Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm
Oil palm (Elaeis guineensis Jacq.) is a tropical woody oil crop of the palm family and is known as “the oil king of the world,” but its palm oil contains about 50% palmitic acid, which is considered unhealthy for humans. Intron polymorphisms (IP) are highly efficient and easily examined molecular ma...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.885418/full |
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| author | Jing Li Yaodong Yang Xiwei Sun Rui Liu Wei Xia Peng Shi Lixia Zhou Yong Wang Yi Wu Xintao Lei Yong Xiao |
| author_facet | Jing Li Yaodong Yang Xiwei Sun Rui Liu Wei Xia Peng Shi Lixia Zhou Yong Wang Yi Wu Xintao Lei Yong Xiao |
| author_sort | Jing Li |
| collection | DOAJ |
| description | Oil palm (Elaeis guineensis Jacq.) is a tropical woody oil crop of the palm family and is known as “the oil king of the world,” but its palm oil contains about 50% palmitic acid, which is considered unhealthy for humans. Intron polymorphisms (IP) are highly efficient and easily examined molecular markers located adjacent to exon regions of functional genes, thus may be associated with targeted trait variation. In order to speed up the breeding of oil palm fatty acid composition, the current study identified a total of 310 introns located within 52 candidate genes involved in fatty acid biosynthesis in the oil palm genome. Based on the intron sequences, 205 primer pairs were designed, 64 of which showed polymorphism among 70 oil palm individuals. Phenotypic variation of fatty acid content in the 70 oil palm individuals was also investigated. Association analysis revealed that 13 IP markers were significantly associated with fatty acid content variation, and these IP markers were located on chromosomes 2, 5, 6, 8, 9, and 10 of oil palm. The development of such IP markers may be useful for the genetic improvement of fatty acid composition in oil palm. |
| first_indexed | 2024-04-12T14:41:50Z |
| format | Article |
| id | doaj.art-b5e295e0d113464dba1bed0bbc53ab6e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T14:41:50Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-b5e295e0d113464dba1bed0bbc53ab6e2022-12-22T03:28:48ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.885418885418Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil PalmJing Li0Yaodong Yang1Xiwei Sun2Rui Liu3Wei Xia4Peng Shi5Lixia Zhou6Yong Wang7Yi Wu8Xintao Lei9Yong Xiao10Hainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, ChinaHainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, ChinaHainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, ChinaHainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, ChinaInstitute of Tropical Agriculture and Forestry, Hainan University, Haikou, ChinaHainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, ChinaHainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, ChinaHainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, ChinaHainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaHainan Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, ChinaOil palm (Elaeis guineensis Jacq.) is a tropical woody oil crop of the palm family and is known as “the oil king of the world,” but its palm oil contains about 50% palmitic acid, which is considered unhealthy for humans. Intron polymorphisms (IP) are highly efficient and easily examined molecular markers located adjacent to exon regions of functional genes, thus may be associated with targeted trait variation. In order to speed up the breeding of oil palm fatty acid composition, the current study identified a total of 310 introns located within 52 candidate genes involved in fatty acid biosynthesis in the oil palm genome. Based on the intron sequences, 205 primer pairs were designed, 64 of which showed polymorphism among 70 oil palm individuals. Phenotypic variation of fatty acid content in the 70 oil palm individuals was also investigated. Association analysis revealed that 13 IP markers were significantly associated with fatty acid content variation, and these IP markers were located on chromosomes 2, 5, 6, 8, 9, and 10 of oil palm. The development of such IP markers may be useful for the genetic improvement of fatty acid composition in oil palm.https://www.frontiersin.org/articles/10.3389/fpls.2022.885418/fullElaeis guineensisfatty acidIP markerscandidate geneassociation analysis |
| spellingShingle | Jing Li Yaodong Yang Xiwei Sun Rui Liu Wei Xia Peng Shi Lixia Zhou Yong Wang Yi Wu Xintao Lei Yong Xiao Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm Frontiers in Plant Science Elaeis guineensis fatty acid IP markers candidate gene association analysis |
| title | Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm |
| title_full | Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm |
| title_fullStr | Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm |
| title_full_unstemmed | Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm |
| title_short | Development of Intron Polymorphism Markers and Their Association With Fatty Acid Component Variation in Oil Palm |
| title_sort | development of intron polymorphism markers and their association with fatty acid component variation in oil palm |
| topic | Elaeis guineensis fatty acid IP markers candidate gene association analysis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.885418/full |
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