Magnetic Resonance Imaging Combined with Histological Techniques for Dynamic Assessment of Cytotoxic Edema after Cerebral Ischemia-Reperfusion Injury
Background: Reperfusion therapy after ischemic cerebral stroke may cause cerebral ischemia-reperfusion injury (CIRI), and cerebral edema is an important factor that may aggravate CIRI. Our study aimed to dynamically monitor the development of early cytotoxic edema after CIRI by magnetic resonance im...
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| Format: | Article |
| Language: | English |
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IMR Press
2023-11-01
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| Series: | Journal of Integrative Neuroscience |
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| Online Access: | https://www.imrpress.com/journal/JIN/22/6/10.31083/j.jin2206162 |
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| author | Yuhui Ma Peilun Xiao Chenmeng Liu Haimo Zhang Zhen Li Chen Li Miao Yu Jinfeng Long Lin An Xizhen Wang Xiaoli Wang |
| author_facet | Yuhui Ma Peilun Xiao Chenmeng Liu Haimo Zhang Zhen Li Chen Li Miao Yu Jinfeng Long Lin An Xizhen Wang Xiaoli Wang |
| author_sort | Yuhui Ma |
| collection | DOAJ |
| description | Background: Reperfusion therapy after ischemic cerebral stroke may cause cerebral ischemia-reperfusion injury (CIRI), and cerebral edema is an important factor that may aggravate CIRI. Our study aimed to dynamically monitor the development of early cytotoxic edema after CIRI by magnetic resonance imaging (MRI) and to validate it using multiple histological imaging methods. Methods: Male Sprague Dawley rats were divided into sham and CIRI groups. T2-weighted imaging (T2WI) and diffusion-weighted imaging (DWI)-MRI scans were performed in the sham and CIRI groups after reperfusion. Relative apparent diffusion coefficient (rADC) values were calculated and the midline shift (MLS) was measured. A series of histological detection techniques were performed to observe changes in the cerebral cortex and striatum of CIRI rats. Correlation analysis of rADC values with aquaporin-4 (AQP4) and sodium-potassium-chloride cotransport protein 1 (Na+-K+-2Cl-- cotransporter 1; NKCC1) was performed. Results: rADC values began to increase and reached a relatively low value in the cerebral cortex and striatum at 24 h after reperfusion, and the MLS reached relatively high values at 24 h after reperfusion (all p < 0.05). Hematoxylin-eosin (HE) staining showed that the nerve cells in the cortex and striatum of the sham group were regular in morphology and neatly arranged, and in the CIRI-24 h group were irregular, disorganized, and loosely structured. Using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, the number of TUNEL+ cells in the ischemic cortex and striatum in CIRI-24 h group was shown to increase significantly compared with the sham group (p < 0.05). Transmission electron microscopy showed that the perivascular astrocytic foot processes were swollen in the cortex and striatum of the CIRI-24 h group. Pearson correlation analysis demonstrated that rADC values were negatively correlated with the number of anti-glial fibrillary acidic protein (GFAP)+AQP4+ and GFAP+NKCC1+ cells of the CIRI rats. Conclusions: MRI combined with histological techniques can dynamically assess cytotoxic edema after CIRI, in a manner that is clear and intuitive for scientific researchers and clinicians, and provides a scientific basis for the application of MRI techniques for monitoring the dynamic progress of CIRI. |
| first_indexed | 2024-03-08T16:55:14Z |
| format | Article |
| id | doaj.art-b5e78b398349459aa4b242c65969b59c |
| institution | Directory Open Access Journal |
| issn | 0219-6352 |
| language | English |
| last_indexed | 2024-03-08T16:55:14Z |
| publishDate | 2023-11-01 |
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| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-b5e78b398349459aa4b242c65969b59c2024-01-05T02:39:08ZengIMR PressJournal of Integrative Neuroscience0219-63522023-11-0122616210.31083/j.jin2206162S0219-6352(23)00643-5Magnetic Resonance Imaging Combined with Histological Techniques for Dynamic Assessment of Cytotoxic Edema after Cerebral Ischemia-Reperfusion InjuryYuhui Ma0Peilun Xiao1Chenmeng Liu2Haimo Zhang3Zhen Li4Chen Li5Miao Yu6Jinfeng Long7Lin An8Xizhen Wang9Xiaoli Wang10Medical Imaging Center, Affiliated Hospital of Weifang Medical University, 261053 Weifang, Shandong, ChinaDepartment of Anatomy, School of Basic Medicine, Weifang Medical University, 261053 Weifang, Shandong, ChinaSchool of Medical Imaging, Weifang Medical University, 261053 Weifang, Shandong, ChinaSchool of Medical Imaging, Weifang Medical University, 261053 Weifang, Shandong, ChinaMedical Imaging