Modulation of Negative Effects of Physiological Stress on Frozen-Thawed Semen with Nutrition of Organic Selenium in Ross 308 Rooster
Current experiment was carried out in factorial 2×2 arrangement to study the effects of stress (with or without dexamethasone administration) and addition of dietary selenium (with or without selenium supplementation in the diet) in male broiler breeder on the quality of frozen-thawed sperm under ox...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Razi Vaccine and Serum Research Institute
2021-12-01
|
| Series: | Archives of Razi Institute |
| Subjects: | |
| Online Access: | https://archrazi.areeo.ac.ir/article_125582_ac2a1adae71a683955d9326e7d219020.pdf |
| _version_ | 1811318818210840576 |
|---|---|
| author | N Kamrani A Karimi M Nazari R Masoudi |
| author_facet | N Kamrani A Karimi M Nazari R Masoudi |
| author_sort | N Kamrani |
| collection | DOAJ |
| description | Current experiment was carried out in factorial 2×2 arrangement to study the effects of stress (with or without dexamethasone administration) and addition of dietary selenium (with or without selenium supplementation in the diet) in male broiler breeder on the quality of frozen-thawed sperm under oxidative stress induced by dexamethasone. A total of 24 broiler breeder roosters with the age of 28 weeks were used based on a completely randomized design with four therapeutic approaches (factorial 2×2) and six birds in each approach. The experimental treatments were: 1) basal diet without selenium supplementation and injection of saline (CON), 2) basal diet with dexamethasone injection (4 mg/kg BW, three times every other day for one week), (DEX), 3) without dexamethasone injection and supplementation with 0.3 mg/kg selenium (Sel-Plex), and 4) dexamethasone injection and basal diet supplemented with 0.3 mg/kg of diet selenium (Sel-Plex+Dex). Sperm samples were collected from roosters. Motility, progressive motility, plasma membrane integrity, viability, malondialdehyde concentration and antioxidant parameters were evaluated in fresh and frozen-thawed semen. In spite of non-significant interaction effects, factorial analysis indicated the significant effect of every factor on different experimental parameters in fresh and frozen-thawed semen (P<0.05); The results revealed that total and progressive motility, plasma membrane integrity and viability were lower in DEX group when compared with other treatments (P<0.05). On the other hand, malondialdehyde concentration was higher in DEX group in comparison with Con, Sel-Plex and Sel-Plex+DEX groups (P<0.05). Moreover, total antioxidant capacity, level of glutathione peroxidase and superoxide dismutase were lower in DEX group as compared with other treatments (P<0.05). Our findings indicated that administration of selenium in dexamethasone-receiving roosters (Sel-Plex+DEX) improved the parameters of fresh and frozen-thawed sperm; but the best results were observed in Sel-Plex treatment. Therefore, selenium supplementation in the diet of roosters without dexamethasone injection improved total motility, progressive motility, membrane integrity, viability, malondialdehyde, total antioxidant capacity, glutathione peroxidase and superoxide dismutase pre- and post-freezing. It can be concluded, selenium in organic forms in stressed and non-stressed rooster's diet might improve all motility and antioxidant parameters in fresh and frozen-thawed sperm. |
| first_indexed | 2024-04-13T12:31:58Z |
| format | Article |
| id | doaj.art-b5e19a5f9f8c440697732af533d7b63c |
| institution | Directory Open Access Journal |
| issn | 0365-3439 2008-9872 |
| language | English |
| last_indexed | 2024-04-13T12:31:58Z |
| publishDate | 2021-12-01 |
| publisher | Razi Vaccine and Serum Research Institute |
| record_format | Article |
| series | Archives of Razi Institute |
| spelling | doaj.art-b5e19a5f9f8c440697732af533d7b63c2022-12-22T02:46:48ZengRazi Vaccine and Serum Research InstituteArchives of Razi Institute0365-34392008-98722021-12-017661787179510.22092/ari.2021.355960.1765125582Modulation of Negative Effects of Physiological Stress on Frozen-Thawed Semen with Nutrition of Organic Selenium in Ross 308 RoosterN Kamrani0A Karimi1M Nazari2R Masoudi3Department of Animal Science, Faculty of Ahar Agriculture & Natural Resources, University of Tabriz, Tabriz, IranDepartment of Animal Science, Faculty of Ahar Agriculture & Natural Resources, University of Tabriz, Tabriz, IranDepartment of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, IranAnimal Science Research Institute of Iran (ASRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, IranCurrent experiment was carried out in factorial 2×2 arrangement to study the effects of stress (with or without dexamethasone administration) and addition of dietary selenium (with or without selenium supplementation in the diet) in male broiler breeder on the quality of frozen-thawed sperm under oxidative stress induced by dexamethasone. A total of 24 broiler breeder roosters with the age of 28 weeks were used based on a completely randomized design with four therapeutic approaches (factorial 2×2) and six birds in each approach. The experimental treatments were: 1) basal diet without selenium supplementation and injection of saline (CON), 2) basal diet with dexamethasone injection (4 mg/kg BW, three times every other day for one week), (DEX), 3) without dexamethasone injection and supplementation with 0.3 mg/kg selenium (Sel-Plex), and 4) dexamethasone injection and basal diet supplemented with 0.3 mg/kg of diet selenium (Sel-Plex+Dex). Sperm samples were collected from roosters. Motility, progressive motility, plasma membrane integrity, viability, malondialdehyde concentration and antioxidant parameters were evaluated in fresh and frozen-thawed semen. In spite of non-significant interaction effects, factorial analysis indicated the significant effect of every factor on different experimental parameters in fresh and frozen-thawed semen (P<0.05); The results revealed that total and progressive motility, plasma membrane integrity and viability were lower in DEX group when compared with other treatments (P<0.05). On the other hand, malondialdehyde concentration was higher in DEX group in comparison with Con, Sel-Plex and Sel-Plex+DEX groups (P<0.05). Moreover, total antioxidant capacity, level of glutathione peroxidase and superoxide dismutase were lower in DEX group as compared with other treatments (P<0.05). Our findings indicated that administration of selenium in dexamethasone-receiving roosters (Sel-Plex+DEX) improved the parameters of fresh and frozen-thawed sperm; but the best results were observed in Sel-Plex treatment. Therefore, selenium supplementation in the diet of roosters without dexamethasone injection improved total motility, progressive motility, membrane integrity, viability, malondialdehyde, total antioxidant capacity, glutathione peroxidase and superoxide dismutase pre- and post-freezing. It can be concluded, selenium in organic forms in stressed and non-stressed rooster's diet might improve all motility and antioxidant parameters in fresh and frozen-thawed sperm.https://archrazi.areeo.ac.ir/article_125582_ac2a1adae71a683955d9326e7d219020.pdffrozen-thawed spermmotility parametersphysiological stresssel-plex |
| spellingShingle | N Kamrani A Karimi M Nazari R Masoudi Modulation of Negative Effects of Physiological Stress on Frozen-Thawed Semen with Nutrition of Organic Selenium in Ross 308 Rooster Archives of Razi Institute frozen-thawed sperm motility parameters physiological stress sel-plex |
| title | Modulation of Negative Effects of Physiological Stress on Frozen-Thawed Semen with Nutrition of Organic Selenium in Ross 308 Rooster |
| title_full | Modulation of Negative Effects of Physiological Stress on Frozen-Thawed Semen with Nutrition of Organic Selenium in Ross 308 Rooster |
| title_fullStr | Modulation of Negative Effects of Physiological Stress on Frozen-Thawed Semen with Nutrition of Organic Selenium in Ross 308 Rooster |
| title_full_unstemmed | Modulation of Negative Effects of Physiological Stress on Frozen-Thawed Semen with Nutrition of Organic Selenium in Ross 308 Rooster |
| title_short | Modulation of Negative Effects of Physiological Stress on Frozen-Thawed Semen with Nutrition of Organic Selenium in Ross 308 Rooster |
| title_sort | modulation of negative effects of physiological stress on frozen thawed semen with nutrition of organic selenium in ross 308 rooster |
| topic | frozen-thawed sperm motility parameters physiological stress sel-plex |
| url | https://archrazi.areeo.ac.ir/article_125582_ac2a1adae71a683955d9326e7d219020.pdf |
| work_keys_str_mv | AT nkamrani modulationofnegativeeffectsofphysiologicalstressonfrozenthawedsemenwithnutritionoforganicseleniuminross308rooster AT akarimi modulationofnegativeeffectsofphysiologicalstressonfrozenthawedsemenwithnutritionoforganicseleniuminross308rooster AT mnazari modulationofnegativeeffectsofphysiologicalstressonfrozenthawedsemenwithnutritionoforganicseleniuminross308rooster AT rmasoudi modulationofnegativeeffectsofphysiologicalstressonfrozenthawedsemenwithnutritionoforganicseleniuminross308rooster |