Investigating the L-asparaginase Production in the Yeast Yarrowia Lipolytica
Introduction: L-Asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid and ammonia. This enzyme is used for the treatment of patients with acute lymphoblastic leukemia, melanosarcoma and lymphosarcoma. It is also used in the production of acrylamide-free foods. Considering the impo...
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| Format: | Article |
| Language: | English |
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University of Isfahan
2018-09-01
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| Series: | Biological Journal of Microorganism |
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| Online Access: | http://bjm.ui.ac.ir/article_22966_09fa94a50f8cdda018b3f2164b844159.pdf |
| _version_ | 1818990104356585472 |
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| author | Farshad Darvishi Fereshteh Shamsi |
| author_facet | Farshad Darvishi Fereshteh Shamsi |
| author_sort | Farshad Darvishi |
| collection | DOAJ |
| description | Introduction: L-Asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid and ammonia. This enzyme is used for the treatment of patients with acute lymphoblastic leukemia, melanosarcoma and lymphosarcoma. It is also used in the production of acrylamide-free foods. Considering the important applications of this enzyme, the aim of this study was to introduce a new microbial source for the production of eukaryotic L-asparaginase. Materials and Methods: The quality and quantity production of L-asparaginase was investigated in four strains of yeast Yarrowia lipolytica. The quality assessment of L-asparaginase production was done on asparagine minimal agar. Quantitative assay of the enzyme was done in Czapex Dox’s medium by spectrophotometric method using Nessler’s reagent. Results: All of four strains show that they are able to produce asparaginase making a purple halo around the colony. Y. lipolytica DSM3286, DSM70562, DSM3218 and CBS6303 were produced 17/14, 11, 6/98 and 5/61 U/mL of asparaginase in Czapex Dox’s medium after 24 h, respectively. Discussion and Conclusion: Y. lipolytica DSM3286 was selected as the best asparaginase producer strain with the largest halo and the highest asparaginase production. The Y. lipolytica could be used as a potential source of L-asparaginase production. |
| first_indexed | 2024-12-20T19:49:04Z |
| format | Article |
| id | doaj.art-b5e9eb672c2e45f999c4ae0e696cf593 |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-12-20T19:49:04Z |
| publishDate | 2018-09-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-b5e9eb672c2e45f999c4ae0e696cf5932022-12-21T19:28:21ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812018-09-01727737910.22108/bjm.2018.111339.113322966Investigating the L-asparaginase Production in the Yeast Yarrowia LipolyticaFarshad Darvishi0Fereshteh Shamsi1Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran.Department of Microbiology, Faculty of Science, University of Maragheh, Maragheh, Iran.Introduction: L-Asparaginase catalyzes the hydrolysis of L-asparagine to L-aspartic acid and ammonia. This enzyme is used for the treatment of patients with acute lymphoblastic leukemia, melanosarcoma and lymphosarcoma. It is also used in the production of acrylamide-free foods. Considering the important applications of this enzyme, the aim of this study was to introduce a new microbial source for the production of eukaryotic L-asparaginase. Materials and Methods: The quality and quantity production of L-asparaginase was investigated in four strains of yeast Yarrowia lipolytica. The quality assessment of L-asparaginase production was done on asparagine minimal agar. Quantitative assay of the enzyme was done in Czapex Dox’s medium by spectrophotometric method using Nessler’s reagent. Results: All of four strains show that they are able to produce asparaginase making a purple halo around the colony. Y. lipolytica DSM3286, DSM70562, DSM3218 and CBS6303 were produced 17/14, 11, 6/98 and 5/61 U/mL of asparaginase in Czapex Dox’s medium after 24 h, respectively. Discussion and Conclusion: Y. lipolytica DSM3286 was selected as the best asparaginase producer strain with the largest halo and the highest asparaginase production. The Y. lipolytica could be used as a potential source of L-asparaginase production.http://bjm.ui.ac.ir/article_22966_09fa94a50f8cdda018b3f2164b844159.pdfYarrowia LipolyticaAsparaginaseAnticancer DrugProduction. |
| spellingShingle | Farshad Darvishi Fereshteh Shamsi Investigating the L-asparaginase Production in the Yeast Yarrowia Lipolytica Biological Journal of Microorganism Yarrowia Lipolytica Asparaginase Anticancer Drug Production. |
| title | Investigating the L-asparaginase Production in the Yeast Yarrowia Lipolytica |
| title_full | Investigating the L-asparaginase Production in the Yeast Yarrowia Lipolytica |
| title_fullStr | Investigating the L-asparaginase Production in the Yeast Yarrowia Lipolytica |
| title_full_unstemmed | Investigating the L-asparaginase Production in the Yeast Yarrowia Lipolytica |
| title_short | Investigating the L-asparaginase Production in the Yeast Yarrowia Lipolytica |
| title_sort | investigating the l asparaginase production in the yeast yarrowia lipolytica |
| topic | Yarrowia Lipolytica Asparaginase Anticancer Drug Production. |
| url | http://bjm.ui.ac.ir/article_22966_09fa94a50f8cdda018b3f2164b844159.pdf |
| work_keys_str_mv | AT farshaddarvishi investigatingthelasparaginaseproductionintheyeastyarrowialipolytica AT fereshtehshamsi investigatingthelasparaginaseproductionintheyeastyarrowialipolytica |