Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children
Background: Uropathogenic <i>Escherichia coli</i> (UPEC) has increased the incidence of urinary tract infection (UTI). It is the cause of more than 80% of community-acquired cystitis cases and more than 70% of uncomplicated acute pyelonephritis cases. Aim: The present study describes the...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2299 |
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| author | Laura M. Contreras-Alvarado Sergio Zavala-Vega Ariadnna Cruz-Córdova Juan Pablo Reyes-Grajeda Gerardo Escalona-Venegas Víctor Flores Virginia Alcázar-López José Arellano-Galindo Rigoberto Hernández-Castro Graciela Castro-Escarpulli Juan Xicohtencatl-Cortes Sara A. Ochoa |
| author_facet | Laura M. Contreras-Alvarado Sergio Zavala-Vega Ariadnna Cruz-Córdova Juan Pablo Reyes-Grajeda Gerardo Escalona-Venegas Víctor Flores Virginia Alcázar-López José Arellano-Galindo Rigoberto Hernández-Castro Graciela Castro-Escarpulli Juan Xicohtencatl-Cortes Sara A. Ochoa |
| author_sort | Laura M. Contreras-Alvarado |
| collection | DOAJ |
| description | Background: Uropathogenic <i>Escherichia coli</i> (UPEC) has increased the incidence of urinary tract infection (UTI). It is the cause of more than 80% of community-acquired cystitis cases and more than 70% of uncomplicated acute pyelonephritis cases. Aim: The present study describes the molecular epidemiology of UPEC O25b clinical strains based on their resistance profiles, virulence genes, and genetic diversity. Methods: Resistance profiles were identified using the Kirby–Bauer method, including the phenotypic production of extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs). The UPEC serogroups, phylogenetic groups, virulence genes, and integrons were determined via multiplex PCR. Genetic diversity was established using pulsed-field gel electrophoresis (PFGE), and sequence type (ST) was determined via multilocus sequence typing (MLST). Results: UPEC strains (<i>n</i> = 126) from hospitalized children with complicated UTIs (cUTIs) were identified as O25b, of which 41.27% were multidrug resistant (MDR) and 15.87% were extensively drug resistant (XDR). The O25b strains harbored the <i>fimH</i> (95.23%), <i>csgA</i> (91.26%), <i>papG</i>II (80.95%), <i>chuA</i> (95.23%), <i>iutD</i> (88.09%), <i>satA</i> (84.92%), and <i>intl1</i> (47.61%) genes. Moreover, 64.28% were producers of ESBLs and had high genetic diversity. ST131 (63.63%) was associated primarily with phylogenetic group B2, and ST69 (100%) was associated primarily with phylogenetic group D. Conclusion: UPEC O25b/ST131 harbors a wide genetic diversity of virulence and resistance genes, which contribute to cUTIs in pediatrics. |
| first_indexed | 2024-03-10T05:16:06Z |
| format | Article |
| id | doaj.art-b5eba9a88ff54e6c803246ecbb108e5f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:16:06Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-b5eba9a88ff54e6c803246ecbb108e5f2023-11-23T00:29:19ZengMDPI AGMicroorganisms2076-26072021-11-01911229910.3390/microorganisms9112299Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in ChildrenLaura M. Contreras-Alvarado0Sergio Zavala-Vega1Ariadnna Cruz-Córdova2Juan Pablo Reyes-Grajeda3Gerardo Escalona-Venegas4Víctor Flores5Virginia Alcázar-López6José Arellano-Galindo7Rigoberto Hernández-Castro8Graciela Castro-Escarpulli9Juan Xicohtencatl-Cortes10Sara A. Ochoa11Laboratorio de Investigación en Bacteriología Intestinal, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoLaboratorio de Neuropatología, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Ciudad de México 14269, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoSubdirección de Desarrollo de Aplicaciones Clínicas, Instituto Nacional de Medicina Genómica, Ciudad de México 14610, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoDepartment of Biochemistry, University of Cambridge, 80 Tennis Court Road, Cambridge CB2 1GA, UKDepartamento de Laboratorio Clínico, Laboratorio Central, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoLaboratorio de Virología Clínica y Experimental, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoDepartamento de Ecología de Agentes Patógenos, Hospital General Dr. Manuel Gea González, Ciudad de México 4800, MexicoLaboratorio de Investigación Clínica y Ambiental, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México 11340, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoLaboratorio de Investigación en Bacteriología Intestinal, Unidad de Investigación en Enfermedades Infecciosas, Hospital Infantil de México Federico Gómez, Ciudad de México 06720, MexicoBackground: Uropathogenic <i>Escherichia coli</i> (UPEC) has increased the incidence of urinary tract infection (UTI). It is the cause of more than 80% of community-acquired cystitis cases and more than 70% of uncomplicated acute pyelonephritis cases. Aim: The present study describes the molecular epidemiology of UPEC O25b clinical strains based on their resistance profiles, virulence genes, and genetic diversity. Methods: Resistance profiles were identified using the Kirby–Bauer method, including the phenotypic production of extended-spectrum β-lactamases (ESBLs) and metallo-β-lactamases (MBLs). The UPEC serogroups, phylogenetic groups, virulence genes, and integrons were determined via multiplex PCR. Genetic diversity was established using pulsed-field gel electrophoresis (PFGE), and sequence type (ST) was determined via multilocus sequence typing (MLST). Results: UPEC strains (<i>n</i> = 126) from hospitalized children with complicated UTIs (cUTIs) were identified as O25b, of which 41.27% were multidrug resistant (MDR) and 15.87% were extensively drug resistant (XDR). The O25b strains harbored the <i>fimH</i> (95.23%), <i>csgA</i> (91.26%), <i>papG</i>II (80.95%), <i>chuA</i> (95.23%), <i>iutD</i> (88.09%), <i>satA</i> (84.92%), and <i>intl1</i> (47.61%) genes. Moreover, 64.28% were producers of ESBLs and had high genetic diversity. ST131 (63.63%) was associated primarily with phylogenetic group B2, and ST69 (100%) was associated primarily with phylogenetic group D. Conclusion: UPEC O25b/ST131 harbors a wide genetic diversity of virulence and resistance genes, which contribute to cUTIs in pediatrics.https://www.mdpi.com/2076-2607/9/11/2299UPEC O25MDRMLSTgenetic diversity |
| spellingShingle | Laura M. Contreras-Alvarado Sergio Zavala-Vega Ariadnna Cruz-Córdova Juan Pablo Reyes-Grajeda Gerardo Escalona-Venegas Víctor Flores Virginia Alcázar-López José Arellano-Galindo Rigoberto Hernández-Castro Graciela Castro-Escarpulli Juan Xicohtencatl-Cortes Sara A. Ochoa Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children Microorganisms UPEC O25 MDR MLST genetic diversity |
| title | Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children |
| title_full | Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children |
| title_fullStr | Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children |
| title_full_unstemmed | Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children |
| title_short | Molecular Epidemiology of Multidrug-Resistant Uropathogenic <i>Escherichia coli</i> O25b Strains Associated with Complicated Urinary Tract Infection in Children |
| title_sort | molecular epidemiology of multidrug resistant uropathogenic i escherichia coli i o25b strains associated with complicated urinary tract infection in children |
| topic | UPEC O25 MDR MLST genetic diversity |
| url | https://www.mdpi.com/2076-2607/9/11/2299 |
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