Comparison of Total Oxidative Stress, Total Antioxidant Capacity, and Paraoxonase, Arylesterase, and Lipid Peroxidase Levels in Aqueous Humor and Serum of Diabetic and Non-Diabetic Patients with Catarac

Pur po se: The aim was to compare total oxidative stress (TOS), total antioxidant capacity (TAC), and paraoxonase (PON1), arylesterase (ARE), and lipid peroxidase (LPO) levels in the aqueous humor and concurrent venous blood serum and to evaluate the role of oxidative stress in the progression of...

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Main Authors: Cengiz Caner, Ayşe Vural Özeç, Hüseyin Aydın, Ayşen Topalkara, Mustafa Kemal Arıcı, Haydar Erdoğan, Mustafa İlker Toker
Format: Article
Language:English
Published: Galenos Yayinevi 2012-01-01
Series:Türk Oftalmoloji Dergisi
Subjects:
Online Access:http://www.oftalmoloji.org/article_2124/Comparison-Of-Total-Oxidative-Stress-Total-Antioxidant-Capacity-And-Paraoxonase-Arylesterase-And-Lipid-Peroxidase-Levels-In-Aqueous-Humor-And-Serum-Of-Diabetic-And-Non-diabetic-Patients-With-Cataract
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author Cengiz Caner
Ayşe Vural Özeç
Hüseyin Aydın
Ayşen Topalkara
Mustafa Kemal Arıcı
Haydar Erdoğan
Mustafa İlker Toker
author_facet Cengiz Caner
Ayşe Vural Özeç
Hüseyin Aydın
Ayşen Topalkara
Mustafa Kemal Arıcı
Haydar Erdoğan
Mustafa İlker Toker
author_sort Cengiz Caner
collection DOAJ
description Pur po se: The aim was to compare total oxidative stress (TOS), total antioxidant capacity (TAC), and paraoxonase (PON1), arylesterase (ARE), and lipid peroxidase (LPO) levels in the aqueous humor and concurrent venous blood serum and to evaluate the role of oxidative stress in the progression of diabetic retinopathy in diabetic and non-diabetic patients with cataract. Ma te ri al and Met hod: Oxidative tissue and organ damage play a significant role in diabetes and related complications. A reduction in the activities of antioxidant enzymes such as superoxide dismutase, glutathione peroxidase, glutathione reductase and catalase has been reported in both blood and lens samples. PON1, ARE, and LPO are unreviewed antioxidant enzymes until now. We included diabetic and non-diabetic patients who were admitted to our clinic for complaints of hazy vision and who received the diagnosis of cataract. Patients were divided into 3 groups: group 1 - diabetic patients without retinopathy, group 2- with diabetic retinopathy, and group 3 - patients without systemic disease outside the diagnosis of cataract. A total of 78 patients (26 patients in each group) were enrolled in the study. Re sults: In this study, there was no statistically significant difference among the groups for TOS, TAC, PON1, ARE and LPO levels in aqueous humor. Although simultaneous venous blood serum results, TOS, TAC, PON1 and HbA1c levels in all 3 groups were found statistically significant, ARE and LPO were not statistically significant. When groups 1 and 2 were compared with control group, it was found that in group 1, PON 1 was statistically significantly low and HbA1c was high, while in group 2, TOS and HbA1c were high and TAC and PON 1 were low (p<0.05). Dis cus si on: Oxidative stress indicator enzymes were determined to be positive in blood and negative in humor aquosus. In the present study, these enzymes were studied for the first time in aqueous humor, thus, further studies including subtypes in case of polymorphism are needed. (Turk J Ophthalmol 2012; 42: 47-52)
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spelling doaj.art-b5ef83ec8ba24ada906717434aa6cd832023-02-15T16:07:56ZengGalenos YayineviTürk Oftalmoloji Dergisi1300-06592147-26612012-01-01421475210.4274/tjo.42.74318Comparison of Total Oxidative Stress, Total Antioxidant Capacity, and Paraoxonase, Arylesterase, and Lipid Peroxidase Levels in Aqueous Humor and Serum of Diabetic and Non-Diabetic Patients with CataracCengiz Caner0Ayşe Vural Özeç1Hüseyin Aydın2 Ayşen Topalkara3Mustafa Kemal Arıcı4Haydar Erdoğan5Mustafa İlker Toker6Cumhuriyet Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Sivas, TürkiyeCumhuriyet Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Sivas, TürkiyeCumhuriyet Üniversitesi Tıp Fakültesi, Biyokimya Anabilim Dalı, Sivas, TürkiyeCumhuriyet Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Sivas, TürkiyeCumhuriyet Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Sivas, TürkiyeCumhuriyet Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Sivas, TürkiyeCumhuriyet Üniversitesi Tıp Fakültesi, Göz