i>G. punctata</i> Lapeyr., <i>G. cordifolia</i> L. and <i>G. meridionalis</i> (Podp.) O.Schwarz, in relation to their phytochemical compositions. Globularin and verbascoside were identified using LC-PDA-ESI-MS<sup>n</sup> as the major metabolites of <i>G. alypum</i> with known biological activities. <i>G. alypum</i> demonstrated the greatest α-glucosidase inhibitory activity and DPPH radical scavenging activity (IC<sub>50</sub> = 17.25 μg/mL), while its anti-inflammatory activity was not significantly different from those of related species. All investigated species showed considerable antibacterial activity against methicillin-resistant <i>Staphylococcus aureus</i> in the broth microdilution method (MIC = 1.42–3.79 mg/mL). <i>G. punctata</i> also showed antibacterial activities against <i>Escherichia coli</i> (MIC = 1.42 mg/mL), <i>Bacillus subtilis</i> (MIC = 1.89 mg/mL), <i>B. cereus</i> (MIC = 2.84 mg/mL) and <i>Enterococcus faecalis</i> (MBC = 5.68 mg/mL). <i>G. punctata</i>, <i>G. cordifolia</i> and <i>G. meridionalis</i> showed greater anticancer potential than <i>G. alypum</i>. Obtained results indicate investigated <i>Globularia</i> species could serve as sources of diverse bioactive molecules, with <i>G. punctata</i> having the greatest antibacterial potential.https://www.mdpi.com/1424-8247/15/5/506<i>Globularia alypum</i>metabolite profilinghyperglycemia-induced oxidative stressantiradical activitycyclooxygenase-1well diffusion |
| spellingShingle | Maja Friščić Roberta Petlevski Ivan Kosalec Josip Madunić Maja Matulić Franz Bucar Kroata Hazler Pilepić Željan Maleš <em>Globularia alypum</em> L. and Related Species: LC-MS Profiles and Antidiabetic, Antioxidant, Anti-Inflammatory, Antibacterial and Anticancer Potential Pharmaceuticals <i>Globularia alypum</i> metabolite profiling hyperglycemia-induced oxidative stress antiradical activity cyclooxygenase-1 well diffusion |
| title | <em>Globularia alypum</em> L. and Related Species: LC-MS Profiles and Antidiabetic, Antioxidant, Anti-Inflammatory, Antibacterial and Anticancer Potential |
| title_full | <em>Globularia alypum</em> L. and Related Species: LC-MS Profiles and Antidiabetic, Antioxidant, Anti-Inflammatory, Antibacterial and Anticancer Potential |
| title_fullStr | <em>Globularia alypum</em> L. and Related Species: LC-MS Profiles and Antidiabetic, Antioxidant, Anti-Inflammatory, Antibacterial and Anticancer Potential |
| title_full_unstemmed | <em>Globularia alypum</em> L. and Related Species: LC-MS Profiles and Antidiabetic, Antioxidant, Anti-Inflammatory, Antibacterial and Anticancer Potential |
| title_short | <em>Globularia alypum</em> L. and Related Species: LC-MS Profiles and Antidiabetic, Antioxidant, Anti-Inflammatory, Antibacterial and Anticancer Potential |
| title_sort | em globularia alypum em l and related species lc ms profiles and antidiabetic antioxidant anti inflammatory antibacterial and anticancer potential |
| topic | <i>Globularia alypum</i> metabolite profiling hyperglycemia-induced oxidative stress antiradical activity cyclooxygenase-1 well diffusion |
| url | https://www.mdpi.com/1424-8247/15/5/506 |
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