Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine sera
Porcine circovirosis is an infectious-contagious syndrome caused by porcine circovirus type 2 (PCV-2) found mainly in recently weaned piglets causing dermatitis, neurological and reproductive disorders, pneumonia and encephalitis. The objectives of the present study were to validate a Polymerase Cha...
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| Format: | Article |
| Language: | English |
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Universidad de La Salle
2007-12-01
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| Series: | Revista de Medicina Veterinaria |
| Subjects: | |
| Online Access: | http://revistas.lasalle.edu.co/index.php/mv/article/view/1798 |
| _version_ | 1818910806025175040 |
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| author | Luisa Fernanda Villadiego Marmolejo Laura Y. Villarreal Buitrago Leonardo J. Richtzenhain Paulo E. Brandão |
| author_facet | Luisa Fernanda Villadiego Marmolejo Laura Y. Villarreal Buitrago Leonardo J. Richtzenhain Paulo E. Brandão |
| author_sort | Luisa Fernanda Villadiego Marmolejo |
| collection | DOAJ |
| description | Porcine circovirosis is an infectious-contagious syndrome caused by porcine circovirus type 2 (PCV-2) found mainly in recently weaned piglets causing dermatitis, neurological and reproductive disorders, pneumonia and encephalitis. The objectives of the present study were to validate a Polymerase Chain Reaction (PCR) technique to detect PCV-2 in swine serum and to apply the validated technique in swine serum samples to detect PCV-2. After the application of two different PCRs, 100% of the surveyed animals were negative to PCV-2; furthermore, the PCR targeted to a region between ORFs 1 and 2 of the virus was found more sensitive when compared to another PCR targeted to the capsid protein gene. As a conclusion, PCR is a valid technique to detect PCV-2 in swine serum and the surveyed population was free of the virus. |
| first_indexed | 2024-12-19T22:48:39Z |
| format | Article |
| id | doaj.art-b5eab44bac244eb88bc9e002a598f12e |
| institution | Directory Open Access Journal |
| issn | 0122-9354 2389-8526 |
| language | English |
| last_indexed | 2024-12-19T22:48:39Z |
| publishDate | 2007-12-01 |
| publisher | Universidad de La Salle |
| record_format | Article |
| series | Revista de Medicina Veterinaria |
| spelling | doaj.art-b5eab44bac244eb88bc9e002a598f12e2022-12-21T20:02:54ZengUniversidad de La SalleRevista de Medicina Veterinaria0122-93542389-85262007-12-0101471510.19052/mv.17981675Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine seraLuisa Fernanda Villadiego MarmolejoLaura Y. Villarreal BuitragoLeonardo J. RichtzenhainPaulo E. BrandãoPorcine circovirosis is an infectious-contagious syndrome caused by porcine circovirus type 2 (PCV-2) found mainly in recently weaned piglets causing dermatitis, neurological and reproductive disorders, pneumonia and encephalitis. The objectives of the present study were to validate a Polymerase Chain Reaction (PCR) technique to detect PCV-2 in swine serum and to apply the validated technique in swine serum samples to detect PCV-2. After the application of two different PCRs, 100% of the surveyed animals were negative to PCV-2; furthermore, the PCR targeted to a region between ORFs 1 and 2 of the virus was found more sensitive when compared to another PCR targeted to the capsid protein gene. As a conclusion, PCR is a valid technique to detect PCV-2 in swine serum and the surveyed population was free of the virus.http://revistas.lasalle.edu.co/index.php/mv/article/view/1798circovirus porcino tipo 2PCRsuero |
| spellingShingle | Luisa Fernanda Villadiego Marmolejo Laura Y. Villarreal Buitrago Leonardo J. Richtzenhain Paulo E. Brandão Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine sera Revista de Medicina Veterinaria circovirus porcino tipo 2 PCR suero |
| title | Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine sera |
| title_full | Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine sera |
| title_fullStr | Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine sera |
| title_full_unstemmed | Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine sera |
| title_short | Validation and aplication of a polymerase chain reaction (PCR) to detect porcine circovirus type 2 (PCV-2) in swine sera |
| title_sort | validation and aplication of a polymerase chain reaction pcr to detect porcine circovirus type 2 pcv 2 in swine sera |
| topic | circovirus porcino tipo 2 PCR suero |
| url | http://revistas.lasalle.edu.co/index.php/mv/article/view/1798 |
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