Hastalıkları Anabilim Dalı, Sivas, TürkiyePur po se: The aim was to compare total oxidative stress (TOS), total antioxidant capacity (TAC), and paraoxonase (PON1), arylesterase (ARE), and lipid peroxidase (LPO) levels in the aqueous humor and concurrent venous blood serum and to evaluate the role of oxidative stress in the progression of diabetic retinopathy in diabetic and non-diabetic patients with cataract. Ma te ri al and Met hod: Oxidative tissue and organ damage play a significant role in diabetes and related complications. A reduction in the activities of antioxidant enzymes such as superoxide dismutase, glutathione peroxidase, glutathione reductase and catalase has been reported in both blood and lens samples. PON1, ARE, and LPO are unreviewed antioxidant enzymes until now. We included diabetic and non-diabetic patients who were admitted to our clinic for complaints of hazy vision and who received the diagnosis of cataract. Patients were divided into 3 groups: group 1 - diabetic patients without retinopathy, group 2- with diabetic retinopathy, and group 3 - patients without systemic disease outside the diagnosis of cataract. A total of 78 patients (26 patients in each group) were enrolled in the study. Re sults: In this study, there was no statistically significant difference among the groups for TOS, TAC, PON1, ARE and LPO levels in aqueous humor. Although simultaneous venous blood serum results, TOS, TAC, PON1 and HbA1c levels in all 3 groups were found statistically significant, ARE and LPO were not statistically significant. When groups 1 and 2 were compared with control group, it was found that in group 1, PON 1 was statistically significantly low and HbA1c was high, while in group 2, TOS and HbA1c were high and TAC and PON 1 were low (p<0.05). Dis cus si on: Oxidative stress indicator enzymes were determined to be positive in blood and negative in humor aquosus. In the present study, these enzymes were studied for the first time in aqueous humor, thus, further studies including subtypes in case of polymorphism are needed. (Turk J Ophthalmol 2012; 42: 47-52)http://www.oftalmoloji.org/article_2124/Comparison-Of-Total-Oxidative-Stress-Total-Antioxidant-Capacity-And-Paraoxonase-Arylesterase-And-Lipid-Peroxidase-Levels-In-Aqueous-Humor-And-Serum-Of-Diabetic-And-Non-diabetic-Patients-With-CataractDiabetic retinopathyaqueous humoroxidative stressparaoxonasearylesterase
spellingShingle Cengiz Caner
Ayşe Vural Özeç
Hüseyin Aydın
Ayşen Topalkara
Mustafa Kemal Arıcı
Haydar Erdoğan
Mustafa İlker Toker
Comparison of Total Oxidative Stress, Total Antioxidant Capacity, and Paraoxonase, Arylesterase, and Lipid Peroxidase Levels in Aqueous Humor and Serum of Diabetic and Non-Diabetic Patients with Catarac
Türk Oftalmoloji Dergisi
Diabetic retinopathy
aqueous humor
oxidative stress
paraoxonase
arylesterase
title Comparison of Total Oxidative Stress, Total Antioxidant Capacity, and Paraoxonase, Arylesterase, and Lipid Peroxidase Levels in Aqueous Humor and Serum of Diabetic and Non-Diabetic Patients with Catarac
title_full Comparison of Total Oxidative Stress, Total Antioxidant Capacity, and Paraoxonase, Arylesterase, and Lipid Peroxidase Levels in Aqueous Humor and Serum of Diabetic and Non-Diabetic Patients with Catarac
title_fullStr Comparison of Total Oxidative Stress, Total Antioxidant Capacity, and Paraoxonase, Arylesterase, and Lipid Peroxidase Levels in Aqueous Humor and Serum of Diabetic and Non-Diabetic Patients with Catarac
title_full_unstemmed Comparison of Total Oxidative Stress, Total Antioxidant Capacity, and Paraoxonase, Arylesterase, and Lipid Peroxidase Levels in Aqueous Humor and Serum of Diabetic and Non-Diabetic Patients with Catarac
title_short Comparison of Total Oxidative Stress, Total Antioxidant Capacity, and Paraoxonase, Arylesterase, and Lipid Peroxidase Levels in Aqueous Humor and Serum of Diabetic and Non-Diabetic Patients with Catarac
title_sort comparison of total oxidative stress total antioxidant capacity and paraoxonase arylesterase and lipid peroxidase levels in aqueous humor and serum of diabetic and non diabetic patients with catarac
topic Diabetic retinopathy
aqueous humor
oxidative stress
paraoxonase
arylesterase
url http://www.oftalmoloji.org/article_2124/Comparison-Of-Total-Oxidative-Stress-Total-Antioxidant-Capacity-And-Paraoxonase-Arylesterase-And-Lipid-Peroxidase-Levels-In-Aqueous-Humor-And-Serum-Of-Diabetic-And-Non-diabetic-Patients-With-Cataract
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