The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in mice
Introduction. Pneumonia often occurs secondary to influenza infection and accounts for a large proportion of the morbidity and mortality associated with seasonal and pandemic influenza outbreaks. We previously have shown that vaccination with Virus-like particles (VLPs) containing hemagglutinin (HA)...
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Central Research Institute for Epidemiology
2021-01-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.elpub.ru/jour/article/view/950 |
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| author | I. A. Leneva I. N. Falynskova N. P. Kartashova E. A. Glubokova A. V. Poddubikov O. A. Svitich |
| author_facet | I. A. Leneva I. N. Falynskova N. P. Kartashova E. A. Glubokova A. V. Poddubikov O. A. Svitich |
| author_sort | I. A. Leneva |
| collection | DOAJ |
| description | Introduction. Pneumonia often occurs secondary to influenza infection and accounts for a large proportion of the morbidity and mortality associated with seasonal and pandemic influenza outbreaks. We previously have shown that vaccination with Virus-like particles (VLPs) containing hemagglutinin (HA) of influenza virus reduces mortality caused by bacterial infections after an influenza infections in mice.The aim of this work is to study whether this protective effect may be potentiated by supplementing the HA preparation with the influenza neuraminidase (NA).Materials and methods. We studied the effect of Gag-VLPs with the influenza HA or NA from А/PR/8/34 alone or in combination, in a lethal BALB/c mouse model of S. aureus infection after vaccine-matched or mismatched influenza virus challenge.Results. A cocktail of HA-Gag and NA-Gag-VLPs fully protected from weight loss, mortality and viral replication and significantly reduced the bacterial burden in the lungs of А/PR/8/34 infected animals. Immunization with this cocktail HA-Gag-VLPs 100 ng + NA-Gag-VLPs 20 ng also protected 60% of animals from mortality associated with secondary bacterial S. aureus infection following a heterologous H1N1 influenza virus challenge, and led to the significant protection from weight loss and pulmonary pathogen replication even in the absence of HA-inhibition and NA-inhibition antibodies.Conclusion. Our results indicate that influenza vaccination may improve the outcome of a secondary bacterial pneumonia induced by S. aureus after influenza even when the virus is antigenically different from the vaccine strain. At the same time, in our model, the significance of the immunity to influenza virus HA was prevalent. |
| first_indexed | 2024-12-10T19:07:09Z |
| format | Article |
| id | doaj.art-b5e35603be02458b821f44099ad6c19c |
| institution | Directory Open Access Journal |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| last_indexed | 2024-12-10T19:07:09Z |
| publishDate | 2021-01-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj.art-b5e35603be02458b821f44099ad6c19c2022-12-22T01:36:51ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132021-01-0197656457710.36233/0372-9311-2020-97-6-7696The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in miceI. A. Leneva0I. N. Falynskova1N. P. Kartashova2E. A. Glubokova3A. V. Poddubikov4O. A. Svitich5I.I. Mechnikov Research Institute for Vaccines and SeraI.I. Mechnikov Research Institute for Vaccines and SeraI.I. Mechnikov Research Institute for Vaccines and SeraI.I. Mechnikov Research Institute for Vaccines and SeraI.I. Mechnikov Research Institute for Vaccines and SeraI.I. Mechnikov Research Institute for Vaccines and SeraIntroduction. Pneumonia often occurs secondary to influenza infection and accounts for a large proportion of the morbidity and mortality associated with seasonal and pandemic influenza outbreaks. We previously have shown that vaccination with Virus-like particles (VLPs) containing hemagglutinin (HA) of influenza virus reduces mortality caused by bacterial infections after an influenza infections in mice.The aim of this work is to study whether this protective effect may be potentiated by supplementing the HA preparation with the influenza neuraminidase (NA).Materials and methods. We studied the effect of Gag-VLPs with the influenza HA or NA from А/PR/8/34 alone or in combination, in a lethal BALB/c mouse model of S. aureus infection after vaccine-matched or mismatched influenza virus challenge.Results. A cocktail of HA-Gag and NA-Gag-VLPs fully protected from weight loss, mortality and viral replication and significantly reduced the bacterial burden in the lungs of А/PR/8/34 infected animals. Immunization with this cocktail HA-Gag-VLPs 100 ng + NA-Gag-VLPs 20 ng also protected 60% of animals from mortality associated with secondary bacterial S. aureus infection following a heterologous H1N1 influenza virus challenge, and led to the significant protection from weight loss and pulmonary pathogen replication even in the absence of HA-inhibition and NA-inhibition antibodies.Conclusion. Our results indicate that influenza vaccination may improve the outcome of a secondary bacterial pneumonia induced by S. aureus after influenza even when the virus is antigenically different from the vaccine strain. At the same time, in our model, the significance of the immunity to influenza virus HA was prevalent.https://microbiol.elpub.ru/jour/article/view/950influenza virus<i>staphylococcus aureus</i>vlpsecondary bacterial pneumonia after influenza infection |
| spellingShingle | I. A. Leneva I. N. Falynskova N. P. Kartashova E. A. Glubokova A. V. Poddubikov O. A. Svitich The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in mice Журнал микробиологии, эпидемиологии и иммунобиологии influenza virus <i>staphylococcus aureus</i> vlp secondary bacterial pneumonia after influenza infection |
| title | The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in mice |
| title_full | The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in mice |
| title_fullStr | The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in mice |
| title_full_unstemmed | The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in mice |
| title_short | The study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by <i>S. aureus</i> after influenza infection in mice |
| title_sort | study of neuraminidase immunity in protection against secondary bacterial pneumonia induced by i s aureus i after influenza infection in mice |
| topic | influenza virus <i>staphylococcus aureus</i> vlp secondary bacterial pneumonia after influenza infection |
| url | https://microbiol.elpub.ru/jour/article/view/950 |
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