Center, Affiliated Hospital of Weifang Medical University, 261053 Weifang, Shandong, ChinaSchool of Medical Imaging, Weifang Medical University, 261053 Weifang, Shandong, ChinaSchool of Medical Imaging, Weifang Medical University, 261053 Weifang, Shandong, ChinaSchool of Medical Imaging, Weifang Medical University, 261053 Weifang, Shandong, ChinaSchool of Medical Imaging, Weifang Medical University, 261053 Weifang, Shandong, ChinaMedical Imaging Center, Affiliated Hospital of Weifang Medical University, 261053 Weifang, Shandong, ChinaMedical Imaging Center, Affiliated Hospital of Weifang Medical University, 261053 Weifang, Shandong, ChinaBackground: Reperfusion therapy after ischemic cerebral stroke may cause cerebral ischemia-reperfusion injury (CIRI), and cerebral edema is an important factor that may aggravate CIRI. Our study aimed to dynamically monitor the development of early cytotoxic edema after CIRI by magnetic resonance imaging (MRI) and to validate it using multiple histological imaging methods. Methods: Male Sprague Dawley rats were divided into sham and CIRI groups. T2-weighted imaging (T2WI) and diffusion-weighted imaging (DWI)-MRI scans were performed in the sham and CIRI groups after reperfusion. Relative apparent diffusion coefficient (rADC) values were calculated and the midline shift (MLS) was measured. A series of histological detection techniques were performed to observe changes in the cerebral cortex and striatum of CIRI rats. Correlation analysis of rADC values with aquaporin-4 (AQP4) and sodium-potassium-chloride cotransport protein 1 (Na+-K+-2Cl-- cotransporter 1; NKCC1) was performed. Results: rADC values began to increase and reached a relatively low value in the cerebral cortex and striatum at 24 h after reperfusion, and the MLS reached relatively high values at 24 h after reperfusion (all p < 0.05). Hematoxylin-eosin (HE) staining showed that the nerve cells in the cortex and striatum of the sham group were regular in morphology and neatly arranged, and in the CIRI-24 h group were irregular, disorganized, and loosely structured. Using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining, the number of TUNEL+ cells in the ischemic cortex and striatum in CIRI-24 h group was shown to increase significantly compared with the sham group (p < 0.05). Transmission electron microscopy showed that the perivascular astrocytic foot processes were swollen in the cortex and striatum of the CIRI-24 h group. Pearson correlation analysis demonstrated that rADC values were negatively correlated with the number of anti-glial fibrillary acidic protein (GFAP)+AQP4+ and GFAP+NKCC1+ cells of the CIRI rats. Conclusions: MRI combined with histological techniques can dynamically assess cytotoxic edema after CIRI, in a manner that is clear and intuitive for scientific researchers and clinicians, and provides a scientific basis for the application of MRI techniques for monitoring the dynamic progress of CIRI.https://www.imrpress.com/journal/JIN/22/6/10.31083/j.jin2206162cerebral ischemia-reperfusion injurystrokemagnetic resonance imagingcytotoxic edemarat |
| spellingShingle | Yuhui Ma Peilun Xiao Chenmeng Liu Haimo Zhang Zhen Li Chen Li Miao Yu Jinfeng Long Lin An Xizhen Wang Xiaoli Wang Magnetic Resonance Imaging Combined with Histological Techniques for Dynamic Assessment of Cytotoxic Edema after Cerebral Ischemia-Reperfusion Injury Journal of Integrative Neuroscience cerebral ischemia-reperfusion injury stroke magnetic resonance imaging cytotoxic edema rat |
| title | Magnetic Resonance Imaging Combined with Histological Techniques for Dynamic Assessment of Cytotoxic Edema after Cerebral Ischemia-Reperfusion Injury |
| title_full | Magnetic Resonance Imaging Combined with Histological Techniques for Dynamic Assessment of Cytotoxic Edema after Cerebral Ischemia-Reperfusion Injury |
| title_fullStr | Magnetic Resonance Imaging Combined with Histological Techniques for Dynamic Assessment of Cytotoxic Edema after Cerebral Ischemia-Reperfusion Injury |
| title_full_unstemmed | Magnetic Resonance Imaging Combined with Histological Techniques for Dynamic Assessment of Cytotoxic Edema after Cerebral Ischemia-Reperfusion Injury |
| title_short | Magnetic Resonance Imaging Combined with Histological Techniques for Dynamic Assessment of Cytotoxic Edema after Cerebral Ischemia-Reperfusion Injury |
| title_sort | magnetic resonance imaging combined with histological techniques for dynamic assessment of cytotoxic edema after cerebral ischemia reperfusion injury |
| topic | cerebral ischemia-reperfusion injury stroke magnetic resonance imaging cytotoxic edema rat |
| url | https://www.imrpress.com/journal/JIN/22/6/10.31083/j.jin2206162 |
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