Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine sera
Porcine circovirosis is an infectious-contagious syndrome caused by porcine circovirus type 2 (PCV-2) found mainly in recently weaned piglets causing dermatitis, neurological and reproductive disorders, pneumonia and encephalitis. The objectives of the present study were to validate a Polymerase Cha...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidad de La Salle
2007-12-01
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| Series: | Revista de Medicina Veterinaria |
| Subjects: | |
| Online Access: | http://revistas.lasalle.edu.co/index.php/mv/article/view/1798 |
| Summary: | Porcine circovirosis is an infectious-contagious syndrome caused by porcine circovirus type 2 (PCV-2) found mainly in recently weaned piglets causing dermatitis, neurological and reproductive disorders, pneumonia and encephalitis. The objectives of the present study were to validate a Polymerase Chain Reaction (PCR) technique to detect PCV-2 in swine serum and to apply the validated technique in swine serum samples to detect PCV-2. After the application of two different PCRs, 100% of the surveyed animals were negative to PCV-2; furthermore, the PCR targeted to a region between ORFs 1 and 2 of the virus was found more sensitive when compared to another PCR targeted to the capsid protein gene. As a conclusion, PCR is a valid technique to detect PCV-2 in swine serum and the surveyed population was free of the virus. |
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| ISSN: | 0122-9354 2389-8526 |