Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions
Abstract Objective Adhesion of cells to the extracellular matrix is facilitated by integrin receptors. We recently found that a nanoscale organization of plasma membrane located integrins containing the β1 subunit is responsible for an enhanced radio-resistance in 3D cultured cells over cells grown...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | BMC Research Notes |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13104-017-3032-8 |
| _version_ | 1818242484359135232 |
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| author | Laura Babel Larissa Kruse Steven Bump Markus Langhans Tobias Meckel |
| author_facet | Laura Babel Larissa Kruse Steven Bump Markus Langhans Tobias Meckel |
| author_sort | Laura Babel |
| collection | DOAJ |
| description | Abstract Objective Adhesion of cells to the extracellular matrix is facilitated by integrin receptors. We recently found that a nanoscale organization of plasma membrane located integrins containing the β1 subunit is responsible for an enhanced radio-resistance in 3D cultured cells over cells grown in 2D. While ionizing radiation is known to have broad effects on the lipid composition of the plasma membrane and their organization in lipid-rafts, it is not clear whether the effects of ionizing radiation on the nanoscale clustering of integrins is lipid-raft dependent. Results Using single molecule microscopy we can show that β1 integrins colocalize with cholesterol in lipid-rafts. Ionizing radiation, as an extrinsic stressor, causes the separation of β1 integrins from cholesterol lipid raft suggesting that the effects of ionizing radiation on the clustering of β1 integrins are lipid-raft independent. |
| first_indexed | 2024-12-12T13:45:58Z |
| format | Article |
| id | doaj.art-b5ee7ea15c8543d6b131a38cafd83806 |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-12T13:45:58Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-b5ee7ea15c8543d6b131a38cafd838062022-12-22T00:22:41ZengBMCBMC Research Notes1756-05002017-12-011011610.1186/s13104-017-3032-8Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesionsLaura Babel0Larissa Kruse1Steven Bump2Markus Langhans3Tobias Meckel4Membrane Dynamics, Department of Biology, Technische Universität DarmstadtMembrane Dynamics, Department of Biology, Technische Universität DarmstadtMembrane Dynamics, Department of Biology, Technische Universität DarmstadtMembrane Dynamics, Department of Biology, Technische Universität DarmstadtMembrane Dynamics, Department of Biology, Technische Universität DarmstadtAbstract Objective Adhesion of cells to the extracellular matrix is facilitated by integrin receptors. We recently found that a nanoscale organization of plasma membrane located integrins containing the β1 subunit is responsible for an enhanced radio-resistance in 3D cultured cells over cells grown in 2D. While ionizing radiation is known to have broad effects on the lipid composition of the plasma membrane and their organization in lipid-rafts, it is not clear whether the effects of ionizing radiation on the nanoscale clustering of integrins is lipid-raft dependent. Results Using single molecule microscopy we can show that β1 integrins colocalize with cholesterol in lipid-rafts. Ionizing radiation, as an extrinsic stressor, causes the separation of β1 integrins from cholesterol lipid raft suggesting that the effects of ionizing radiation on the clustering of β1 integrins are lipid-raft independent.http://link.springer.com/article/10.1186/s13104-017-3032-8Lipid raftMembrane dynamicsIntegrin3D cell cultureSingle molecule microscopy |
| spellingShingle | Laura Babel Larissa Kruse Steven Bump Markus Langhans Tobias Meckel Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions BMC Research Notes Lipid raft Membrane dynamics Integrin 3D cell culture Single molecule microscopy |
| title | Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions |
| title_full | Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions |
| title_fullStr | Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions |
| title_full_unstemmed | Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions |
| title_short | Lipid-rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions |
| title_sort | lipid rafts remain stable even after ionizing radiation induced disintegration of β1 integrin containing focal adhesions |
| topic | Lipid raft Membrane dynamics Integrin 3D cell culture Single molecule microscopy |
| url | http://link.springer.com/article/10.1186/s13104-017-3032-8 |
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