A Metabolomics Exploration of the Sexual Phase in the Marine Diatom <i>Pseudo-nitzschia multistriata</i>
<i>Pseudo-nitzschia multistriata</i> is a planktonic marine diatom with a diplontic life cycle comprising a short sexual phase, during which gametes are produced following the encounter of two diploid cells of opposite mating type (MT). Gene expression studies have highlighted the presen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/6/313 |
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| author | Federica Fiorini Camilla Borgonuovo Maria Immacolata Ferrante Mark Brönstrup |
| author_facet | Federica Fiorini Camilla Borgonuovo Maria Immacolata Ferrante Mark Brönstrup |
| author_sort | Federica Fiorini |
| collection | DOAJ |
| description | <i>Pseudo-nitzschia multistriata</i> is a planktonic marine diatom with a diplontic life cycle comprising a short sexual phase, during which gametes are produced following the encounter of two diploid cells of opposite mating type (MT). Gene expression studies have highlighted the presence of substantial changes occurring at the onset of sexual reproduction. Herein, we have hypothesized that the amount and nature of cellular metabolites varies along the mating process. To capture the metabolome of <i>Pseudo-nitzschia multistriata</i> at different harvesting times in an unbiased manner, we undertook an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry. Using three different extraction steps, the method revealed pronounced differences in the metabolic profiles between control cells in the vegetative phase (MT+ and MT−) and mixed strains of opposite MTs (cross) undergoing sexual reproduction. Of the 2408 high-quality features obtained, 70 known metabolites could be identified based on in-house libraries and online databases; additional 46 features could be classified by molecular networking of tandem mass spectra. The reduction of phytol detected in the cross can be linked to the general downregulation of photosynthesis during sexual reproduction observed elsewhere. Moreover, the role of highly regulated compounds such as 7-dehydrodesmosterol, whose changes in abundance were the highest in the experiment, oleamide, ectoine, or trigonelline is discussed. |
| first_indexed | 2024-03-10T19:11:24Z |
| format | Article |
| id | doaj.art-b5e290c6790245b1aa24d833b515d62e |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T19:11:24Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-b5e290c6790245b1aa24d833b515d62e2023-11-20T03:47:38ZengMDPI AGMarine Drugs1660-33972020-06-0118631310.3390/md18060313A Metabolomics Exploration of the Sexual Phase in the Marine Diatom <i>Pseudo-nitzschia multistriata</i>Federica Fiorini0Camilla Borgonuovo1Maria Immacolata Ferrante2Mark Brönstrup3Department of Chemical Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, GermanyIntegrative Marine Ecology Department, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, ItalyIntegrative Marine Ecology Department, Stazione Zoologica Anton Dohrn, Villa Comunale, 80121 Naples, ItalyDepartment of Chemical Biology, Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany<i>Pseudo-nitzschia multistriata</i> is a planktonic marine diatom with a diplontic life cycle comprising a short sexual phase, during which gametes are produced following the encounter of two diploid cells of opposite mating type (MT). Gene expression studies have highlighted the presence of substantial changes occurring at the onset of sexual reproduction. Herein, we have hypothesized that the amount and nature of cellular metabolites varies along the mating process. To capture the metabolome of <i>Pseudo-nitzschia multistriata</i> at different harvesting times in an unbiased manner, we undertook an untargeted metabolomics approach based on liquid chromatography–tandem mass spectrometry. Using three different extraction steps, the method revealed pronounced differences in the metabolic profiles between control cells in the vegetative phase (MT+ and MT−) and mixed strains of opposite MTs (cross) undergoing sexual reproduction. Of the 2408 high-quality features obtained, 70 known metabolites could be identified based on in-house libraries and online databases; additional 46 features could be classified by molecular networking of tandem mass spectra. The reduction of phytol detected in the cross can be linked to the general downregulation of photosynthesis during sexual reproduction observed elsewhere. Moreover, the role of highly regulated compounds such as 7-dehydrodesmosterol, whose changes in abundance were the highest in the experiment, oleamide, ectoine, or trigonelline is discussed.https://www.mdpi.com/1660-3397/18/6/313marine natural productsmetabolomicsmass spectrometrydiatoms<i>Pseudo-nitzschia multistriata</i>sexual reproduction |
| spellingShingle | Federica Fiorini Camilla Borgonuovo Maria Immacolata Ferrante Mark Brönstrup A Metabolomics Exploration of the Sexual Phase in the Marine Diatom <i>Pseudo-nitzschia multistriata</i> Marine Drugs marine natural products metabolomics mass spectrometry diatoms <i>Pseudo-nitzschia multistriata</i> sexual reproduction |
| title | A Metabolomics Exploration of the Sexual Phase in the Marine Diatom <i>Pseudo-nitzschia multistriata</i> |
| title_full | A Metabolomics Exploration of the Sexual Phase in the Marine Diatom <i>Pseudo-nitzschia multistriata</i> |
| title_fullStr | A Metabolomics Exploration of the Sexual Phase in the Marine Diatom <i>Pseudo-nitzschia multistriata</i> |
| title_full_unstemmed | A Metabolomics Exploration of the Sexual Phase in the Marine Diatom <i>Pseudo-nitzschia multistriata</i> |
| title_short | A Metabolomics Exploration of the Sexual Phase in the Marine Diatom <i>Pseudo-nitzschia multistriata</i> |
| title_sort | metabolomics exploration of the sexual phase in the marine diatom i pseudo nitzschia multistriata i |
| topic | marine natural products metabolomics mass spectrometry diatoms <i>Pseudo-nitzschia multistriata</i> sexual reproduction |
| url | https://www.mdpi.com/1660-3397/18/6/313 |
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