Effects of High-Fat Diet on the Gut Microbiota of Renalase Gene Knockout Mice
Metabolic diseases caused by gene and unhealthy living habits are increasing, which seriously threaten the life of people worldwide. Moreover, the microbiome has been shown to play an active role in the prevention and treatment of metabolic diseases. However, reliable evidence on renalase gene (Rnls...
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MDPI AG
2022-09-01
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| author | Hui Fang Kai Aoki Katsuyuki Tokinoya Masato Yonamine Takehito Sugasawa Yasushi Kawakami Kazuhiro Takekoshi |
| author_facet | Hui Fang Kai Aoki Katsuyuki Tokinoya Masato Yonamine Takehito Sugasawa Yasushi Kawakami Kazuhiro Takekoshi |
| author_sort | Hui Fang |
| collection | DOAJ |
| description | Metabolic diseases caused by gene and unhealthy living habits are increasing, which seriously threaten the life of people worldwide. Moreover, the microbiome has been shown to play an active role in the prevention and treatment of metabolic diseases. However, reliable evidence on renalase gene (Rnls), as a common gene related to metabolic diseases, is still lacking with regard to the influence on the microbiome. Hence, we investigated the effect of a normal diet (ND) and a high-fat diet (HFD) on the gut microbiota of Rnls knockout (Rnls<sup>−/−</sup>) and wild-type (Rnls<sup>+/+</sup>) mice. At the end of the 8-week experiment, DNA samples were extracted from fresh feces, and the composition of microbiota was profiled. The species in Rnls<sup>+/+</sup>-ND group were <i>Bifidobacterium pseudolongum</i> and <i>Lactobacillus reuteri</i>. Conversely, the species in Rnls<sup>−/−</sup>-ND group belonged to the genera <i>Lactobacillus</i> and <i>Turicibacter</i>. The HFD changed the ratio of Firmicutes/Bacteroidetes; while the bacteria in the Rnls<sup>+/+</sup>-HFD and Rnls<sup>−/−</sup>-HFD groups were different. Overall, this study not only revealed the composition of microbiota in Rnls<sup>−/−</sup> mice, but also indicated that Rnls and the bacteria related to Rnls may be new candidates in the prevention and diagnosis of metabolic diseases at an early stage. |
| first_indexed | 2024-03-09T22:54:30Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2673-4168 |
| language | English |
| last_indexed | 2024-03-09T22:54:30Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Obesities |
| spelling | doaj.art-b5e9a61e63784364bd721fed92e5b2de2023-11-23T18:13:53ZengMDPI AGObesities2673-41682022-09-012330331610.3390/obesities2030025Effects of High-Fat Diet on the Gut Microbiota of Renalase Gene Knockout MiceHui Fang0Kai Aoki1Katsuyuki Tokinoya2Masato Yonamine3Takehito Sugasawa4Yasushi Kawakami5Kazuhiro Takekoshi6Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba 305-8577, JapanFaculty of Medicine, University of Tsukuba, Tsukuba 305-8577, JapanJapan Society for the Promotion of Science, Kojimachi Business Center Building, Tokyo 102-0083, JapanFaculty of Medicine, University of Tsukuba, Tsukuba 305-8577, JapanFaculty of Medicine, University of Tsukuba, Tsukuba 305-8577, JapanFaculty of Medicine, University of Tsukuba, Tsukuba 305-8577, JapanFaculty of Medicine, University of Tsukuba, Tsukuba 305-8577, JapanMetabolic diseases caused by gene and unhealthy living habits are increasing, which seriously threaten the life of people worldwide. Moreover, the microbiome has been shown to play an active role in the prevention and treatment of metabolic diseases. However, reliable evidence on renalase gene (Rnls), as a common gene related to metabolic diseases, is still lacking with regard to the influence on the microbiome. Hence, we investigated the effect of a normal diet (ND) and a high-fat diet (HFD) on the gut microbiota of Rnls knockout (Rnls<sup>−/−</sup>) and wild-type (Rnls<sup>+/+</sup>) mice. At the end of the 8-week experiment, DNA samples were extracted from fresh feces, and the composition of microbiota was profiled. The species in Rnls<sup>+/+</sup>-ND group were <i>Bifidobacterium pseudolongum</i> and <i>Lactobacillus reuteri</i>. Conversely, the species in Rnls<sup>−/−</sup>-ND group belonged to the genera <i>Lactobacillus</i> and <i>Turicibacter</i>. The HFD changed the ratio of Firmicutes/Bacteroidetes; while the bacteria in the Rnls<sup>+/+</sup>-HFD and Rnls<sup>−/−</sup>-HFD groups were different. Overall, this study not only revealed the composition of microbiota in Rnls<sup>−/−</sup> mice, but also indicated that Rnls and the bacteria related to Rnls may be new candidates in the prevention and diagnosis of metabolic diseases at an early stage.https://www.mdpi.com/2673-4168/2/3/25Rnls knockoutnormal diethigh-fat dietgut microbiotaFirmicutes/Bacteroidetes Tukey’s post hoc test |
| spellingShingle | Hui Fang Kai Aoki Katsuyuki Tokinoya Masato Yonamine Takehito Sugasawa Yasushi Kawakami Kazuhiro Takekoshi Effects of High-Fat Diet on the Gut Microbiota of Renalase Gene Knockout Mice Obesities Rnls knockout normal diet high-fat diet gut microbiota Firmicutes/Bacteroidetes Tukey’s post hoc test |
| title | Effects of High-Fat Diet on the Gut Microbiota of Renalase Gene Knockout Mice |
| title_full | Effects of High-Fat Diet on the Gut Microbiota of Renalase Gene Knockout Mice |
| title_fullStr | Effects of High-Fat Diet on the Gut Microbiota of Renalase Gene Knockout Mice |
| title_full_unstemmed | Effects of High-Fat Diet on the Gut Microbiota of Renalase Gene Knockout Mice |
| title_short | Effects of High-Fat Diet on the Gut Microbiota of Renalase Gene Knockout Mice |
| title_sort | effects of high fat diet on the gut microbiota of renalase gene knockout mice |
| topic | Rnls knockout normal diet high-fat diet gut microbiota Firmicutes/Bacteroidetes Tukey’s post hoc test |
| url | https://www.mdpi.com/2673-4168/2/3/25 |
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