The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity.
Endothelial cells (ECs) are active participants of an inflammatory process in glomeruli. EC damage has been shown to play an important role in the progression of glomerulonephritis (GN). The degree of glomerular and peritubular capillary loss in models of progressive renal disease correlates with th...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Via Medica
2010-08-01
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| Series: | Folia Histochemica et Cytobiologica |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4237 |
| _version_ | 1818950756573642752 |
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| author | Flaviu Bob Gheorghe Bozdog Silvia Velciov Ligia Petrica Virginia Trandafirescu Diana Herman Elena Potencz Gheorghe Gluhovschi Cristina Gluhovschi Corina Vernic Daniel Cioca |
| author_facet | Flaviu Bob Gheorghe Bozdog Silvia Velciov Ligia Petrica Virginia Trandafirescu Diana Herman Elena Potencz Gheorghe Gluhovschi Cristina Gluhovschi Corina Vernic Daniel Cioca |
| author_sort | Flaviu Bob |
| collection | DOAJ |
| description | Endothelial cells (ECs) are active participants of an inflammatory process in glomeruli. EC damage has been shown to play an important role in the progression of glomerulonephritis (GN). The degree of glomerular and peritubular capillary loss in models of progressive renal disease correlates with the severity of glomerulosclerosis and interstitial fibrosis. The aim of our study was to analyze the association of vWF, CD31 and CD34 immunoreactivity with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity in GN. A cross-sectional study of 22 patients with GN was conducted. Conventional stains (hematoxylin-eosin, periodic acid Schiff and Trichrome GĂśmĂśri stains) and immunohistochemistry (vWF, CD31 and CD34) were employed on kidney biopsies. Activity and chronicity of GN, as well as glomerular segmental sclerosis and interstitial fibrosis, were evaluated according to a scoring system initially used for lupus nephritis and antineutrophil-cytoplasmic-antibody-associated vasculitis. Immunohistochemistry was assessed using a semi-quantitative score. Statistical analysis was performed using EpiInfo 6.04. The mean patient age was 46.68+/-14.09; 14 patients were male, and eight were female. Performing Spearman's rank correlation test, no correlation was found between each marker and glomerular segmental sclerosis, interstitial fibrosis, activity and chronicity, which suggests a loss of these markers and microvasculature involvement. |
| first_indexed | 2024-12-20T09:23:39Z |
| format | Article |
| id | doaj.art-b5ee00862ea84583894589e3fefd1b57 |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-12-20T09:23:39Z |
| publishDate | 2010-08-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-b5ee00862ea84583894589e3fefd1b572022-12-21T19:45:14ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312010-08-0148223023610.5603/4237The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity.Flaviu BobGheorghe BozdogSilvia VelciovLigia PetricaVirginia TrandafirescuDiana HermanElena PotenczGheorghe GluhovschiCristina GluhovschiCorina VernicDaniel CiocaEndothelial cells (ECs) are active participants of an inflammatory process in glomeruli. EC damage has been shown to play an important role in the progression of glomerulonephritis (GN). The degree of glomerular and peritubular capillary loss in models of progressive renal disease correlates with the severity of glomerulosclerosis and interstitial fibrosis. The aim of our study was to analyze the association of vWF, CD31 and CD34 immunoreactivity with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity in GN. A cross-sectional study of 22 patients with GN was conducted. Conventional stains (hematoxylin-eosin, periodic acid Schiff and Trichrome GĂśmĂśri stains) and immunohistochemistry (vWF, CD31 and CD34) were employed on kidney biopsies. Activity and chronicity of GN, as well as glomerular segmental sclerosis and interstitial fibrosis, were evaluated according to a scoring system initially used for lupus nephritis and antineutrophil-cytoplasmic-antibody-associated vasculitis. Immunohistochemistry was assessed using a semi-quantitative score. Statistical analysis was performed using EpiInfo 6.04. The mean patient age was 46.68+/-14.09; 14 patients were male, and eight were female. Performing Spearman's rank correlation test, no correlation was found between each marker and glomerular segmental sclerosis, interstitial fibrosis, activity and chronicity, which suggests a loss of these markers and microvasculature involvement.http://czasopisma.viamedica.pl/fhc/article/view/4237 |
| spellingShingle | Flaviu Bob Gheorghe Bozdog Silvia Velciov Ligia Petrica Virginia Trandafirescu Diana Herman Elena Potencz Gheorghe Gluhovschi Cristina Gluhovschi Corina Vernic Daniel Cioca The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity. Folia Histochemica et Cytobiologica |
| title | The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity. |
| title_full | The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity. |
| title_fullStr | The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity. |
| title_full_unstemmed | The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity. |
| title_short | The endothelial cell markers von Willebrand Factor (vWF), CD31 and CD34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis, interstitial fibrosis, activity and chronicity. |
| title_sort | endothelial cell markers von willebrand factor vwf cd31 and cd34 are lost in glomerulonephritis and no longer correlate with the morphological indices of glomerular sclerosis interstitial fibrosis activity and chronicity |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4237 |
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